Archive

11 March 2025, Volume 66 Issue 3 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

CONTENTS 2025, 66 (3):  0-0.  Abstract ( 58 )   PDF (47682KB) ( 105 )   Related Articles | Metrics

Select

Study on the benefit-sharing mechanism of geographical indication agricultural products—taking Kaihua Qingshui fish as an example MIAO Miao, LIU Shuyue 2025, 66 (3):  541-545.  DOI: 10.16178/j.issn.0528-9017.20250097 Abstract ( 70 )   HTML ( 13 )   PDF (1428KB) ( 123 )   Geographical indication agricultural products refer to product categories that are produced in specific regions and can reflect the natural ecological characteristics and cultural development factors of that region. Based on the diverse value attributes and benefit sharing mechanism of geographical indication agricultural products, this article systematically summarizes the difficulties faced by the development of the Kaihua Qingshui fish industry, and proposes a benefit sharing path for geographical indication agricultural products using Kaihua Qingshui fish as an example from the aspects of strengthening top-level design, enhancing ecological feedback, improving regulatory mechanisms, and enhancing cultural identity. The aim is to provide useful references for research in related fields and sustainable development of the industry. Figures and Tables | References | Related Articles | Metrics

Select

Study on the benefit-sharing model of utilizing brittle culm rice to promote the development of the mountain spring water fish farming system in Kaihua of Zhejiang SHI Lingli, WANG Yin 2025, 66 (3):  546-550.  DOI: 10.16178/j.issn.0528-9017.20250100 Abstract ( 40 )   HTML ( 4 )   PDF (1175KB) ( 122 )   In this study, the creation of brittle culm rice varieties and the development of mountain spring water fish farming system in Kaihua County, Zhejiang Province were used as the breakthrough point to explore the benefit sharing model between innovative germplasm resources development and traditional farming methods. A resource sharing model based on the integration of agriculture and fishery was proposed by analyzing the ecological value and synergistic development potential of the creation of brittle culm rice varieties and mountain spring water fish culture system. It is expected to provide scientific basis and theoretical reference for the integrated development of agriculture and fishery, promote the sustainable development of regional agriculture and ecological aquaculture industry, and provide a model for the innovative utilization of traditional agricultural resources and the implementation of rural revitalization strategy. References | Related Articles | Metrics

Select

Iegitimacy and implementation path of farmers' participation in the benefit-sharing of germplasm resources LI Lingling, DENG Ziyuan 2025, 66 (3):  551-556.  DOI: 10.16178/j.issn.0528-9017.20250006 Abstract ( 31 )   HTML ( 2 )   PDF (1283KB) ( 111 )   Modern breeding techniques completely separate seeds from the value of their genes, making them a commodity beyond the control of farmers. However, based on the public nature of germplasm resources, the ownership basis of state and collective ownership, and the labor contribution of farmers as germplasm resources conservators, providers and participants in plant breeding, farmers should be recognized as legitimate to participate in the right to benefit sharing in modern breeding systems. Therefore, the nature of farmers' right to participate in benefit-sharing can be clarified at the institutional level, and the content, mode and proportion of benefit-sharing can be clarified, as well as the procedures and remedies for participating in benefit-sharing. Strengthen the capacity building of farmers to participate in breeding and germplasm resource utilization at the practical level, and release the functions and values of government, breeding companies and farmers in breeding to increase the output of benefits. In order to ensure biodiversity and food security in China, it provides the ability support and system guarantee for farmers to participate in the benefit sharing of germplasm resources, and encourages farmers to participate in the protection of germplasm resources. Figures and Tables | References | Related Articles | Metrics

Select

Study on the benefit-sharing mechanism of traditional Chinese medicine industry in mountainous areas—taking Kaihua County as an example YU Yangmei, LI Haojie, MIAO Miao 2025, 66 (3):  557-561.  DOI: 10.16178/j.issn.0528-9017.20250098 Abstract ( 33 )   HTML ( 3 )   PDF (1302KB) ( 103 )   The development of traditional Chinese medicine industry in mountainous areas is of great significance for excavating and protecting authentic traditional Chinese medicine germplasm resources, promoting farmers' income increase, and narrowing the income gap between urban and rural areas. This article takes Kaihua County as an example to analyze the logical mechanism of the benefits of various parties in the traditional Chinese medicine industry, systematically sort out the current situation and difficulties of industry development, and propose a path and mechanism to promote the sharing of benefits in the traditional Chinese medicine industry in mountainous areas from the aspects of seed source determination, value transformation, and factor allocation, in order to explore a path of leading common prosperity through industrial revitalization. Figures and Tables | References | Related Articles | Metrics

Select

Study on the pathways to benefit-sharing in alpine vegetables industry—taking the the alpine pepper industry in Kaihua County as an example WU Yufeng, LIU Shuyue, SHI Lingli 2025, 66 (3):  562-565.  DOI: 10.16178/j.issn.0528-9017.20250088 Abstract ( 34 )   HTML ( 2 )   PDF (1142KB) ( 107 )   As a kind of ecological product, alpine vegetables not only show high quality product characteristics, but also contain significant ecological service value by virtue of the natural advantages given by its unique growing environment. Taking the alpine pepper industry in Kaihua County as an example, this paper explains the internal logic of benefit-sharing in the alpine vegetable industry, deeply identifies the main problems existing in the process of industrial development, and puts forward the realization path of benefit-sharing from the aspects of improving the germplasm resource evaluation system, promoting the integrated development of industries, strengthening the support force of scientific and technological innovation, and improving the industrial benefit-sharing mechanism. It provides theoretical and practical basis for the sustainable development of alpine vegetable industry. References | Related Articles | Metrics

Select

Effects of perishable waste organic fertilizer application on rice growth and soil physicochemical properties CAI Daming, LU Xin, LI Penghao, WANG Weiping, ZHOU Zhiguo, HONG Chunlai 2025, 66 (3):  566-571.  DOI: 10.16178/j.issn.0528-9017.20240121 Abstract ( 47 )   HTML ( 13 )   PDF (2539KB) ( 132 )   The study on the effects of perishable waste organic fertilizer on rice yield, growth and soil physicochemical properties was beneficial to the fertilizer utilization of perishable waste. The field experiments showed that the yield of rice in T1 treatment was higher than that of CK1 and CK2. When the amount of perishable waste organic fertilizer used in 667 m2 reached 2 500 kg, the rice yield decreased significantly. With the increase of perishable waste organic fertilizer application amount, soil pH value and organic matter content showed an upward trend. Compared with CK1 and CK2, the contents of alkali-hydrolyzable nitrogen and available phosphorus in T1 treatment were slightly lower, but the available nutrient content in T2 treatment was significantly higher. There was no significant difference between the total soluble salt content of soil in T1 treatment and CK2, and with the further increase of perishable waste organic fertilizer application amount, the contents of total soluble salt, sodium ion and chloride ion showed an obvious upward trend. The application of perishable waste organic fertilizer caused a decrease on the oil content of the soil. Overall, 1 000 kg was the optimal amount for perishable waste organic fertilizer in 667 m2 paddy field. This study provides a scientific basis for the utilization of perishable waste as fertilizer. Figures and Tables | References | Related Articles | Metrics

Select

Effect of slow release fertilizer on double cropping rice yield and fertilizer utilization rate in central Zhejiang SHENG Yanfei, ZHANG Wenhan, FU Liqing, HUANG Qiying, LU Zhongcheng, YE Nan, LU Jiujin 2025, 66 (3):  572-575.  DOI: 10.16178/j.issn.0528-9017.20240722 Abstract ( 39 )   HTML ( 8 )   PDF (1153KB) ( 121 )   In order to investigate the effects of slow-release fertilizer on double-cropping rice yield and fertilizer utilization rate in central Zhejiang, a field trial was conducted in 2022, and 8 treatments were set. In the early rice season, the grain yields of ZNK, ZNP and ZHLG treatments increased by 45.42%, 43.59% and 70.33%, respectively, compared with ZPK treatment. The fertilizer utilization efficiency of nitrogen, phosphorus and potassium in ZHLG treatment was 40.94%, 27.10% and 72.50%, respectively. In the late rice season, the grain yield of 667 m2 of rice treated with WHLG was the highest, which was 536.3 kg, 6.81% higher than that of WPK treatment. The fertilizer utilization efficiency of nitrogen, phosphorus and potassium in WHLG treatment was 41.00%, 21.50% and 73.50%, respectively. In conclusion, the application of slow-release fertilizer in the production process of double cropping rice in central Zhejiang can effectively improve rice yield and nitrogen fertilizer use rate, which is a more scientific and reasonable fertilization method. Figures and Tables | References | Related Articles | Metrics

