Effect of exogenous hormone on growth and leaf cichoric acid content of double-petal pinecone chrysanthemum (Echinacea purpurea)

doi:10.16178/j.issn.0528-9017.20240634

Abstract

Abstract:

Different concentrations of brassinolide, paclobutrazol, uniconazole, and gibberellin were used for foliar spraying treatment on the Rainbow Rings of double-petaled pinecone chrysanthemum, with water treatment as the control. The effects of four different exogenous hormones on the growth of pinecone chrysanthemum, photosynthetic pigments, and cichoric acid content in leaves were measured and analyzed.The results indicated that hormonal treatment significantly altered various parameters of the Rainbow Rings of idouble-petal pinecone chrysanthemum ncluding plant height, stem diameter, leaf width, leaf length, number of flowers, and cone diameter etc. Appropriate concentration spraying of all four exogenous hormones significantly increased the content of photosynthetic pigments and cichoric acid in the leaves of double-petal pinecone chrysanthemum. Among them, 8 mg·L^-1 brassinolide, 1 000 and 500 mg·L^-1 paclobutrazol were the most effective in promoting chicoric acid content of leaves. Comprehensive analysis revealed that 1 000 mg·L^-1 paclobutrazol had more outstanding and stable effects in increasing the content of active ingredients, promoting the synthesis of photosynthetic pigments, and increasing ornamental value. The findings provide a basis for optimizing the cultivation conditions and enhancing the medicinal value of double-petal pinecone chrysanthemum.

Key words: exogenous hormones, pinecone chrysanthemum, cichoric acid, photosynthetic pigment

CLC Number:

S682.1

GE Yaying, ZHANG Lili, JIN Liang, TIAN Danqing, CAO Qunyang, WAN Xiao. Effect of exogenous hormone on growth and leaf cichoric acid content of double-petal pinecone chrysanthemum (Echinacea purpurea)[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(12): 2933-2938.

Figures/Tables 4

References 8

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处理	激素类型	浓度/(mg·L^-1)
A	芸苔素内酯	2(A1)	4 (A2)	8(A3)
B	多效唑	500(B1)	750(B2)	1 000(B3)
C	烯效唑	10(C1)	30(C2)	45(C3)
D	赤霉素	50(D1)	100(D2)	150(D3)
CK	清水	0(CK)	0(CK)	0(CK)

处理	激素类型	浓度/(mg·L^-1)
A	芸苔素内酯	2(A1)	4 (A2)	8(A3)
B	多效唑	500(B1)	750(B2)	1 000(B3)
C	烯效唑	10(C1)	30(C2)	45(C3)
D	赤霉素	50(D1)	100(D2)	150(D3)
CK	清水	0(CK)	0(CK)	0(CK)

