Genetic diversity analysis and control strategies of weedy rice in Huzhou Area

doi:10.16178/j.issn.0528-9017.20220963

Abstract

Abstract:

Weedy rice, as a genetically close weed of rice, is one of the most difficult weeds to control in rice fields. With the advancement of modern agriculture, the area of rice direct seeding has increased year by year, and the harm of weed rice to rice yield has become increasingly serious. In this study, 48 pairs of SSR primers specified in NY/T 1433—2014 were used to analyze the genetic diversity of 15 weed rice plants collected from Huzhou City, and their phenotypic traits were analyzed and compared. The results showed that the phenotypic differences of the 15 weed rice plants were significant, and the grain drop was significantly stronger than that of the control Jietian rice 001 and Wuyunjing. The SSR results showed that a total of 104 alleles were detected by 48 pairs of primers, and the average polymorphic information was 0.313 8, showing moderate polymorphism. The average number of alleles was 2.312 5, the average number of effective alleles was 1.577 2, and the average Shannon information index was 0.515 4, indicating high genetic diversity. In this study, ALS gene sequence analysis was performed on these 15 weedy rice lines, and 32 SNP loci were found, but none of them produced resistance. Therefore, the control strategy of weedy rice in Huzhou was preliminarily discussed by using rice resistant to ALS inhibitor herbicides. The results showed that the weeds found in Huzhou could be controlled by spraying ALS inhibitor herbicides. These results will provide a reference for the control strategy of weed rice in Huzhou.

Key words: simple repeats, weedy rice, genetic diversity, Huzhou, acetyllactate synthase

CLC Number:

S545

ZHAO Deqi, GU Gang, MENG Huabing, ZHANG Qiyan, SHANG Nianjun, CHEN Wei, CHEN Lei. Genetic diversity analysis and control strategies of weedy rice in Huzhou Area[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(1): 148-156.

Figures/Tables 9

References 16

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编号	株叶形	株高/cm	播始历期/d	芒	谷粒形状	种皮颜色	粒长/mm	粒宽/mm	长宽比	穗-粒分离力/N
W1	较松散	94.88	87	短芒	椭圆形	红色	7.6	3.0	2.5	0.78
W2	较松散	93.50	89	短芒	椭圆形	红色	7.4	3.0	2.5	0.70
W3	较松散	93.94	86	短芒	椭圆形	红色	7.0	2.9	2.4	0.69
W4	较松散	95.90	88	短芒	椭圆形	红色	7.4	2.9	2.6	0.79
W5	松散	96.80	90	短芒	椭圆形	红色	7.5	2.8	2.7	0.75
W6	松散	103.86	75	短芒	椭圆形	红色	8.1	3.1	2.6	0.37
W7	紧凑	89.92	72	无	阔卵形	红色	6.2	3.0	2.1	0.72
W8	较松散	100.56	89	无	椭圆形	红色	7.8	3.0	2.6	0.40
W9	较松散	110.02	82	无	椭圆形	红色	8.1	3.2	2.5	0.28
W10	较松散	106.74	87	长芒	椭圆形	红色	7.5	3.2	2.3	0.77
W11	较松散	93.22	92	短芒	椭圆形	红色	7.6	3.0	2.5	0.62
W12	松散	111.46	77	无	椭圆形	红色	7.9	2.8	2.8	0.50
W13	较松散	102.20	91	长芒	椭圆形	红色	7.9	2.7	2.9	0.74
W14	松散	92.20	86	无	椭圆形	红色	7.6	3.1	2.5	0.59
W15	较松散	104.52	87	长芒	椭圆形	红色	7.4	2.8	2.6	0.67
洁田稻001	较松散	110.07	95	无	细长型	白色	9.3	2.1	4.4	1.18^*
武运粳	紧凑	95.35	118	无	椭圆形	白色	7.6	3.2	2.4	1.68^*