Select

Effect of nano-selenium fertilizer on rice yield and selenium content ZHENG Lin, YANG Shuai, HUANG Xin, ZHENG Lei, JIANG Yunzhu, DAI Fen 2025, 66 (3):  576-579.  DOI: 10.16178/j.issn.0528-9017.20240109 Abstract ( 40 )   HTML ( 6 )   PDF (1273KB) ( 104 )   Selenium is one of the essential trace elements for the human body and has important health benefits. Different fertilization methods and concentrations were used to study the effects of nano-selenium fertilizer on selenium content and yield of rice variety Hanyou 73. The results showed that 150 mL of nano-selenium fertilizer applied to 667 m2 roots could effectively increase the selenium content of rice tissues, and the selenium content in leaves changed most significantly. In rice grains, the selenium content was in the order of T2>T1>T3>T4, that was, T2 treatment had the best effect, and the absorption of selenium in rice would be inhibited with excessive application of nano-selenium fertilizer. T2 treatment could effectively promote the yield of rice, which was 17.49% higher than that of CK. One year after rice planting, the selenium content in the soil treated with T2 decreased slightly, and the selenium content in the other treated soils increased to varying degrees. In this paper, we compared the selenium absorption capacity of rice under different nano-selenium fertilizer fertilization methods and concentrations, and increased yield and efficiency to produce high-quality selenium-rich rice. Figures and Tables | References | Related Articles | Metrics

Select

Effects of biochar and organic fertilizer on soil properties and rice growth MA Liang, JIN Xinmei, ZHANG Weixing, FANG Yunfeng 2025, 66 (3):  580-584.  DOI: 10.16178/j.issn.0528-9017.20231186 Abstract ( 41 )   HTML ( 5 )   PDF (1259KB) ( 118 )   In order to explore the effects of biochar and organic fertilizer on soil fertility and rice growth, Jia 67 was used as the test crop and five treatments were set up to study the changes of soil physical and chemical properties, rice yield and its constituent factors. The results showed that compared with the control, the soil bulk density decreased under the combined application of biochar and organic fertilizer. The content of soil organic matter increased by 13.26% under MC0.5F1 treatment. The soil pH value was the highest under SC0.5F1 treatment. The rice yield increased by 5.58%-7.48% compared with the control after the combined application of biochar and organic fertilizer. The correlation analysis showed that the combined application of biochar and organic fertilizer mainly increased the effective panicle number of rice by increasing soil pH value, organic matter, total nitrogen and total potassium content, thereby increasing rice yield. Therefore, the combined application of biochar and organic fertilizer can improve soil physical and chemical properties and increase rice yield. Figures and Tables | References | Related Articles | Metrics

Select

Effect of the combined application of polyurethane-coated and uncoated urea at different stages on rice yield and nitrogen fertilizer efficiency SHEN Wenying, CHEN Weilong, XIAO Jiali, TU Changpeng 2025, 66 (3):  585-589.  DOI: 10.16178/j.issn.0528-9017.20240593 Abstract ( 31 )   HTML ( 2 )   PDF (1212KB) ( 112 )   Field experiment method was used in this study, and five treatment methods were set up: a control group with no fertilizer application (CK), application of only phosphorus and potassium fertilizers (PK), application of conventional nitrogen, phosphorus, and potassium fertilizers (NPK), a combination of 80% controlled-release fertilizer and 20% conventional urea as base fertilizer (N2PK), and 80% controlled-release fertilizer as base fertilizer and 20% conventional urea as topdressing (N3PK). The study aimed to investigate the effects of controlled-release urea and conventional urea applied at different stages on rice yield and nitrogen fertilizer efficiency. The results showed that compared with the control group (CK) with no fertilizer, the PK treatment significantly promoted the growth of rice grain and straw, increasing yields by 25.7% and 11.8%, respectively. Grain and straw yields under nitrogen application treatments increased by 66.4% to 79.1% and 38.4% to 79.2%, respectively. Compared with NPK, the grain yields of N2PK and N3PK increased by 707 kg·hm-2 and 562 kg·hm-2, and straw yields increased by 29.5% and 18.4%, respectively. The yield-increasing effect of the N2PK treatment was more obvious. Although there were no significant differences in grain nitrogen content among the nitrogen treatments, the straw nitrogen content in N2PK and N3PK was significantly higher by 15.6% and 11.4%, respectively, compared with NPK. The nitrogen uptake by grains in N2PK increased significantly by 16.1%, and the nitrogen uptake by straw in N2PK and N3PK increased significantly by 49.7% and 31.8%, respectively. Nitrogen fertilizer application caused nitrogen accumulation in the soil, especially in the NPK treatment, which had a much higher nitrogen surplus than the controlled-release fertilizer treatments. The apparent utilization efficiency of N2PK and N3PK was higher by 21.9 and 12.5 percentage points, respectively, compared with NPK. Therefore, applying 80% nitrogen from controlled-release urea and 20% from conventional urea as base fertilizer is an optimal fertilization method, effectively improving rice yield. Figures and Tables | References | Related Articles | Metrics

Select

Effects of slow and controlled release fertilizers combined with straw returning on rice yield and nitrogen use efficiency ZHAO Juntao, SHI Yanping, CAO Mengjiao, REN Jiajia, WANG Yeqing, CHEN Yiping, WANG Yirong, CHEN Gui 2025, 66 (3):  590-594.  DOI: 10.16178/j.issn.0528-9017.20240745 Abstract ( 37 )   HTML ( 4 )   PDF (1179KB) ( 116 )   Field experiments were conducted to compare the effects of slow and controlled release fertilizer combined with straw returning on rice yield and nitrogen use efficiency. The results showed that the use of slow and controlled release fertilizer and urea-ammonium fertilizer to replace traditional compound fertilizer and urea, respectively, and straw returning to the field at the same time could improve rice yield and dry matter accumulation. The fertilization mode of slow and controlled release fertilizer + urea-ammonium fertilizer + straw returning had an effect on the improvement of nitrogen accumulation and nitrogen apparent efficiency. Under relatively reasonable nitrogen application conditions, the continued application of nitrogen fertilizer led to a decrease in crop yield and a decrease in the nitrogen apparent efficiency. In actual production, the fertilization mode of basic application of slow and controlled release fertilizer, top-application of urea-ammonium and straw returning can be promoted. However, in order to improve the nitrogen fertilizer use efficiency and reduce the loss of chemical fertilizer, the total nitrogen input of 667 m2 chemical fertilizer should not be less than 12 kg, and 12-15 kg should be appropriate, so as to achieve high yield and efficient utilization of resources in late rice. Figures and Tables | References | Related Articles | Metrics

