草酸对镉污染土壤亚麻根际细菌多样性的影响

doi:10.16178/j.issn.0528-9017.20240275

摘要/Abstract

摘要：

为研究草酸对镉污染土壤中亚麻根际细菌微生物的影响,采用盆栽试验,待亚麻生理成熟期取根际土壤,通过Illumina-MiSeq高通量测序技术,分析不同浓度草酸对镉污染土壤中亚麻根际细菌多样性及群落组成的影响。结果显示,高浓度的草酸处理有助于增加镉污染土壤中亚麻根际细菌的OTU数量2.5 mmol·kg^-1草酸处理下,ACE、Chao1、Shannon和Simpson指数值均为最高,其中,Shannon和Simpson指数值显著大于对照;PCoA分析结果表明,2.5 mmol·kg^-1草酸处理后的亚麻根际土壤细菌OTU组成与对照的土壤细菌OTU组成之间的群落结构差异最大;在细菌组成方面,所有处理的优势菌门和优势菌属分别为髌骨细菌门和TM7a,平均占比分别为40.60%和26.06%。综合来看,2.5 mmol·kg^-1草酸处理能够增加镉污染土壤中亚麻根际细菌的多样性和丰富度,对细菌组成的影响也最大。本研究有助于为后续研究草酸等外源有机酸对亚麻镉吸收的影响提供一定的理论参考。

关键词: 草酸, 亚麻, 根际, 细菌多样性

Abstract:

In order to study the effects of oxalic acid on rhizosphere bacteria of flax in cadmium-contaminated soil, a pot experiment was conducted. The rhizosphere soil of flax was taken at the physiological maturity stage, and the effects of different concentrations of oxalic acid on rhizosphere bacterial diversity and community composition of flax in cadmium-contaminated soil were analyzed by Illumina-MiSeq high-throughput sequencing technology. The results showed that the treatment with high concentration of oxalic acid could increase the OTU number of rhizosphere bacteria in the cadmium-contaminated soil. ACE, Chao1, Simpson and Shannon index values were the highest under the treatment with 2.5 mmol·kg^-1 oxalic acid, among which Simpson and Shannon index values were significantly higher than the control. The results of PCoA analysis showed that the community structure of bacterial OTU in rhizosphere soil of flax treated with high concentration of oxalic acid was the most different from that in control. In terms of bacterial composition, the dominant bacteria phylum and dominant bacteria genus were Patescibacteria and TM7a, respectively, with an average proportion of 40.60% and 26.06%, respectively. In summary, the treatment with 2.5 mmol·kg^-1of oxalic acid can increase the diversity and abundance of rhizosphere bacteria in cadmium-contaminated soil, and has the greatest effect on bacterial composition. This study can be helpful to provide a theoretical reference for the subsequent research on the effects of oxalic acid and other exogenous organic acids on cadmium absorption in flax.

Key words: oxalic acid, flax, rhizosphere, bacterial diversity

中图分类号:

S532

柳婷婷, 邹丽娜, 骆霞虹, 李文略, 陈常理, 朱关林, 安霞. 草酸对镉污染土壤亚麻根际细菌多样性的影响[J]. 浙江农业科学, 2025, 66(7): 1764-1769.

LIU Tingting, ZOU Lina, LUO Xiahong, LI Wenlue, CHEN Changli, ZHU Guanlin, AN Xia. Effects of oxalic acid on rhizosphere bacterial diversity of flax in cadmium-contaminated soil[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(7): 1764-1769.

图/表 6

表1 亚麻根际土壤细菌测序数据统计分析

Table 1 Sequencing data of bacteria in rhizosphere soil of flax

样品名称	有效序列数目/条	总长度/ bp	最大长度/ bp	最小长度/ bp	OTU数量/ 个
CK-1	111 812	51 027 445	474	201	1 872
CK-2	103 777	47 039 247	474	202	2 051
CK-3	101 592	46 218 601	474	202	1 833
OA1-1	109 563	49 807 858	474	201	2 112
OA1-2	111 101	50 507 042	474	202	1 669
OA1-3	98 785	44 790 113	474	201	1 681
OA2-1	99 809	45 142 020	474	202	1 984
OA2-2	102 732	46 493 559	474	202	2 036
OA2-3	109 951	49 747 733	474	202	2 308
OA3-1	103 206	46 739 607	474	202	2 358
OA3-2	103 438	46 926 580	474	206	2 265
OA3-3	99 622	45 013 130	474	202	2 013

图1 不同处理下亚麻根际土壤细菌共有OTU韦恩图

Fig.1 Venn map of common OTU of bacteria in rhizosphere soil of flax under different treatments

