Analysis of avirulence genes in Magnaporthe oryzae in Zhejiang Province

doi:10.16178/j.issn.0528-9017.20240422

Abstract

Abstract:

PCR detection was performed on 170 Magnaporthe oryza strains collected from various rice-growing regions in Zhejiang Province using specific primers for seven avirulence genes. The results revealed that six avirulence genes were detected in the tested strains, with varying occurrence frequencies across different regions. The most prevalent avirulence gene was Avr-pik, whose detection rate was 98.8%, while Avr1-co39 was undetected. Strains from the same region but infecting different host cultivars displayed distinct avirulence gene profiles. Similarly, isolates from different regions infecting the same host cultivar also exhibited differences in avirulence gene composition.

Key words: Magnaporthe oryzae, avirulence gene, occurrence frequency, Zhejiang Province

CLC Number:

S435.111.41

MAO Xueqin, QIU Haiping, CHAI Rongyao, HAO Zhongna, ZHANG Zhen, WANG Jiaoyu, SUN Guocang, WANG Yanli. Analysis of avirulence genes in Magnaporthe oryzae in Zhejiang Province[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2426-2431.

Figures/Tables 5

References 18

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寄主水稻品种	各地区菌株数量						合计
寄主水稻品种	宁波	嘉兴	绍兴	湖州	杭州	温州	合计
宁81	9	0	0	0	0	0	9
宁84	25	0	0	0	0	0	21
宁88	4	0	0	0	0	0	4
宁1268	3	0	0	0	0	0	3
宁1355	2	0	0	0	0	0	2
甬优12	10	0	0	0	0	0	10
甬优15	6	0	0	0	0	0	6
甬优360	4	0	0	0	0	0	4
甬优538	1	0	0	0	0	0	1
甬优720	2	0	0	0	0	0	2
甬优1140	2	0	0	0	0	0	2
甬优1640	3	0	0	0	0	0	3
甬优2640	3	0	0	0	0	0	3
甬优2050	3	0	0	0	0	0	3
嘉禾218	1	1	0	0	0	0	2
嘉58	3	0	0	0	3	0	6
秀水134	0	4	0	0	0	0	4
秀水321	3	11	8	1	0	0	23
绍粳18	6	4	0	0	2	0	12
浙粳88	6	14	0	3	0	0	23
绍糯	3	0	0	0	0	0	3
籼稻	0	0	1	0	0	3	4
粳稻3	0	0	3	0	0	0	3
粳稻2	0	0	1	0	0	0	1
粳稻1	0	0	1	0	0	0	1
闵优香粳	0	1	0	0	0	0	1
天两优616	0	0	0	2	0	0	2
武运粳23号	0	0	0	3	0	0	3
武运粳24号	0	0	0	2	0	0	2
丙13105	3	0	0	0	0	0	3
合计	102	35	14	11	5	3	170

寄主水稻品种	各地区菌株数量						合计
寄主水稻品种	宁波	嘉兴	绍兴	湖州	杭州	温州	合计
宁81	9	0	0	0	0	0	9
宁84	25	0	0	0	0	0	21
宁88	4	0	0	0	0	0	4
宁1268	3	0	0	0	0	0	3
宁1355	2	0	0	0	0	0	2
甬优12	10	0	0	0	0	0	10
甬优15	6	0	0	0	0	0	6
甬优360	4	0	0	0	0	0	4
甬优538	1	0	0	0	0	0	1
甬优720	2	0	0	0	0	0	2
甬优1140	2	0	0	0	0	0	2
甬优1640	3	0	0	0	0	0	3
甬优2640	3	0	0	0	0	0	3
甬优2050	3	0	0	0	0	0	3
嘉禾218	1	1	0	0	0	0	2
嘉58	3	0	0	0	3	0	6
秀水134	0	4	0	0	0	0	4
秀水321	3	11	8	1	0	0	23
绍粳18	6	4	0	0	2	0	12
浙粳88	6	14	0	3	0	0	23
绍糯	3	0	0	0	0	0	3
籼稻	0	0	1	0	0	3	4
粳稻3	0	0	3	0	0	0	3
粳稻2	0	0	1	0	0	0	1
粳稻1	0	0	1	0	0	0	1
闵优香粳	0	1	0	0	0	0	1
天两优616	0	0	0	2	0	0	2
武运粳23号	0	0	0	3	0	0	3
武运粳24号	0	0	0	2	0	0	2
丙13105	3	0	0	0	0	0	3
合计	102	35	14	11	5	3	170

无毒基因	正向引物序列(5'-3')	反向引物序列(5'-3')	片段长度/bp	参考文献
Avr-pita	CGACCCGTTTCCGCCTTTATT	TCCCTCCATTCCAACACTAACG	1 076	[11]
ACE1	CCCAGAGTTGGCGATGATGC	ATGTGGCGGTGACAGAGGAC	661	[11]
Avr-pia	TCTCGGGAAGCTGATTGAGT	CTAACGGCCGGCTAACAAAC	265	[12]
Avr-pit	GAATCGCAGCACTTCCCACT	ATAACTGTCCGCCGCTCGT	287	[12]
Avr1-co39	AATTGCATAATCGCTGCGAT	GTCAAGCTCAGAACTTTGTT	918	[13]
Avr-pik	ACTTTGGGAACTGTCGCTGTC	AGCTGTAACAGGTTCCAGCATC	184	[14]
Avr-pizt	AAACCAGGGCAGCCAAAGA	ATTCCCAATCGAGCCAACG	153	[14]

无毒基因	正向引物序列(5'-3')	反向引物序列(5'-3')	片段长度/bp	参考文献
Avr-pita	CGACCCGTTTCCGCCTTTATT	TCCCTCCATTCCAACACTAACG	1 076	[11]
ACE1	CCCAGAGTTGGCGATGATGC	ATGTGGCGGTGACAGAGGAC	661	[11]
Avr-pia	TCTCGGGAAGCTGATTGAGT	CTAACGGCCGGCTAACAAAC	265	[12]
Avr-pit	GAATCGCAGCACTTCCCACT	ATAACTGTCCGCCGCTCGT	287	[12]
Avr1-co39	AATTGCATAATCGCTGCGAT	GTCAAGCTCAGAACTTTGTT	918	[13]
Avr-pik	ACTTTGGGAACTGTCGCTGTC	AGCTGTAACAGGTTCCAGCATC	184	[14]
Avr-pizt	AAACCAGGGCAGCCAAAGA	ATTCCCAATCGAGCCAACG	153	[14]

菌株来源	菌株总数	无毒基因扩出频率/%
菌株来源	菌株总数	Avr-pik	Avr-pizt	ACE1	Avr-pita	Avr-pia	Avr-pit	Avr1-co39
宁波	102	98.0	99.0	93.1	79.4	47.1	2.0	0
嘉兴	35	100.0	94.3	85.7	85.7	54.3	0	0
绍兴	14	100.0	100.0	92.9	78.6	100.0	7.1	0
湖州	11	100.0	100.0	100.0	72.7	81.8	9.1	0
杭州	5	100.0	100.0	100.0	100.0	60.0	20.0	0
温州	3	100.0	100.0	100.0	66.7	100.0	0	0
合计	170	98.8	98.2	92.4	80.6	56.5	2.9	0