Select

Effects of different seedling cultivation methods on main agronomic traits and yield of winemaking sorghum ZHANG Lin, HU Huaizhou, ZHANG Xulin, YIN Yong, ZHANG Deyin, YING Heng, PENG Yao, ZHOU Junhui 2025, 66 (3):  595-599.  DOI: 10.16178/j.issn.0528-9017.20231206 Abstract ( 34 )   HTML ( 5 )   PDF (1172KB) ( 116 )   In order to clarify the effects of different seedling cultivation methods on the main agronomic traits and yield of winemaking sorghum, the sorghum variety Yinuohong 8 was selected as the experimental material, and the development process, traits and yield components of sorghum seedlings were compared and analyzed from 2022 to 2023. The results showed that the root number of seedlings in sorghum was higher in traditional sowing method than that of other seedling cultivation methods. Under the floating seedling cultivation method, the hundred-seedling weight and seedling emergence rate of sorghum were higher than those of other treatments. The traditional sowing method extended the sowing-transplanting period and the whole growth period, shortened the period from transplanting to heading stage, and had the lowest yield. The floating seedling cultivation method can achieve high yield by increasing the panicle weight. There was a significantly negative correlation between sorghum yield and the number of days in the sowing-transplanting period, and the correlation coefficient was -0.93. Therefore, combined with the production needs of sorghum in various places and according to local conditions, it is recommended to use floating seedling cultivation method for the best effect. Figures and Tables | References | Related Articles | Metrics

Select

Effect of defoliation and ripening time on cotton yield and quality ZHAO Zhongting, FAN Haichao, ZHANG Zhiheng, ZHANG Guihua 2025, 66 (3):  600-603.  DOI: 10.16178/j.issn.0528-9017.20240096 Abstract ( 34 )   HTML ( 4 )   PDF (1141KB) ( 110 )   In this study, the effects of different defoliation and ripening time on cotton yield and quality were studied using hybrid cotton variety Ruiza 818 as the material. The results showed that defoliation and ripening had a great effect on the single boll weight of cotton, and defoliation and ripening affected the later growth and development of the upper boll. The yield of pre-frost seed cotton in the plot after defoliation and ripening was significantly higher than that of the control, and the yield of seed cotton after frost was significantly lower than that of the control, and the total yield was slightly higher than that of the control. Early defoliation and ripening reduced the lint percentage, fiber length, breaking strength and micronaire value of cotton. However, with the delay of defoliation and ripening time, the lint percentage, fiber length, breaking strength and micronaire value of cotton were not significantly different from those of the control. Figures and Tables | References | Related Articles | Metrics

Select

Effects of different disease grades of sesame stem rot on individual plant yield and traits ZHENG Lei, ZHANG Xianmei, LIU Meiru, WEI Songshan, WU Hemin, SONG Gaojie, SUN Hao 2025, 66 (3):  604-608.  DOI: 10.16178/j.issn.0528-9017.20240595 Abstract ( 29 )   HTML ( 4 )   PDF (1181KB) ( 111 )   In order to investigate the impact of different disease grades of sesame stem rot on individual plant yield and traits, this study compared paired data between diseased plants and control plants under natural disease comditions. The results showed that the first grade diseased plant had a 2.09% reduction in production compared with the control, the second grade reduced production by 21.76%, the third grade reduced production by 23.28%, and the fourth grade reduced production by 67.49%. There was a highly significant correlation between the yield decline rate of all diseased plants and the severity of the disease. As the grades of sesame stem rot increased, the rate of yield loss per plant, capsule number reduction rate, effective capsule reduction rate, thousand grain weight reduction rate, plant height reduction rate, and fruit node number reduction rate increased. For these six traits, there was no significant difference between the first grade diseased plant and the control. The difference between the fourth grade diseased plant and the control was extremely significant and the decrease rate of the above traits of the first grade diseased plant and that of the fourth grade diseased plant all reached significant or extremely significant differences. The decrease in capsule number plays a decisive role in the reduction of yield per plant. The decrease in thousand grain weight caused by smaller grains may play an important role in the decrease of yield per plant at specific disease grades. This study provides a basic condition for estimating population yield. Figures and Tables | References | Related Articles | Metrics

Select

Effect of the nano-carbon foliar fertilizer on tobacco quality in field LUO Zhenbao, WANG Wenkai, ZHAO Erwei, ZHAI Xu, YU Song, CHEN Dan, ZHANG Long, Yang Xiaoe 2025, 66 (3):  609-614.  DOI: 10.16178/j.issn.0528-9017.20231108 Abstract ( 39 )   HTML ( 5 )   PDF (2578KB) ( 107 )   In order to determine the optimal application period of nano-foliar fertilizer in tobacco growth and its role, a field experiment was conducted in Chengjiang tobacco area of Yuxi, Yunnan Province to study the effects of nano-carbon foliar fertilizer (NCF) on tobacco quality at different periods. NCF spraying on leaves significantly increased the total nitrogen content of tobacco leaves. The average increase of middle and upper tobacco leaves was 7.37% and 14.45% respectively. Reducing sugar decreased by 17.41% on average. The nicotine content in middle and upper tobacco leaves decreased by 23.84% and 33.87% respectively. In addition, the potassium content of central tobacco leaves was significantly increased by 12.50% on average when NCF was applied in different periods. Among the four spraying periods, T1 treatment (spraying three times in the group growth period, flourishing period and peak period) had the greatest impact on the quality of tobacco. In conclusion, after spraying NCF on the leaf surface, the tobacco can improve nitrogen, increase potassium and reduce nicotine in different degrees. Figures and Tables | References | Related Articles | Metrics

Select

Stability and regionalization of nicotine, total nitrogen and nitrogen-nicotine ratio in Chuxiong K326 tobacco leaves GENG Shaowu, DING Yishu, CHAI Yunxia, LU Yao, LIN Yunhong, BU Yunhong, FU Yanyan, AI Kebiao, SUN Jiaxian, ZHANG Wangong, LI Xiaoyan, ZHANG Shengyu 2025, 66 (3):  615-622.  DOI: 10.16178/j.issn.0528-9017.20231144 Abstract ( 30 )   HTML ( 1 )   PDF (1245KB) ( 103 )   In view of the chemical quality indexes such as nicotine, total nitrogen content and nitrogen-nicotine ratio that affect the quality of tobacco leaves of the main variety K326 in Chuxiong tobacco-growing area, the stability analysis and the regional study of villages and towns were carried out. The results showed that the absolute stability of nicotine and nitrogen-nicotine ratio of K326 in Chuxiong tobacco area was at a moderate level. The relative stability of nicotine content was generally good in the upper leaves, fair to good in the middle leaves, and good in the lower leaves. The content of nicotine in the upper and middle leaves was lower than that of the quality target of high-quality tobacco leaves, which was 42.80% and 46.68%, respectively. The relative stability of nitrogen-nicotine ratio in upper and middle leaves was generally fair, while that in lower leaves was poor. The nitrogen-nicotine ratios of upper, middle and lower leaves were 57.85%, 48.47% and 66.10%, respectively, which were higher than the quality requirements of high-quality tobacco leaves. The absolute stability variation of total nitrogen content was small. Reducing the nitrogen-nicotine ratios by increasing nicotine content is the key way to regulate the coordination of chemical components of K326 tobacco leaves in Chuxiong tobacco-growing areas. The main production techniques to increase nicotine content include appropriate increase of nitrogen fertilizer, appropriate early topping, reduction of topping height and less leaf retention. It is also expected to make breakthroughs and apply them to production as soon as possible by directional improvement of K326 new lines with high nicotine. Figures and Tables | References | Related Articles | Metrics

Select

Phylogenetic analysis of Panax japonicus (TianMu) and its relatives based on DNA barcode sequences XIAO Wenfei, YAN Jianli, XIN Ya, CHAI Weiguo 2025, 66 (3):  623-626.  DOI: 10.16178/j.issn.0528-9017.20240913 Abstract ( 26 )   HTML ( 1 )   PDF (1274KB) ( 107 )   To explore the evolutionary status of Panax japonicus (TianMu), DNA barcode markers including ITS1/ITS2, ITS2, psbA-trnH, and rbcL were amplified using PCR from Panax japonicus (TianMu) samples. Additionally, the homologous sequences of 8 Panax species and 3 exogenous plants were downloaded from NCBI. A phylogenetic tree was constructed using MEGA 11.0 software to analyze the phylogenetic relationships of P. japonicus (TianMu) and its closely related species at the molecular level. The results indicated that the ITS1/ITS2 sequences showed significant differences between species but were conserved within species; ITS2 sequences had the richest polymorphism and also exhibited intraspecific variation. The psbA-trnH and rbcL sequences were not effective in distinguishing some closely related species. A phylogenetic tree was constructed using the ITS1/ITS2 sequences with the neighbor joining method, which showed that Panax. japonicus (TianMu) was closely related to Panax ginseng and Panax quinquefolius, and they were classified into the same group; the differences between Panax. japonicus (TianMu) and Panax notoginseng and Panax japonicus (Enshi) were greater than the minimum genetic differences within species. The genetic distances between the above-mentioned Panax species ranged from 0.003 to 0.033, indicating that these Panax species are closely related. This study is significant for the protection and exploration of genetic resources of Panax japonicus (TianMu). Figures and Tables | References | Related Articles | Metrics