编号	株叶形	株高/cm	播始历期/d	芒	谷粒形状	种皮颜色	粒长/mm	粒宽/mm	长宽比	穗-粒分离力/N
W1	较松散	94.88	87	短芒	椭圆形	红色	7.6	3.0	2.5	0.78
W2	较松散	93.50	89	短芒	椭圆形	红色	7.4	3.0	2.5	0.70
W3	较松散	93.94	86	短芒	椭圆形	红色	7.0	2.9	2.4	0.69
W4	较松散	95.90	88	短芒	椭圆形	红色	7.4	2.9	2.6	0.79
W5	松散	96.80	90	短芒	椭圆形	红色	7.5	2.8	2.7	0.75
W6	松散	103.86	75	短芒	椭圆形	红色	8.1	3.1	2.6	0.37
W7	紧凑	89.92	72	无	阔卵形	红色	6.2	3.0	2.1	0.72
W8	较松散	100.56	89	无	椭圆形	红色	7.8	3.0	2.6	0.40
W9	较松散	110.02	82	无	椭圆形	红色	8.1	3.2	2.5	0.28
W10	较松散	106.74	87	长芒	椭圆形	红色	7.5	3.2	2.3	0.77
W11	较松散	93.22	92	短芒	椭圆形	红色	7.6	3.0	2.5	0.62
W12	松散	111.46	77	无	椭圆形	红色	7.9	2.8	2.8	0.50
W13	较松散	102.20	91	长芒	椭圆形	红色	7.9	2.7	2.9	0.74
W14	松散	92.20	86	无	椭圆形	红色	7.6	3.1	2.5	0.59
W15	较松散	104.52	87	长芒	椭圆形	红色	7.4	2.8	2.6	0.67
洁田稻001	较松散	110.07	95	无	细长型	白色	9.3	2.1	4.4	1.18^*
武运粳	紧凑	95.35	118	无	椭圆形	白色	7.6	3.2	2.4	1.68^*

引物	PIC	N_a	N_e	I	引物	PIC	N_a	N_e	I
RM583	0.456 1	3	2.227 7	0.882 3	RM493	0.440 3	3	2.102 8	0.853 2
RM71	0.226 6	3	1.315 8	0.485 1	RM561	0.373 9	2	1.991 2	0.690 9
RM85	0.495 6	3	2.102 8	0.853 2	RM8277	0.204 4	2	1.300 6	0.392 7
RM471	0.345 7	2	1.800 0	0.636 5	RM551	0.364 8	2	1.923 1	0.673 0
RM274	0.116 7	2	1.142 1	0.244 9	RM598	0.204 4	2	1.300 6	0.392 7
RM190	0.204 4	2	1.300 6	0.392 7	RM176	单态位点	1	1.000 0	0
RM336	0.440 3	3	2.102 8	0.853 2	RM432	0.440 3	3	2.102 8	0.853 2
RM72	0.116 7	2	1.142 1	0.244 9	RM331	0.116 7	2	1.142 1	0.244 9
RM219	0.509 4	3	2.419 4	0.970 1	OSR28	0.412 2	3	1.923 1	0.803 3
RM311	0.204 4	2	1.300 6	0.392 7	RM590	0.373 9	2	1.991 2	0.690 9
RM209	0.226 6	3	1.315 8	0.485 1	RM21	0.309 2	3	1.510 1	0.627 7
RM19	0.110 3	2	1.068 9	0.146 1	RM3331	0.314 6	2	1.642 3	0.579 9
RM1195	0.445 0	3	1.991 2	0.860 9	RM443	单态位点	1	1.000 0	0
RM208	0.329 5	4	1.530 6	0.720 1	RM490	0.214 6	3	1.226 2	0.388 7
RM232	0.484 6	3	2.227 7	0.927 6	RM424	0.116 7	2	1.142 1	0.244 9
RM119	0.364 8	2	1.923 1	0.673 0	RM423	0.314 6	2	1.642 3	0.579 9
RM267	单态位点	1	1.000 0	0	RM571	0.268 8	2	1.470 6	0.500 4
RM253	0.412 2	3	1.923 1	0.803 3	RM231	0.345 7	2	1.800 0	0.636 5
RM481	0.581 1	5	2.710 8	1.229 3	RM567	0.268 8	2	1.470 6	0.500 4
RM339	单态位点	1	1.000 0	0	RM289	0.116 7	2	1.142 1	0.244 9
RM278	0.412 2	3	1.923 1	0.803 3	RM542	0.116 7	2	1.142 1	0.244 9
RM258	0.204 4	2	1.300 6	0.392 7	RM316	单态位点	1	1.000 0	0
RM224	0.440 3	3	2.102 8	0.853 2	RM332	0.420 0	3	1.867 2	0.744 9
RM17	单态位点	1	1.000 0	0	RM7102	单态位点	1	1.000 0	0
					平均	0.313 8	2.312 5	1.577 2	0.515 4

