浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (8): 1840-1845.DOI: 10.16178/j.issn.0528-9017.20240764

• 水产科学 • 上一篇    下一篇

罗氏沼虾育苗期优势细菌调查及耐药性分析

郭琦1(), 吕孙建1, 沈卫锋1, 朱东敏2, 刘莉1,*()   

  1. 1.浙江省农业科学院 水生生物研究所,浙江 杭州 310021
    2.宁波甬港水产种苗科技有限公司,浙江 宁波 315703
  • 收稿日期:2024-09-24 出版日期:2025-08-11 发布日期:2025-09-04
  • 通讯作者: 刘莉,E-mail: liuli6655@hotmail.com。
  • 作者简介:郭琦(1991—),女,浙江杭州人,助理研究员,博士,主要从事水产病害研究,E-mail: guoqi@zju.edu.cn。
  • 基金资助:
    2024年度宁波市“科创甬江2035”关键技术突破计划项目(2024Z278);浙江省科技计划重点项目(2022C02027)

Investigation and antibiotic sensitivity analysis of dominant bacteria isolated from breeding stage of Macrobrachium rosenbergii

GUO Qi1(), LYU Sunjian1, SHEN Weifeng1, ZHU Dongmin2, LIU Li1,*()   

  1. 1. Institute of Hydrobiology, Zhejiang Academic of Agricultural Sciences, Hangzhou 310021, Zhejiang
    2. Ningbo Yonggang Aquatic Seedling Technology Co., Ltd., Ningbo 315703, Zhejiang
  • Received:2024-09-24 Online:2025-08-11 Published:2025-09-04
  • Contact: 刘莉,E-mail: liuli6655@hotmail.com。

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摘要：

育苗期的条件致病菌感染是影响罗氏沼虾(Macrobrachium rosenbergii)出苗率的重要原因之一。本研究从4个育苗场中定期采集幼体、虾苗及其养殖水,采用琼脂平板分离培养后对样品的细菌数量进行调查,对分离到的优势细菌进行16S rRNA序列分析,并对优势菌群进行耐药性分析。结果显示,育苗期间虾苗及幼体中的细菌数量处于较高水平,优势细菌的变化与季节存在一定联系,其中产气克雷伯氏菌、弧菌属和气单胞菌属细菌较为普遍。优势菌群的耐药性分析结果显示,不同育苗场虾苗中的优势菌对头孢氨苄和利福平表现出耐药性,对其他测试抗生素的敏感性存在差异。本研究结果为罗氏沼虾育苗期细菌感染的针对性防控提供了参考依据。

关键词: 罗氏沼虾, 育苗期, 优势菌, 药敏分析, 16S rRNA

Abstract:

As the bacteria infection in breeding stage of Macrobrachium rosenbergii could influence the metamorphosis rate of zoea, we collected the zoea, postlarva and their rearing water from four hatcheries at different time points during the breeding stage. The agar plate was used to isolate and culture to investigate the amounts of bacteria in the samples, the 16S rRNA sequence of the isolated dominant bacteria was analyzed, and the drug resistance of the dominant bacteria was analyzed. The results showed that the amounts of bacteria in postlarva and zoea were at a high level, and the variation of dominant bacteria was related to the season. Klebsiella aerogenes, Vibrio sp. and Aeromonas sp. were relatively common in the collected samples from these four hatcheries. The antibiotic sensitivity detection showed that the dominant bacteria of postlarva were all resistant to cephalexin and rifampicin, while the sensitive antibiotics were varied from different hatcheries. The results of this study provided a reference for the targeted prevention and control of bacterial infection in the breeding stage of Macrobrachium rosenbergii.

Key words: Macrobrachium rosenbergii, breeding stage, dominant bacteria, antibiotic sensitivity detection, 16S rRNA

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