Select

Comparative experiment on greenhouse cultivation and wild cultivation of Ganoderma lucidum FENG Qing 2025, 66 (3):  627-632.  DOI: 10.16178/j.issn.0528-9017.20231080 Abstract ( 29 )   HTML ( 2 )   PDF (1199KB) ( 100 )   Basswood Ganoderma lucidum and bag Ganoderma lucidum were used as materials to carry out greenhouse cultivation and understory imitation wild cultivation management. The effects of cultivation methods and materials on the growth cycle, fruiting body agronomic traits and nutrient content of Ganoderma lucidum were compared, and the effects of harvest time during the maturity period of Ganoderma lucidum on the nutrient composition of fruiting body and spore powder cultivated in greenhouses were discussed. The results showed that the understory imitation wild cultivated Ganoderma lucidum bud earlier, but the total growth cycle was longer than that of the greenhouse cultivation. Under the same cultivation mode, the agronomic traits of basswood Ganoderma lucidum were better than those of bag material. With the same cultivation materials, the contents of polysaccharides, triterpenoids and sterols in the understory imitation wild cultivated Ganoderma lucidum were higher. The content of polysaccharides in fruiting bodies harvested at the early stage was the highest, and then decreased slowly, while the content of triterpenes and sterols was the opposite. The spore powder collected in the early stage had the highest content of polysaccharides, triterpenoids and sterols. This experiment preliminarily provided a scientific basis for the optimization of cultivation materials, cultivation methods and harvesting time of Ganoderma lucidum. Figures and Tables | References | Related Articles | Metrics

Select

Cultivation techniques of quinoa seedlings YAO Liwu, WU Yingqi, WANG Yanbin, LIAN Jianjun, WANG Changmei 2025, 66 (3):  633-640.  DOI: 10.16178/j.issn.0528-9017.20231199 Abstract ( 22 )   HTML ( 1 )   PDF (1733KB) ( 104 )   In order to explore the cultivation methods of quinoa seedlings in southwest Zhejiang, Qingli 1 and Hongli were used as experimental materials to carry out comparative experiments on sowing date, cultivation mode and seed dosage. The results showed that quinoa seedlings could be cultivated with a seed rate of 1.5 g·m-2, and the sowing date after July should be Hongli. High temperature is the main limiting factor for the growth of quinoa seedlings in the local area, and quinoa seedlings prefer moist habitats, but heavy rainfall leads to root rot and dead seedlings, so it is advisable to choose a suitable cultivation mode according to the temperature and rainfall at the sowing date. Quinoa seedlings do not have high requirements for sunlight, but the facility cultivation mode is not suitable for shading and cooling. The main pest of quinoa seedlings is Spodoptera litura, which damages the stems and leaves of seedlings with larvae, which occurs from late June to September. Quinoa seedlings are prone to cataplexy in high temperature and high humidity seasons, which seriously affects the yield and quality of seedlings. Figures and Tables | References | Related Articles | Metrics

Select

Effects of different dosages of microbial fertilizer on broccoli yield and soil nutrient content ZHANG Huaijie, HU Tiejun 2025, 66 (3):  641-644.  DOI: 10.16178/j.issn.0528-9017.20231197 Abstract ( 30 )   HTML ( 4 )   PDF (1159KB) ( 107 )   In order to improve the fertilization technology of broccoli, field experiments were carried out to study the effects of different dosages of microbial fertilizer on the economic traits, fertilizer use efficiency and soil nutrients of broccoli. The results showed that the application of microbial fertilizer could increase the yield of broccoli, the absorption of nitrogen, phosphorus and potassium and the fertilizer use efficiency, and the content of soil nutrients. The F4 treatment (normal fertilization+microbial fertilizer 1 500 kg·hm-2) has the best comprehensive performance and can be popularized and applied in agricultural production. Figures and Tables | References | Related Articles | Metrics

Select

Effects of different full-biodegradable mulching films on greenhouse fresh peanut yield XU Chuchu, LI Kunyu, WANG Huaying, SUN Shuni, SHEN Diwen, LI Feng, WU Bin, WANG Huafeng 2025, 66 (3):  645-651.  DOI: 10.16178/j.issn.0528-9017.20240570 Abstract ( 29 )   HTML ( 3 )   PDF (1212KB) ( 111 )   Nine kinds of full-biodegradable mulching films were selected for the cultivation of facility fresh peanuts in early spring in Hangzhou City. The yield analysis showed that the full-biodegradable mulching films increased the yield significantly compared with the bare soil cultivation (control), and the yield of 10 μm black film was the most prominent, reaching 950.16 kg per 667 m2, with an increase of 61.6% compared with the control. The production of 8 μm white film was stable in the range of 857.81-880.90 kg per 667 m2, and the increase rate was 45.9%-49.8%. The yield of 6 μm white film and 10 μm films in other colors increased 28.1% on average. The economic benefit analysis showed that the profit of 10 μm black film reached 10 852.6 yuan per 667 m2, and the profit of 8 μm white film reached 9 781.8 yuan. Environment investigation showed that there was no need to remove and recycle films after harvesting, so there was no residual pollution problem of mulching film. In conclusion, it is recommended to use 10 μm black and 8 μm white full-biodegradable mulching film for the cultivation of greenhouse fresh peanuts in Hangzhou in early spring. Figures and Tables | References | Related Articles | Metrics

Select

Introduction performance and high-quality and efficient cultivation technology of Dongkui bayberry in Huaping County FENG Zhansheng, LIANG Senmiao 2025, 66 (3):  652-657.  DOI: 10.16178/j.issn.0528-9017.20240542 Abstract ( 31 )   HTML ( 1 )   PDF (1197KB) ( 104 )   From 2021, 2.67 hm2 of Dongkui bayberry was planted in Huaping County, and its introduction performance was observed for three consecutive years. The results showed that the growth period of sprouting, flowering and fruiting in Huaping County was basically stable, and the local climate and environment were conducive to the normal flowering and fruiting of bayberry. In 2022, the leaf area and chlorophyll content were the best, and the average fruit weight was also the highest, reaching 22.78 g. In 2022 and 2023, the soluble solids content were better, reaching 14.20% and 14.50%, respectively, and the ratio of sugar and acid were better, reaching 15.20 and 15.09, respectively. The output value in 2021, 2022 and 2023 were 273 200, 272 800 and 268 900 yuan per hectare, respectively. Therefore, Dongkui bayberry tree in Huaping County is strong and resistant, with early maturity, large fruit shape, stable yield, good quality, high and stable sales price, and good output value. On the basis of high-quality and efficient cultivation, through plastic pruning, fertilizer and water management, shoot control and flower promotion, fruit thinning and yield control, pest control and other technical measures, it provides production reference for further expansion and development. Figures and Tables | References | Related Articles | Metrics