表2 亚麻根际土壤细菌α多样性指数分析

Table 2 Analysis of α diversity index of bacteria in rhizosphere soil of flax

样品名称	覆盖度/%	ACE指数	Chao1指数	Shannon指数	Simpson指数
CK	99.29	2 173.90±90.23 ab	2 055.48±100.32 ab	5.99±0.26 b	0.92±0.01 b
OA1	99.28	2 101.83±213.01 b	1 980.94±225.35 b	6.18±0.89 b	0.94±0.03 ab
OA2	99.20	2 383.41±155.70 ab	2 254.19±150.29 ab	6.69±0.37 ab	0.94±0.02 ab
OA3	99.28	2 465.14±183.28 a	2 334.68±165.32 a	7.32±0.49 a	0.96±0.02 a

图2 不同处理下亚麻根际土壤细菌PCoA分析

Fig.2 Principal coordinate analysis of bacteria in rhizosphere soil of flax under different treatments

图3 亚麻根际土壤细菌群落在门水平上的组成和相对丰度

Fig.3 Composition and relative abundance of bacterial communities in rhizosphere soil of flax at phylum level

图4 亚麻根际土壤细菌群落在属水平上的组成和相对丰度

Fig.4 Composition and relative abundance of bacterial communities in rhizosphere soil of flax at genus level

参考文献 21

[1]	周松, 杨健豪, 晏士玮, 等. 根际有机酸对土壤中重金属化学行为和生物有效性的影响研究进展[J]. 生物学杂志, 2022, 39(3): 103-106,124.
[2]	HALDAR S, SENGUPTA S. Plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential[J]. The Open Microbiology Journal, 2015, 9: 1-7.
[3]	吴林坤, 林向民, 林文雄. 根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J]. 植物生态学报, 2014, 38(3): 298-310.
[4]	丁永祯, 李志安, 邹碧. 土壤低分子量有机酸及其生态功能[J]. 土壤, 2005, 37(3): 243-250.
[5]	赵宽, 万昕, 邢德科, 等. 低分子量有机酸对土壤有效磷及重金属释放影响的研究进展[J]. 土壤通报, 2022, 53(5): 1228-1236.
[6]	詹淑威, 潘伟斌, 赖彩秀, 等. 外源有机酸对小飞扬草(Euphorbia thymifolia L.)修复镉污染土壤的影响[J]. 环境工程学报, 2015, 9(10): 5096-5102.
[7]	薛博晗, 李娜, 宋桂龙, 等. 外源柠檬酸、苹果酸和草酸对披碱草镉耐受及富集的影响[J]. 草业学报, 2018, 27(6): 128-136.
[8]	王玉富, 郭媛, 汤清明, 等. 亚麻修复重金属污染土壤的研究与应用[J]. 作物研究, 2015, 29(4): 443-448.
[9]	柳婷婷, 骆霞虹, 李文略, 等. 不同亚麻品种重金属Cd吸收积累的差异[J]. 浙江农业科学, 2022, 63(9): 2030-2032, 2045.
[10]	EDWARDS J, JOHNSON C, SANTOS- MEDELLÍN C, et al. Structure, variation, and assembly of the root-associated microbiomes of rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(8): E911-E920.
[11]	CHEN S F, ZHOU Y Q, CHEN Y R, et al. Fastp: an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 2018, 34(17): i884-i890.
[12]	MAGOČ T, SALZBERG S L. FLASH: fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics, 2011, 27(21): 2957-2963.
[13]	EDGAR R C. UPARSE: highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods, 2013, 10(10): 996-998.
[14]	EDGAR R C, HAAS B J, CLEMENTE J C, et al. UCHIME improves sensitivity and speed of Chimera detection[J]. Bioinformatics, 2011, 27(16): 2194-2200.
[15]	张东艳, 赵建, 杨水平, 等. 川明参轮作对烟地土壤微生物群落结构的影响[J]. 中国中药杂志, 2016, 41(24): 4556-4563.
[16]	艾超, 孙静文, 王秀斌, 等. 植物根际沉积与土壤微生物关系研究进展[J]. 植物营养与肥料学报, 2015, 21(5): 1343-1351.
[17]	刘良茜, 陶怡, 方林平, 等. 野生金线莲根际土壤微生物分析[J]. 分子植物育种, 2023, 21(24): 8210-8217.
[18]	赵娅红, 吴治兴, 刘敏荣, 等. 菊花枯萎病植株根际土壤细菌群落多样性比较[J]. 中国农学通报, 2023, 39(13): 109-116.
[19]	GIRKIN N T, TURNER B L, OSTLE N, et al. Composition and concentration of root exudate analogues regulate greenhouse gas fluxes from tropical peat[J]. Soil Biology and Biochemistry, 2018, 127: 280-285.
[20]	王水良, 王平, 王趁义. 铝胁迫下马尾松幼苗有机酸分泌和根际pH值的变化[J]. 生态与农村环境学报, 2010, 26(1): 87-91.
[21]	张晓波, 于春雷, 张文洋, 等. 不同连作年限菊花根际土壤微生物多样性变化特征[J]. 浙江农业学报, 2022, 34(12): 2710-2719.