引物	PIC	N_a	N_e	I	引物	PIC	N_a	N_e	I
RM583	0.456 1	3	2.227 7	0.882 3	RM493	0.440 3	3	2.102 8	0.853 2
RM71	0.226 6	3	1.315 8	0.485 1	RM561	0.373 9	2	1.991 2	0.690 9
RM85	0.495 6	3	2.102 8	0.853 2	RM8277	0.204 4	2	1.300 6	0.392 7
RM471	0.345 7	2	1.800 0	0.636 5	RM551	0.364 8	2	1.923 1	0.673 0
RM274	0.116 7	2	1.142 1	0.244 9	RM598	0.204 4	2	1.300 6	0.392 7
RM190	0.204 4	2	1.300 6	0.392 7	RM176	单态位点	1	1.000 0	0
RM336	0.440 3	3	2.102 8	0.853 2	RM432	0.440 3	3	2.102 8	0.853 2
RM72	0.116 7	2	1.142 1	0.244 9	RM331	0.116 7	2	1.142 1	0.244 9
RM219	0.509 4	3	2.419 4	0.970 1	OSR28	0.412 2	3	1.923 1	0.803 3
RM311	0.204 4	2	1.300 6	0.392 7	RM590	0.373 9	2	1.991 2	0.690 9
RM209	0.226 6	3	1.315 8	0.485 1	RM21	0.309 2	3	1.510 1	0.627 7
RM19	0.110 3	2	1.068 9	0.146 1	RM3331	0.314 6	2	1.642 3	0.579 9
RM1195	0.445 0	3	1.991 2	0.860 9	RM443	单态位点	1	1.000 0	0
RM208	0.329 5	4	1.530 6	0.720 1	RM490	0.214 6	3	1.226 2	0.388 7
RM232	0.484 6	3	2.227 7	0.927 6	RM424	0.116 7	2	1.142 1	0.244 9
RM119	0.364 8	2	1.923 1	0.673 0	RM423	0.314 6	2	1.642 3	0.579 9
RM267	单态位点	1	1.000 0	0	RM571	0.268 8	2	1.470 6	0.500 4
RM253	0.412 2	3	1.923 1	0.803 3	RM231	0.345 7	2	1.800 0	0.636 5
RM481	0.581 1	5	2.710 8	1.229 3	RM567	0.268 8	2	1.470 6	0.500 4
RM339	单态位点	1	1.000 0	0	RM289	0.116 7	2	1.142 1	0.244 9
RM278	0.412 2	3	1.923 1	0.803 3	RM542	0.116 7	2	1.142 1	0.244 9
RM258	0.204 4	2	1.300 6	0.392 7	RM316	单态位点	1	1.000 0	0
RM224	0.440 3	3	2.102 8	0.853 2	RM332	0.420 0	3	1.867 2	0.744 9
RM17	单态位点	1	1.000 0	0	RM7102	单态位点	1	1.000 0	0
					平均	0.313 8	2.312 5	1.577 2	0.515 4

	W1	W2	W3	W4	W5	W6	W7	W8	W9	W10	W11	W12	W13	W14
W2	1.000 0
W3	1.000 0	1.000 0
W4	1.000 0	1.000 0	1.000 0
W5	0.979 6	0.979 6	0.979 6	0.979 6
W6	0.453 6	0.453 6	0.453 6	0.453 6	0.474 2
W7	0.525 3	0.525 3	0.525 3	0.525 3	0.505 1	0.449 0
W8	0.632 7	0.632 7	0.632 7	0.632 7	0.612 2	0.536 1	0.444 4
W9	0.577 3	0.577 3	0.577 3	0.577 3	0.556 7	0.562 5	0.449 0	0.659 8
W10	0.536 1	0.536 1	0.536 1	0.536 1	0.515 5	0.500 0	0.387 8	0.577 3	0.729 2
W11	0.959 2	0.959 2	0.959 2	0.959 2	0.979 6	0.453 6	0.484 8	0.591 8	0.536 1	0.494 8
W12	0.577 3	0.577 3	0.577 3	0.577 3	0.556 7	0.562 5	0.469 4	0.680 4	0.979 2	0.750 0	0.536 1
W13	0.959 2	0.959 2	0.959 2	0.959 2	0.979 6	0.453 6	0.484 8	0.591 8	0.536 1	0.494 8	1.000 0	0.536 1
W14	0.577 3	0.577 3	0.577 3	0.577 3	0.556 7	0.562 5	0.469 4	0.680 4	0.979 2	0.750 0	0.536 1	1.000 0	0.536 1
W15	0.959 2	0.959 2	0.959 2	0.959 2	0.979 6	0.453 6	0.484 8	0.591 8	0.536 1	0.494 8	1.000 0	0.536 1	1.000 0	0.536 1