Select

Effect of slope planting on the growth and fruiting of Dongkui bayberry WANG Yang, MOU Wangbin, LIANG Senmiao 2025, 66 (3):  658-661.  DOI: 10.16178/j.issn.0528-9017.20240543 Abstract ( 29 )   HTML ( 1 )   PDF (1173KB) ( 100 )   In order to explore the effects of different slope planting on the growth and fruiting of Dongkui bayberry, the 15°, 28° and 43° slopes were selected on the 650 m altitude mountain in 2017. The results showed that the trunk thickness and tree height of 7-year-old bayberry planted on 15° slope increased by 8.12%-8.47% and 25.08%-29.24% respectively compared with those planted on 28° and 43° slopes, respectively. The 6-7-year-old bayberry grew faster and the crown diameter expanded faster when planted on 15° slope, while the trunk thickness increased relatively quicker when planted on 43° slope. The maturity period of 7-year-old bayberry trees planted on 43°slope land was the earliest, which was 1-3 days earlier than that of bayberry planted on 28° and 15° slope land, respectively. Compared with that planted on 28° and 15° slopes, the yield of 7-year-old bayberry planted on 43° slope increased by 50.00% and 87.72%, respectively. Compared with the bayberry planted on 15° slope, the single fruit weight of the 7-year-old bayberry planted on 43° slope increased by 19.13%. The soluble solids content of bayberry planted on 28° and 43° slopes were better, which were 1.27 and 1.45 percentage points higher than that on 15°slope, respectively. The total sugar content of bayberry planted on 28° and 43° slopes was 10.60% and 13.39% higher than that of bayberry planted on 15° slope, respectively, and the total acid content was 8.76% and 11.68% lower than that of bayberry planted on 15° slope, respectively. The sugar-acid ratios of bayberry planted on 28° and 43° slope were 12.63 and 13.38, respectively. The planting of slope land is conducive to the early maturity of Dongkui bayberry, the increase of plant yield, the increase of single fruit weight, and the increase of sugar-acid ratio, among which the Dongkui bayberry planted on 43° slope land has early fruit, large fruit shape and excellent quality, which can be popularized and applied in the planting of bayberry in mountainous areas. Figures and Tables | References | Related Articles | Metrics

Select

Effects of different green manures on soil fertility and plant growth in citrus orchards WEN Mingxia, HUANG Bei, WANG Peng, WU Shaohui 2025, 66 (3):  662-666.  DOI: 10.16178/j.issn.0528-9017.20240107 Abstract ( 30 )   HTML ( 3 )   PDF (1207KB) ( 105 )   To explore the effects of planting different green manure on soil fertility and plant growth in young citrus orchards, and to provide a theoretical basis for rational planting of green manure, rattail fescue, ryegrass and hairy vetch were planted in young citrus orchards for three consecutive years, and the biological yield and nutrient content of green manure were measured at the full flowering stage, and soil samples were collected in citrus orchards for 100 days to analyze soil physical and chemical properties. The results showed that there were significant differences in the yield of rattail fescue, ryegrass and hairy vetch treatments, and the yield of ryegrass and hairy vetch was 102.0% and 194.2% higher than that of rattail fescue, respectively. The total N and P contents of the three green manures were hairy vetch>rattail fescue>ryegrass. The total K content of rattail fescue and hairy vetch was basically the same, and was significantly higher than that of ryegrass. The height of ryegrass in full flowering was significantly higher than that of rattail fescue and hairy vetch. There was no significant difference in soil phosphatase and catalase activities in different green manure growth areas, and the nitrate reductase activities were rattail fescue>ryegrass>hairy vetch, while urease activities were ryegrass>rattail fescue>hairy vetch, and the differences among the three were significant. There were significant differences in soil available nitrogen, phosphorus and potassium contents among different green manure growth areas, and the highest treatment was hairy vetch. The number of citrus new shoot in different green manure planting areas was significantly higher than that of the control, and the number of new shoot was the highest in the treatment with hairy vetch. The length of the new shoot was shown as ryegrass>hairy vetch>rattail fescue>CK. The shoot thickness and leaf number of hairy vetch and ryegrass treatments were significantly higher than those of the control. It can be seen that planting different green manure can improve the microecological environment in the root zone, increase soil enzyme activity and soil fertility, and promote plant growth. When it comes to fertilizing the soil, the hairy vetch have the best effect. As orchard intercropping green manure, the height of rattail fescue and hairy vetch was more suitable in the full flowering period, and when intercropping ryegrass, it was recommended to mow and return to the field when the height is 20-40 cm. Figures and Tables | References | Related Articles | Metrics

Select

Comprehensive evaluation of 26 varieties of Hydrangea macrophylla in coastal area of Tianjin NIU Zhe, ZHAI Tongtong, YU Lu, LIU Wei, HAO Liyuan, ZHANG Leixiao, YE Xiaorui 2025, 66 (3):  667-673.  DOI: 10.16178/j.issn.0528-9017.20231104 Abstract ( 25 )   HTML ( 3 )   PDF (2803KB) ( 107 )   To screen out the suitability for landscape greening in Tianjin coastal area, 26 varieties of test materials with good growth conditions and strong stress resistance were selected from 36 varieties of Hydrangea macrophylla. 9 indexes of branches, leaves, and flowers were studied with the application of the grey correlation analysis. The results showed that significant differences in weighted correlation value among 26 varieties of Hydrangea macrophylla were detected, which ranged from 0.479-0.834. Six varieties including Taube, Utaawase, Forever Summer, Temari and Ivetta had higher relative correlation values (>0.7), which means strong ecological adaptability and outstanding ornamental value in this area. These excellent ornamental varieties could be used as high-quality provenances for further research and promotion on the landscaping of Tianjin coastal area. Figures and Tables | References | Related Articles | Metrics

Select

Research progress on development and utilization of lotus seed by-products WANG Yeyang, LI Weirong, HUANG Sha, YANG Dongting, YANG Xuan 2025, 66 (3):  674-680.  DOI: 10.16178/j.issn.0528-9017.20231173 Abstract ( 26 )   HTML ( 2 )   PDF (1241KB) ( 102 )   Lotus seedpod, lotus seed epicarp, lotus seed skin, lotus plumule are by-products of lotus seed production. These by-products are usually regarded as wastes and directly discarded or buried in the field, resulting in a waste of resources. However, the by-products of lotus seeds contain ample bioactive ingredients which have anti-inflammatory, anti-bacteria, hypoglycemic, anti-obesity, anti-cancer activities. After processing, these by-products have application prospects in medicine, food, textile, electrochemistry and other fields. In this review, the main bioactive ingredients, biological effects, extraction of active ingredients and their related development and utilization of lotus seed by-products were reviewed, in order to provide reference for extending the lotus seed industry chain and further development and utilization of lotus seed by-products. References | Related Articles | Metrics

Select

Study on the disease and pest risk of white cucumber germplasm resources in Hangzhou and its control technique WU Yanjun, HONG Wenying, ZHU Xuyan, HUA Yonggang, WANG Qun, NI Jinzhuang, HU Qinmian, CHEN Rui 2025, 66 (3):  681-686.  DOI: 10.16178/j.issn.0528-9017.20240560 Abstract ( 25 )   HTML ( 2 )   PDF (1875KB) ( 99 )   In this study, we investigated the occurrence of pests and diseases of 3 local varieties of white cucumber germplasm resources collected from Hangzhou area, analyzed their susceptibility to diseases and pests, explored the disease and pest risk and developed the control technique for them. The results showed that under the condition of natural occurrence, different germplasms of white cucumbers have significant differences in the susceptibility to diseases and pests. The most susceptible germplasm to seedling disease was 2022CA1-1. Downy mildew was the most susceptible disease in the field period, and the most susceptible germplasm was 2022CA1-2 whose peak disease index of spring and autumn cucumber were 77.89 and 80.04, respectively. The susceptibility of 2022CA1-1 and 2022CA1-2 to the bacterial angular leaf spot were moderate which showed higher than 2022LH1-1. The most susceptible germplasm to powdery mildew was 2022CA1-2 which led to heavy occurrence in the middle and late growth stage. Aphis gossypii, Bemisia tabaci, Thrips palmi were the most susceptible pests, and Diaphania indica was moderate. Liriomyza sativae and Tetranychus cinnbarinus could be often investigated but less amount of occurrence. The incidence extent of all major pests had no obvious difference between different germplasms of white cucumber. On the basis of susceptibility analysis results to pests and diseases in various germplasm, the green techniques against different stages which included preplanting, seedling and field period were formulated. The findings of this study could be useful for breeding and utilization of resistance germplasm in white cucumber. Figures and Tables | References | Related Articles | Metrics

