Tolerance and culture condition optimization of hypoglycemic Lactobacillus rhamnosus

doi:10.16178/j.issn.0528-9017.20240204

Abstract

Abstract:

This study compared the hypoglycemic abilities of three strains of Lactobacillus rhamnosus and selected Lactobacillus rhamnosus JYLR-005，which demonstrated the best hypoglycemic function，for further investigation. The tolerance characteristics of Lactobacillus rhamnosus JYLR-005 were evaluated through acid tolerance，bile salt tolerance，and survival rates in simulated intestinal fluid and simulated gastric fluid. Additionally，the culture conditions for the strain were optimized. The results indicated that Lactobacillus rhamnosus JYLR-005 could grow in media with pH values ranging from 1.5 to 6.5，showing a higher survival rate at pH value 6.5，which increased over time. The strain could survive under ox bile salt concentrations of 3-9 mg·L?1，indicating a certain level of bile salt tolerance. The survival rates of Lactobacillus rhamnosus JYLR-005 in simulated gastric fluid（pH value 1.5）and simulated intestinal fluid（pH value 6.8）gradually decreased over time. Orthogonal experiments revealed that the optimal culture conditions for Lactobacillus rhamnosus JYLR-005 were pH value 7.5，temperature 40 °C，inoculum size 5%，and incubation time 54 h.

Key words: Lactobacillus rhamnosus, hypoglycemia, tolerance, survival rate

CLC Number:

Q939.11

WU Shuting, WU Pengfei, CHEN Chunying, ZHANG Yuqing, XING Jinzhan, AN Lixia, SUN Yali. Tolerance and culture condition optimization of hypoglycemic Lactobacillus rhamnosus[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(3): 726-731.

Figures/Tables 11

Table 1 Single factor experiment design

温度/℃	接种量/%	pH值	时间/h
30	2	4	15
35	3	5	30
40	4	6	45
45	5	7	60
50	6	8	75

Table 2 Orthogonal experimental design

水平	温度/℃	接种量/%	pH值	时间/h
1	35	3	6.5	54
2	40	4	7.0	60
3	45	5	7.5	66

Fig.1 Colony morphology of Lactobacillusrhamnosus JYLR

Fig.2 Gram staining morphology of Lactobacillus rhamnosus JYLR

Fig.3 Inhibition rate of α-amylase by Lactobacillus rhamnosus JYLR

Fig.4 Survival rate of Lactobacillus rhamnosus JYLR-005 under different pH values

Fig.5 The viable count of Lactobacillus rhamnosus JYLR-005 at different concentrations of bovine bile salts

Fig.6 Survival rate of Lactobacillus rhamnosus JYLR-005 in simulated intestinal fluid and simulated gastric fluid

Fig. 7 Growth of Lactobacillus rhamnosus JYLR-005 under different conditions

Table 3 Orthogonal test results

序号	A	B	C	D	D₅₄₀
1	1	1	1	1	2.673
2	1	2	2	2	2.602
3	1	3	3	3	2.741
4	2	1	2	3	2.658
5	2	2	3	1	2.825
6	2	3	1	2	2.783
7	3	1	3	2	2.722
8	3	2	1	3	2.628
9	3	3	2	1	2.677

Table 4 Range analysis results

因素	k₁	k₂	k₃	R	最优水平
A	2.672	2.756	2.676	0.084	2
B	2.685	2.685	2.734	0.049	3
C	2.695	2.646	2.763	0.117	3
D	2.725	2.702	2.676	0.049	1

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