处理	株高/cm	冠幅/cm	茎节数/个	茎粗/mm	叶片长/cm
CK	69.70±4.38 b	55.95±8.86 ab	11.50±1.00 a	5.17±0.78 cde	20.08±2.96 ab
A1	59.13±2.25 de	52.40±4.00 ab	10.75±2.75 ab	4.53±0.21 e	17.75±2.95 b
A2	69.48±2.68 b	57.25±4.41 a	12.00±1.41 a	6.44±0.36 a	18.28±1.67 ab
A3	55.68±2.41 f	53.15±0.95 ab	11.50±1.29 a	5.29±0.81 bcde	17.88±1.07 b
B1	57.60±2.30 ef	53.45±6.60 ab	10.50±0.58 ab	6.24±0.26 ab	17.28±2.83 bc
B2	55.08±1.46 f	53.30±7.83 ab	10.25±2.06 ab	5.94±0.41 abc	19.08±4.89 ab
B3	46.43±2.00 g	51.95±1.54 ab	8.00±1.83 b	5.39±0.65 bcde	13.38±4.04 c
C1	60.75±0.76 de	51.23±7.98 ab	12.25±1.26 a	5.74±0.62 abcd	18.20±2.44 ab
C2	65.93±2.03 c	50.08±6.03 ab	11.75±2.36 a	4.76±1.11 de	17.55±2.51 bc
C3	67.75±1.04 bc	58.85±3.93 a	11.50±1.29 a	5.39±0.35 bcde	22.55±1.61 a
D1	73.75±1.21 a	45.93±3.38 b	12.25±2.63 a	5.12±0.71 cde	21.43±1.20 ab
D2	74.75±1.50 a	51.60±12.28 ab	12.50±1.29 a	4.93±0.54 cde	20.60±1.66 ab
D3	61.35±1.38 d	53.03±8.60 ab	12.00±1.83 a	4.49±0.66 e	19.55±3.47 ab
F	58.76	0.96	1.85	3.88	2.68
P	0	0.51	0.07	0	0.01
处理	叶片宽/cm	花径/cm	花朵数/朵	松果直径/mm
CK	8.87±0.30 ab	12.35±0.94 a	6.25±1.71 c	40.64±6.07 a
A1	7.58±1.97 bc	10.83±1.65 abc	7.25±3.30 c	34.32±4.25 b
A2	9.25±0.65 ab	11.45±1.38 abc	8.50±1.29 bc	36.92±2.40 ab
A3	8.15±1.68 abc	10.15±0.97 bc	8.75±1.50 bc	32.85±2.95 b
B1	8.05±1.20 abc	12.23±1.17 ab	9.75±1.26 bc	35.46±2.86 ab
B2	6.68±0.39 c	11.23±0.26 abc	9.75±1.71 bc	32.57±4.14 b
B3	6.51±1.15 c	11.13±1.25 abc	14.00±6.00 a	31.31±0.97 b
C1	9.05±0.62 ab	9.80±1.93 c	10.50±2.38 abc	31.30±6.20 b
C2	8.43±1.18 abc	10.85±1.35 abc	12.00±0.82 ab	32.77±2.51 b
C3	9.73±1.59 a	11.35±1.64 abc	7.75±3.86 bc	32.87±3.58 b
D1	8.83±1.63 ab	10.73±1.39 abc	8.25±1.26 bc	31.61±5.31 b
D2	8.10±0.80 abc	9.70±0.29 c	7.25±1.71 c	31.76±3.00 b
D3	6.78±1.25 c	10.50±1.25 abc	8.50±1.91 bc	31.13±3.06 b
F	2.83	1.61	2.65	1.98
P	0.01	0.13	0.01	0.05