Select

Study on the construction of knowledge graph for diseases and pests of citrus ZHU Yihang, ZHANG Xiaomin, ZHOU Tianqi, RAO Xiuqin 2025, 66 (3):  687-691.  DOI: 10.16178/j.issn.0528-9017.20231218 Abstract ( 21 )   HTML ( 2 )   PDF (4558KB) ( 101 )   In traditional cultivation of citrus, farmers lack abundant professional agricultural knowledge and technology. Information technology can digitize the knowledge of pest and disease control of citrus. However, current knowledge management technologies have some common deficiencies in terms of efficiency, scalability, and applicability. Knowledge graph provides a new approach to knowledge management, which is a more flexible method. Considering the insufficient detailed characterization of entities and relationships related to pest and disease control of citrus in existing agricultural knowledge graphs, this study defines entity types and relationship categories for pest and disease knowledge of citrus. A total of 10 entity categories and 10 entity relationships were identified, and a large language model was used for extraction and deduplication, addressing 16 common deficiencies. Finally, the knowledge graph was stored and visualized using the Neo4j database. The discussion focused on how knowledge graph for pest and disease of citrus can provide underlying technical support for downstream tasks such as precise pest and disease information inquiry and intelligent-assisted diagnosis. Figures and Tables | References | Related Articles | Metrics

Select

Screening of control agent for Collembola in Fritillaria thunbergii WU Jun, XIE Yunye, FANG Li, WANG Hanrong 2025, 66 (3):  692-696.  DOI: 10.16178/j.issn.0528-9017.20231190 Abstract ( 22 )   HTML ( 1 )   PDF (1165KB) ( 117 )   Fritillaria thunbergii is one of the “Eight Flavors of Zhejiang” and a characteristic economic crop in Zhejiang Province. For the new pathogen Collembola, thirty-four chemical control agents were selected in this study, and used for determining the effectiveness of these agents. The results showed that rotenone, zjd19-72 and avermectin had the best effectiveness on Collembola. This study will provide a basis for further field experiments. Figures and Tables | References | Related Articles | Metrics

Select

Efficacy of low sugar to vinegar ratio attractants against fruit flies on Chinese bayberry LIANG Senmiao, YU Zheping, WANG Jingbo, SUN Li, QI Xingjiang, ZHANG Shuwen 2025, 66 (3):  697-701.  DOI: 10.16178/j.issn.0528-9017.20231230 Abstract ( 20 )   HTML ( 3 )   PDF (1178KB) ( 99 )   Fruit flies are one of the hazards that affect the production efficiency of Chinese bayberry, and attractants are effective measures to lure and kill fruit flies. This study analyzed the correlation between the quality indicators of Chinese bayberry fruit and the number of fruit fly larvae. It was found that the total acid content, citric acid content, malic acid content, and oxalic acid content of the Chinese bayberry fruit were significantly positively correlated with the number of fruit fly larvae. Further adjustments were made to the ratio of sugar to vinegar, and high sugar to vinegar ratio (2∶1) and low sugar to vinegar ratio (1∶2) fruit fly attractants containing microbial source attractants were formulated. Different cultivation methods, such as trellis cultivation and early facility cultivation, were set up to analyze the control effect of different cultivation methods on fruit flies with open field cultivation as a control. The results showed that the early facility cultivation had the highest control effect (77.57%)on fruit files, followed by trellis cultivation (71.96%), and the low sugar to vinegar ratio attractant had a significantly higher control effect (65.58%) than the high sugar to vinegar ratio attractant (32.87%); The usage parameters of low sugar to vinegar ratio attractants had been clarified. About 20 days before harvesting, they are hung on the outer branches of the tree about 1.5 meters above the ground, with one bottle hung with one tree in between (about 10 meters apart). The prevention effect can reach 64.01%. This method is a fruit fly trapping technique with obvious prevention effect, low cost and long-lasting effectiveness. Figures and Tables | References | Related Articles | Metrics

Select

Identification of the pear mold spot pathogen in Haining TANG Jiejing, ZHENG Fang, RUAN Zanyu, LI Hongye 2025, 66 (3):  702-705.  DOI: 10.16178/j.issn.0528-9017.20240529 Abstract ( 21 )   HTML ( 2 )   PDF (4762KB) ( 95 )   Pear is the third important fruit in China and constitutes an important component of the fruit industry in Zhejiang Province. In recent years, a spot disease of unknown etiology has been prevalent in pear orchards and caused economic losses in Haining, Jiaxing, Zhejiang. In this study, we collected diseased leaves and fruits from five pear trees in the orchards of Qianjiang Village, Huangwan, Haining, Jiaxing, using a diagonal sampling method. The morphology of the fruiting bodies was observed under a stereomicroscope, and the isolation and purification of supspected pathogen were conducted on 50 diseased leaves and 50 diseased pears. The results of morphology obsenation of fruiting bodies showed that the pathogen was a species of Cercosporioid. Furthermore, molecular identification of 13 isolates was performed with rDNA-ITS sequencing, and the results showed a 99.8% sequence identity with the ITS sequence of Pseudocercospora mali strain MUCC 886. Therefore, we proposed that the pathogen of pear mold spot in Haining was Pseudocercospora mali. In this study, the symptoms of pear mold spot on fruits was described at the first time, and the results provided a reference for the control of pear mold spot. Figures and Tables | References | Related Articles | Metrics

Select

Fluid ice preservation technology and its effects on storage quality of fresh food GUO Taike, ZHAO Dandan, WENG Yayi, WANG Keying, HE Yuanhai, YAO Jigang, CHEN Wenxuan 2025, 66 (3):  706-711.  DOI: 10.16178/j.issn.0528-9017.20240523 Abstract ( 29 )   HTML ( 3 )   PDF (1219KB) ( 98 )   Pre-cooling treatment is necessary for cold chain distribution of fresh food. Efficient pre-cooling treatment can effectively slow down food spoilage. As a new type of green cold medium, fluid ice has the advantages of high cooling rate, high energy storage density, convenient transportation, and can maintain the properties of fresh food without causing mechanical damage. In this study, the basic properties and preparation methods of fluid ice were summarized, and the effects of fluid ice on storage quality of fresh food were discussed from four aspects: microorganism, protein, K value and shelf life. The application of fluid ice coupled with ozone, acidic electrolyzed water, natural oxidants, and other technologies in food preservation was reviewed, and the prospects of fluid ice pretreatment technology were also prospected. Figures and Tables | References | Related Articles | Metrics

Select

Enzymatic hydrolysis process of citrus peel and its effect on the production characteristics of reducing sugars and flavonoids as active products TAO Anan, WU Yuejuan, GU Pin, LUO Zhe, YUAN Feng, QIU Hao, LI Guolei 2025, 66 (3):  712-718.  DOI: 10.16178/j.issn.0528-9017.20231177 Abstract ( 19 )   HTML ( 3 )   PDF (1710KB) ( 98 )   In order to promote the high-value resource utilization of discarded citrus peels, including rotten citrus fruits, through biological enzymatic conversion, the effects of a composite enzyme composed of cellulase and pectinase on the enzymatic hydrolysis of citrus peels and the production characteristics of their active products, reducing sugars and flavonoids, were investigated based on single factor changes such as cellulase and pectinase addition, composite enzyme ratio, enzymatic hydrolysis temperature, pH value, substrate concentration, and enzymatic hydrolysis time. The results showed that the optimized process parameters for the enzymatic hydrolysis of citrus peel by composite enzyme were the substrate concentration (citrus peel∶water) of 1∶2, the total addition of 0.10% cellulase and pectinase (enzyme ratio 1∶1), the pH value of 4.5, the temperature of 55 ℃, and the time of 24 h. Under these conditions, the degradation rate of citrus peel, the content of reducing sugars in the solution, and the extraction amount of flavonoids reached the maximum, which were 99.80%, 10.14%, and 20.37 mg·g-1, respectively. Figures and Tables | References | Related Articles | Metrics

