浙江农业科学

浙江农业科学 ›› 2026, Vol. 67 ›› Issue (7): 1675-1679.DOI: 10.16178/j.issn.0528-9017.20250641

• 薯类作物 • 上一篇    下一篇

心香甘薯茎尖脱毒组织培养的高效快繁体系建立

全汝青1(), 陈科2, 荣松1, 叶雄方1, 米晓洁1, 杨哲1()   

  1. 1.浙江传化生物技术有限公司 浙江 杭州 311231
    2.浙江传化乡村发展有限公司 浙江 杭州 311200
  • 收稿日期:2025-09-04 出版日期:2026-07-11 发布日期:2026-07-27
  • 通讯作者: 杨哲
  • 作者简介:杨哲，E-mail：yz_ccl@sina.com。
    全汝青，研究方向为农业育种。E-mail：1853846283@qq.com。

Establishment of an efficient and rapid propagation system for virus-free tissue culture of Xinxiang sweet potato stem tips

QUAN Ruqing1(), CHEN Ke2, RONG Song1, YE Xiongfang1, MI Xiaojie1, YANG Zhe1()   

  1. 1.Zhejiang Transfar Biotechnology Co. ，Ltd. ，Hangzhou 311231，Zhejiang
    2.Zhejiang Transfar Rural Development Co. ，Ltd. ，Hangzhou 311200，Zhejiang
  • Received:2025-09-04 Online:2026-07-11 Published:2026-07-27
  • Contact: YANG Zhe

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摘要：

为建立甘薯茎尖脱毒组织培养的高效快繁体系，本研究以心香甘薯为材料，通过设计6种外植体消毒处理、10种含不同质量浓度6-苄氨基嘌呤（6-BA）、萘乙酸（NAA）、赤霉素（GA3）的MS培养基，以及3种蔗糖质量浓度梯度试验，系统探究不同因素对茎尖脱毒组织的影响。结果表明，外植体消毒以75.0%乙醇+0.2% NaClO处理6 min为最佳，该处理下的茎尖组织污染率为17.8%，成活率为62.2%，兼顾杀菌效果与外植体活性。正交试验结果表明，A1培养基（MS+0.50 mg·L-1 6-BA+0.10 mg·L-1 NAA）的愈伤诱导率最高（80.00%），B1培养基（MS+0.05 mg·L-1 6-BA+0.10 mg·L-1 GA3）的生根率最高（73.33%），C3培养基（MS+1.00 mg·L-1 6-BA+0.20 mg·L-1 NAA+0.20 mg·L-1 GA3）的萌芽率（62.22%）和成苗率（55.56%）最高。蔗糖质量浓度以25 g·L-1最优，该处理下的萌芽率为60.00%，成苗率为55.56%，植株生长健壮。此外，组培苗扩繁以1/2 MS+0.10 g·L-1花宝5号培养基的效果最佳，经检测，脱毒苗心香甘薯的甘薯羽状斑驳病毒（SPFMV）和甘薯褪绿矮化病毒（SPCSV）都是阴性，脱毒率达到100%。本研究筛选的消毒方案、培养基配方及蔗糖质量浓度，为心香脱毒苗的产业化生产提供了技术支撑。

关键词: 甘薯, 茎尖, 脱毒, 组织培养, 高效快繁体系

Abstract:

To establish an efficient and rapid propagation system for virus-free stem tip tissue of sweet potato，this study utilized sweet potato variety Xinxiang as the material and conducted a systematic investigation into the effects of various factors on virus-free tissue of Xinxiang sweet potato stem tips. The experiments included six disinfection protocols for explants，ten MS culture media containing different mass concentrations of 6-BA，NAA，and GA3，as well as three sucrose mass concentration gradient treatments. Results showed that the optimal explant disinfection was 6 minutes of 75.0% ethanol+0.2% sodium hypochlorite，achieving a contamination rate of 17.8% and survival rate of 62.2%，balancing sterilization effectiveness with explant viability. Hormone tests revealed that A1 medium（MS+0.50 mg·L-1 6-BA+0.10 mg·L-1 NAA）had the highest callus induction rate（80.00%），B1 medium（MS+0.05 mg·L-1 6-BA+0.10 mg·L-1 GA3）showed the highest rooting rate（73.33%），C3 medium（MS+1.00 mg·L-1 6-BA+0.20 mg·L-1 NAA+0.20 mg·L-1 GA3）demonstrated the best germination rate（62.22%），and achieved the highest seedling survival rate（55.56%）. The optimal sucrose mass concentration was 25 g·L-1，yielding a 60.00% bud germination rate and 55.56% seedling survival rate，with robust plant growth. Furthermore，the tissue culture propagation of seedlings achieved optimal results using a 1/2 MS medium combined with 0.10 g·L-1 Huabao No.5 culture medium. The virus-free Xinxiang seedlings tested negative for both sweet potato mosaic virus（SPFMV）and sweet potato chlorosis dwarfing virus（SPCSV），with a 100% virus elimination rate. The sterilization protocols，medium formulations，and sucrose mass concentrations identified in this study provide technical support for industrial-scale production of Xinxiang virus-free seedlings.

Key words: sweet potato, stem tip, virus-free, tissue culture, efficient and rapid propagation system

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