处理	株高/cm	冠幅/cm	茎节数/个	茎粗/mm	叶片长/cm
CK	69.70±4.38 b	55.95±8.86 ab	11.50±1.00 a	5.17±0.78 cde	20.08±2.96 ab
A1	59.13±2.25 de	52.40±4.00 ab	10.75±2.75 ab	4.53±0.21 e	17.75±2.95 b
A2	69.48±2.68 b	57.25±4.41 a	12.00±1.41 a	6.44±0.36 a	18.28±1.67 ab
A3	55.68±2.41 f	53.15±0.95 ab	11.50±1.29 a	5.29±0.81 bcde	17.88±1.07 b
B1	57.60±2.30 ef	53.45±6.60 ab	10.50±0.58 ab	6.24±0.26 ab	17.28±2.83 bc
B2	55.08±1.46 f	53.30±7.83 ab	10.25±2.06 ab	5.94±0.41 abc	19.08±4.89 ab
B3	46.43±2.00 g	51.95±1.54 ab	8.00±1.83 b	5.39±0.65 bcde	13.38±4.04 c
C1	60.75±0.76 de	51.23±7.98 ab	12.25±1.26 a	5.74±0.62 abcd	18.20±2.44 ab
C2	65.93±2.03 c	50.08±6.03 ab	11.75±2.36 a	4.76±1.11 de	17.55±2.51 bc
C3	67.75±1.04 bc	58.85±3.93 a	11.50±1.29 a	5.39±0.35 bcde	22.55±1.61 a
D1	73.75±1.21 a	45.93±3.38 b	12.25±2.63 a	5.12±0.71 cde	21.43±1.20 ab
D2	74.75±1.50 a	51.60±12.28 ab	12.50±1.29 a	4.93±0.54 cde	20.60±1.66 ab
D3	61.35±1.38 d	53.03±8.60 ab	12.00±1.83 a	4.49±0.66 e	19.55±3.47 ab
F	58.76	0.96	1.85	3.88	2.68
P	0	0.51	0.07	0	0.01
处理	叶片宽/cm	花径/cm	花朵数/朵	松果直径/mm
CK	8.87±0.30 ab	12.35±0.94 a	6.25±1.71 c	40.64±6.07 a
A1	7.58±1.97 bc	10.83±1.65 abc	7.25±3.30 c	34.32±4.25 b
A2	9.25±0.65 ab	11.45±1.38 abc	8.50±1.29 bc	36.92±2.40 ab
A3	8.15±1.68 abc	10.15±0.97 bc	8.75±1.50 bc	32.85±2.95 b
B1	8.05±1.20 abc	12.23±1.17 ab	9.75±1.26 bc	35.46±2.86 ab
B2	6.68±0.39 c	11.23±0.26 abc	9.75±1.71 bc	32.57±4.14 b
B3	6.51±1.15 c	11.13±1.25 abc	14.00±6.00 a	31.31±0.97 b
C1	9.05±0.62 ab	9.80±1.93 c	10.50±2.38 abc	31.30±6.20 b
C2	8.43±1.18 abc	10.85±1.35 abc	12.00±0.82 ab	32.77±2.51 b
C3	9.73±1.59 a	11.35±1.64 abc	7.75±3.86 bc	32.87±3.58 b
D1	8.83±1.63 ab	10.73±1.39 abc	8.25±1.26 bc	31.61±5.31 b
D2	8.10±0.80 abc	9.70±0.29 c	7.25±1.71 c	31.76±3.00 b
D3	6.78±1.25 c	10.50±1.25 abc	8.50±1.91 bc	31.13±3.06 b
F	2.83	1.61	2.65	1.98
P	0.01	0.13	0.01	0.05

处理	叶绿素a含量	叶绿素b含量	叶绿素含量	类胡萝卜素含量
CK	0.34±0.02 b	0.15±0.01 de	0.49±0.03 c	0.06±0 c
A1	0.40±0.01 a	0.21±0.01 ab	0.61±0.02 ab	0.07±0 b
A2	0.32±0.01 bc	0.16±0.01 de	0.48±0.01 c	0.06±0 c
A3	0.23±0.03 d	0.14±0.02 de	0.37±0.04 d	0.04±0 d
B1	0.43±0.02 a	0.23±0.02 a	0.67±0.04 a	0.08±0 a
B2	0.30±0.01 bc	0.15±0.01 de	0.45±0.02 c	0.06±0 c
B3	0.42±0.02 a	0.21±0.01 ab	0.63±0.03 ab	0.08±0 ab
C1	0.12±0.01 e	0.07±0.01 f	0.19±0.02 e	0.03±0 e
C2	0.27±0.07 cd	0.15±0.05 de	0.42±0.11 cd	0.05±0.01 cd
C3	0.29±0.03 bc	0.14±0.01 e	0.43±0.05 cd	0.06±0.01 c
D1	0.29±0.01 bc	0.14±0 e	0.42±0.01 cd	0.05±0 c
D2	0.32±0.01 b	0.17±0.01 cd	0.49±0.02 c	0.06±0 c
D3	0.39±0.01 a	0.20±0.01 bc	0.58±0.02 b	0.07±0 b
F	33.31	19.34	27.30	25.11
P	0	0	0	0