Select

Characterization of quinolone residues in leafy vegetables from growing areas around chicken farms LIU Aichun, ZHA Yan, BAI Junhui, HE Xin, LIU Chao 2025, 66 (3):  719-723.  DOI: 10.16178/j.issn.0528-9017.20231175 Abstract ( 21 )   HTML ( 1 )   PDF (1370KB) ( 97 )   In this study, the content of quinolones in leafy vegetables from four chicken farms around Hangzhou City was analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results clarified that the detection rates of enrofloxacin (ENR), ciprofloxacin (CIP), norfloxacin (NOR) and lomefloxacin (LOM) in different varieties of leafy vegetables ranged from 41% to 100%, and the total content of quinolones(Σ QNs) ranged from 0.89-95.74 μg·kg-1, of which 10.00-50.00 μg·kg-1accounted for 50.06%, and the proportions below 10.00 μg·kg-1and above 50.00 μg·kg-1 were 41.47% and 8.47% respectively, with an average of 11.83 μg·kg-1.The average of quinolone residues in different varieties was:Chinese cabbage>leafy lettuce>lettuce>spinach. The detection rate of NOR in leafy vegetables was as high as 100%, and its maximum and average values were 70.31 and 9.44 μg·kg-1, respectively, which were much higher than the other three drugs. Although the content of QNs in leafy vegetables is not high, the long-term low-dose intake of antibiotics is easy to induce bacterial resistance, so the environmental impact of the irrational use of antimicrobials should not be ignored. Figures and Tables | References | Related Articles | Metrics

Select

Acute toxicity test of three fishery drugs to Konosirus punctatus LI An, MA Jianzhong, SHAN Lezhou, REN Peng, SHAO Xinbin 2025, 66 (3):  724-29.  DOI: 10.16178/j.issn.0528-9017.20240602 Abstract ( 20 )   HTML ( 2 )   PDF (1220KB) ( 100 )   In order to study the acute toxicity and explore the safety concentration of methylene blue, copper sulfate and ferrous sulfate mixture(5∶2) and povidone-iodine to juvenile Konosirus punctatus, the acute toxicity test of three fishery durgs to juvenile Konosirus punctatus with body length of (2.68±0.24)cm and body weight of (1.97±0.23)g were investigated at water temperature of (25±0.6)℃, pH value of 7.43±0.33 and dissolved oxygen content>6.56 mg·L-1. The results showed that the half lethal concentrations(LC50) of methylene blue, copper sulfate and ferrous sulfate mixture(5∶2) and povidone-iodine to juvenile Konosirus punctatus under 24 hours' treatment were 36.282 mg·L-1, 8.984 mg·L-1 and 7.004 mg·L-1, respectively. The LC50 under 48 hours' treatment were 29.577 mg·L-1, 7.545 mg·L-1 and 6.551 mg·L-1, respectively. The LC50 under 72 hours' treatment were 28.199 mg·L-1, 7.400 mg·L-1 and 6.031 mg·L-1, respectively. The LC50 under 96 hours' treatment were 24.613 mg·L-1, 7.063 mg·L-1 and 5.900 mg·L-1, respectively. The safety concentration of methylene blue, copper sulfate and ferrous sulfate mixture(5∶2) and povidone-iodine to juvenile Konosirus punctatus were 2.461 mg·L-1, 0.706 mg·L-1 and 0.590 mg·L-1, respectively. According to the evaluation criteria for toxicity of toxic substances to fish, methylene blue was low toxic drug, copper sulfate and ferrous sulfate mixture(5∶2) and povidone-iodine were medium toxic drugs. Figures and Tables | References | Related Articles | Metrics

Select

Study on honey identification based on fluorescence quantitative PCR method WANG Xiaoyu, WU Pan, WANG Chengli, YUE Wanfu 2025, 66 (3):  730-736.  DOI: 10.16178/j.issn.0528-9017.20240068 Abstract ( 17 )   HTML ( 1 )   PDF (1341KB) ( 99 )   This study explored the applicability of real-time fluorescence quantitative PCR in the identification of honey. The pollen DNA and bee DNA in honey samples were extracted by CTAB method. The DNA in honey was detected by real-time fluorescence quantitative PCR using five plants and bee DNA primer probes to determine the pollen composition, production time and production location of different honey samples. The feasibility of the method was verified by 17 kinds of honey from unknown sources, and the results showed that the method was feasible. This study plays a role in promoting and guiding the use of molecular genetics for honey identification. Figures and Tables | References | Related Articles | Metrics

Select

Research and application progress of efficient production technology of egg pigeon HOU Fengxiang, JIN Junjie, SONG Bin, ZHANG Yawen, LI Ziwei, ZHAO Yanwen, GAN Peiling, ZHAO Yan 2025, 66 (3):  737-741.  DOI: 10.16178/j.issn.0528-9017.20231085 Abstract ( 30 )   HTML ( 3 )   PDF (1170KB) ( 104 )   With the growth of the national economy and the gradual improvement of residents' living standards and consumption ability, the demand for high-quality livestock and poultry products is increasing. Pigeon eggs are highly favored by consumers for their unique nutritional content. However, due to the unique biological characteristics of pigeons, their egg production is not high, and there are currently no specialized pigeon breeds for egg production, mostly of the meat and egg dual-use type. In order to promote efficient production of egg pigeons and increase breeding benefits, this article intends to summarize the selection of egg pigeon breeds, male and female identification, double female pairing techniques, and prevention and control of Trichomonas gallinae, in order to provide reference for pigeon breeders. References | Related Articles | Metrics

Select

Research progress of immunoassay methods in the detection of ofloxacin residues YANG Xingdong, QU Yang, HU Xiaofei, LI Lili 2025, 66 (3):  742-749.  DOI: 10.16178/j.issn.0528-9017.20240489 Abstract ( 22 )   HTML ( 1 )   PDF (2700KB) ( 100 )   Ofloxacin (OFLX), a major kind of fluoroquinolones(FQs), is commonly used in human and animal healthcare due to its antimicrobial properties and low cost. However, the risk to human health posed by its residues has attracted considerable attention. In view of the residues of OFLX in the food and environment, it is imperative to establish its immediate detection. Compared with traditional chromatographic and spectroscopic detection techniques, immunoassay has outstanding advantages in terms of specificity, detection efficiency, field applicability and simplicity of pre-treatment. This paper reviewed the preparation of OFLX antibodies and the current application of immunoassay techniques in OFLX residue detection, compared the advantages and disadvantages of enzyme-linked immunoassay, immunochromatographic method, fluorescence immunoassay, immunosensor, bio-barcode immunoassay, and nucleic acid aptamer, etc., and looked forward to the development trend of the immunoassay methods of OFLX with the aim of providing a reference for the establishment and application of rapid antibiotic detection methods. Figures and Tables | References | Related Articles | Metrics

Select

Current situation and application of acidified farmland management in southern China—a case study of Zhejiang Province WANG Jie, JI Weiying, GU Jianqiang, ZHANG Yuanyuan, LIU Xiaoxia 2025, 66 (3):  750-759.  DOI: 10.16178/j.issn.0528-9017.20240551 Abstract ( 33 )   HTML ( 2 )   PDF (1373KB) ( 112 )   Soil acidification not only causes aluminum toxicity to crops, deterioration of soil structure, decline of nutrient availability and biodiversity, resulting in a significant reduction in crop yield or even no harvest, but also activates heavy metals, causing quality and safety problems of agricultural products, which seriously threatens food security, ecological environment security and sustainable development of agriculture, especially in southern China. It is more common and urgent to carry out large-scale effective governance. Taking Zhejiang Province as an example, which is one of the typical agricultural modernization provinces in the south of China, this paper discussed the current situation of acidified cultivated land and its possible influencing factors based on the years of acidified cultivated land management in Zhejiang Province. The strategy and technical path of acidified cultivated land management were put forward from the technical level and the working level. The application examples and effects of acidified cultivated land management were discussed from paddy field, dry land, orchard land, tea garden land and other types. The existing problems of acidified cultivated land management at the present stage were analyzed. The corresponding suggestions and prospects were put forward, such as expanding the coverage of acidified cultivated land management, integrating the efficient technical model of the base, and increasing the degree of attention. It provides a reference for the management of acidified cultivated land in China. Figures and Tables | References | Related Articles | Metrics

Select

Profile analysis of soil bacterial community distribution and nitrogen metabolism in citrus orchard under combined application of animal and poultry organic fertilizer and chemical fertilizer TONG Wenbin, ZHENG Zehua, YANG Haijun, LI Ronghui, YE Zhengqian 2025, 66 (3):  760-768.  DOI: 10.16178/j.issn.0528-9017.20240838 Abstract ( 22 )   HTML ( 2 )   PDF (7275KB) ( 109 )   In order to investigate the changes in the distribution and function of bacterial communities in the soil profiles of citrus under the combined application of animal and poultry organic fertilizers and chemical fertilizers, a typical citrus orchard in Quzhou City was selected as the research sample. The soil physicochemical properties of two varieties of citrus, Penggan and Xingjin, were analyzed under different profiles 0-20 cm(≤20 cm)and >20-40 cm. The results showed that soil pH value in 0-20 cm soil layer was significantly lower than that in >20-40 cm soil layer(P<0.05), and the contents of total nitrogen(TN), total phosphorus(TP), microbial biomass carbon(MBC), microbial biomass nitrogen(MBN) and microbial biomass phosphorus(MBP) in >20-40 cm soil layer were significantly higher than those in >20-40 cm soil layer(P<0.05). The results of bacterial community analysis showed that the diversity and abundance of bacteria in 0-20 cm soil layer were higher than those in >20-40 cm soil layer. The dominant bacterial phyla phyla in the soil layer were Pseudomonadota, Acidobacteriota and Actinomycetota. In addition, the activities of enzymes related to nitrogen metabolism were higher in shallow soil(0-20 cm). Correlation analysis showed that Acidobacterium, Actinophytocola and other dominant bacterial phyla were significantly positively correlated with TN, TP, MBC, MBN and MBP contents(P<0.05), and significantly negatively correlated with pH value contents(P<0.05). In general, compared with conventional fertilization, the combination of animal and poultry organic fertilizer and chemical fertilizer optimized the physical and chemical properties of 0-20 cm soil layer, improved the bacterial diversity and functional activity of 0-20 cm soil layer in citrus orchard, but had little effect on >20-40 cm soil layer. Figures and Tables | References | Related Articles | Metrics

Select

Research progress on molecular mechanism and molecular genetic breeding of plant salt tolerance WANG Weiwei, SHEN Feng, WU Yongcheng, MEI Yi, ZHENG Jiaqiu, ZU Yanxia, LIU Zhe, ZHANG Lina, FENG Ruchao 2025, 66 (3):  769-775.  DOI: 10.16178/j.issn.0528-9017.20231148 Abstract ( 26 )   HTML ( 3 )   PDF (1254KB) ( 103 )   Soil salinization is a major abiotic stress factor affecting plant growth and development. In this paper, salt stess to plant growth, the molecular mechanism of plant response to salt stress and the research progress of molecular genetic breeding for plant salt tolerance were introduced, and the research prospect was prospected, which could provide theoretical reference for the cultivation and improvement of plant salt tolerance. References | Related Articles | Metrics

Select

Analysis on the path of high-efficiency eco-agriculture in amplifying the common prosperity effect in counties LUO Lijun 2025, 66 (3):  776-783.  DOI: 10.16178/j.issn.0528-9017.20240769 Abstract ( 20 )   HTML ( 1 )   PDF (1202KB) ( 101 )   The unique advantages of green ecology, traditional agricultural products, and potential value enhancement are the core problems encountered in the process of rural industrial revitalization. The article takes Hu pomelo industry as an example, focusing on the leading industries with county-level characteristics. Through the three major channels of economy, ecology, and culture, relying on the innovative effects, balance effects, spillover effects, transformation effects, and sharing effects of efficient ecological agriculture, it consolidates regional advantages, enhances product value, and amplifies the county's common prosperity effect. References | Related Articles | Metrics

Select

Industry development status and countermeasure analysis of Trapa acornis Nakano in Jiaxing YUAN Ye, XU Yicheng, SHEN Meng, QUAN Xinhua, WANG Ruisen, YAO Xiangtan, ZHANG Min 2025, 66 (3):  784-788.  DOI: 10.16178/j.issn.0528-9017.20231111 Abstract ( 28 )   HTML ( 2 )   PDF (1796KB) ( 101 )   Trapa L. is one of the important aquatic economic crop in Jiaxing, Trapa acornis Nakano is a famous and traditional cultivars with local characteristics in Jiaxing because of its high nutritional value, delicious taste and deep cultural heritage with a long cultivation history. Through investigation and analysis of the industry development status of Trapa acornis Nakano in Jiaxing, this paper pointed out some problems such as the reduction of production scale and serious variety degradation of Trapa acornis Nakano, put forward some countermeasures such as the rejuvenation and creation of high-quality varieties of Trapa acornis Nakano, innovation of supporting and efficient cultivation mode, standardization of production technical standards of Trapa acornis Nakano, and development of multi-functional by-products of Trapa acornis Nakano. In order to promote healthy and sustainable development of Trapa acornis Nakano industry in Jiaxing, the countermeasures are necessary to enhance the comprehensive planting benefit, improve the purity and quality of varieties and increase the added value of germplasm resources of Trapa acornis Nakano. Figures and Tables | References | Related Articles | Metrics

Select

Strategies for enhancing the system of science and technology commissioner in the context of comprehensive rural revitalization JING Yuting 2025, 66 (3):  789-793.  DOI: 10.16178/j.issn.0528-9017.20240974 Abstract ( 25 )   HTML ( 3 )   PDF (1206KB) ( 103 )   The system of science and technology commissioners has been implemented for more than 20 years, making significant contributions to the development of “agriculture, rural areas, and farmers". In the new era, the new goals of comprehensive rural revitalization and modernization development pose new requirements and challenges to the system of science and technology commissioners. There are some problems in the traditional system of science and technology commissioners in terms of service content, management mode, resource integration and service guarantee. Based on the investigation of the operation practice of the system of science and technology commissioners in five provinces(autonomous regions), namely Zhejiang, Fujian, Guangdong, Anhui and Ningxia, this paper has put forward optimization and enhancement strategies for the innovation of the system of science and technology commissioners in the new situation. Figures and Tables | References | Related Articles | Metrics

Select

Study on the construction of agricultural science and technology talent teams in mountainous areas under the background of rural revitalization—a case study of Chun'an County, Zhejiang Province FANG Weihong, LIU Lingmin, XU Zhiyuan, SHENG Menghan, KE Fuyan 2025, 66 (3):  794-800.  DOI: 10.16178/j.issn.0528-9017.20250057 Abstract ( 34 )   HTML ( 1274019843 )   PDF (1191KB) ( 113 )   With the rapid development of science and technology, the key role of agricultural science and technology personnel in rural revitalization has become increasingly prominent, especially in the mountainous area, which is the key and difficult area of rural revitalization. Taking Chun'an County, Zhejiang Province as an example, this study systematically discussed the practice path and optimization strategy of agricultural science and technology talent team construction under the background of rural revitalization through conference and individual interview. It is found that Chun'an County has made some achievements in the construction of agricultural science and technology talents, but there are still problems such as imperfect management mechanism, shortage of high-quality professional talents, lagging innovation platform construction and weak rural front-line personnel. In response to these problems, the paper puts forward countermeasures and suggestions such as optimizing the talent management system, expanding the agricultural science and technology personnel, strengthening the construction of high-level science and technology platform carrier and improving the quality of rural workers, aiming at providing theoretical basis and practical reference for the construction of agricultural science and technology personnel in mountainous areas. Figures and Tables | References | Related Articles | Metrics