Preliminary screening of leaf regeneration systems of five cultivated strawberry varieties

doi:10.16178/j.issn.0528-9017.20231121

Abstract

Abstract:

In order to alleviate the problem that the number of stem tips of some varieties could not meet the production demand of virus-free strawberry seedlings, and screen different strawberry varieties of the most suitable leaf age for leaf induction in vitro, Taoxun, Poinsettia, Suizhu, Qianberry 1 and Yuexiu were used as experimental materials. The effects of in vitro induction medium (MS+0.5 mg·L^-1 6-BA+1.5 mg·L^-1TDZ+0.2 mg·L^-1NAA) on different strawberry varieties and leaf ages were studied. At the same time, the effects of different carving methods on callus induction were studied by using Suizhu leaves as explants. The results showed that the induction rate of adventitious buds in Poinsettia and Taoxun was higher under MS+0.5 mg·L^-1 6-BA+1.5 mg·L^-1 TDZ+0.2 mg·L^-1 NAA. Suizhu is suitable for the treatment of mixed carving methods on the edge and back of leaves, which is beneficial to improve the callus induction rate. The above results showed that some strawberry varieties could be induced in vitro by the same medium which could improve the production efficiency of virus-free seedlings. The callus quantities of some strawberry varieties was affected by different carving methods. The callus induction rate of leaves could be improved by selecting suitable carving methods.

Key words: strawberry, leaf, callus, adventitious bud

CLC Number:

S668.4

LI Wenqing, YANG Zhengwei, MENG Geng, WANG Yiyi, HOU Juan, WANG Zhihao, SUN Kaile. Preliminary screening of leaf regeneration systems of five cultivated strawberry varieties[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(1): 120-126.

Figures/Tables 7

References 34

[1]	严聪文, 苏代发, 代庆忠, 等. 草莓病害的生物防治研究进展[J]. 生物技术通报, 2022, 38(12): 73-87.
[2]	徐耀. 不同药剂对草莓蚜虫防治效果的研究[J]. 农业科技通讯, 2022(4): 177-179.
[3]	王建辉, 徐瑞, 龚晓源, 等. 草莓组培苗的病毒检测与其果实品质分析[J]. 北方园艺, 2023(2): 1-9.
[4]	VALENTOVA L, REJLOVA M, FRANOVA J, et al. Symptomless infection by strawberry virus 1 (StrV-1) leads to losses in strawberry yields[J]. Plant Pathology, 2022, 71(5): 1220-1228.
[5]	TORRICO A K, CELLI M G, CAFRUNE E E, et al. Genetic variability and recombination analysis of the coat protein gene of Strawberry mild yellow edge virus[J]. Australasian Plant Pathology, 2016, 45(4): 401-409.
[6]	MARTIN R R, TZANETAKIS I E. High risk strawberry viruses by region in the United States and Canada: implications for certification, nurseries, and fruit production[J]. Plant Disease, 2013, 97(10): 1358-1362.
[7]	NAING A H, KIM S H, CHUNG M Y, et al. In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars[J]. Plant Methods, 2019, 15: 36.
[8]	DIAZ-LARA A, STEVENS K A, KLAASSEN V, et al. Sequencing a strawberry germplasm collection reveals new viral genetic diversity and the basis for new RT-qPCR assays[J]. Viruses, 2021, 13(8): 1442.
[9]	张立磊, 李桂荣, 张浩, 等. 不同草莓品种无菌苗增殖及不定芽诱导的研究[J]. 西北林学院学报, 2018, 33(2): 134-138, 172.
[10]	施苏丽, 薛姣, 周佐葡, 等. 不同品种彩色马蹄莲灭菌条件优化与不定芽诱导[J]. 分子植物育种, 2019, 17(13): 4398-4403.
[11]	韦献雅, 谢尚春, 欧阳丽莹, 等. 茎尖脱毒技术的优化研究: 以草莓、丹参、魔芋为例[J]. 耕作与栽培, 2022, 42(4): 131-135.
[12]	WANG M R, BI W L, BETTONI J C, et al. Shoot tip cryotherapy for plant pathogen eradication[J]. Plant Pathology, 2022, 71(6): 1241-1254.
[13]	郝征, 靳茜, 何富强, 等. 草莓种苗脱毒及繁育技术研究进展[J]. 分子植物育种, 2023, 21(12): 4006-4013.
[14]	刘畅, 王晓, 李宪松, 等. 我国草莓生产态势及国内外比较分析[J]. 中国果树, 2023(7): 136-140.
[15]	姜福星, 黄远祥, 周鹏, 等. 植物叶片再生的探究[J]. 分子植物育种, 2018, 16(20): 6832-6839.
[16]	王淑珍, 马华升, 童建新, 等. 草莓叶片离体再生技术的研究[J]. 农业科技通讯, 2010(7): 83-85.
[17]	NERI J C, MELÉNDEZ-MORI J B, TEJADA-ALVARADO J J, et al. An optimized protocol for micropropagation and acclimatization of strawberry (Fragaria×ananassa duch.) variety ‘Aroma’[J]. Agronomy, 2022, 12(4): 968.
[18]	PASSEY A, BARRETT K, JAMES D. Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria×ananassa Duch.) using a range of explant types[J]. Plant Cell Reports, 2003, 21(5): 397-401.
[19]	JIANG S, JI Y, WANG M Q, et al. Establishing a high-efficiency in vitro regeneration system for Agrobacterium-mediated transformation in Fragaria nilgerrensis[J]. Fruit Research, 2023, 3(1).
[20]	朱海生, 潘东明, 林义章, 等. 草莓高频离体再生体系的研究[J]. 西北植物学报, 2007, 27(5): 919-925.
[21]	李玲, 黄榕, 艾薇, 等. 香椿离体再生技术研究进展[J]. 浙江农业科学, 2023, 64(2): 366-370.
[22]	CHUNG H H, OUYANG H Y. Use of thidiazuron for high-frequency callus induction and organogenesis of wild strawberry (Fragaria vesca)[J]. Plants, 2020, 10(1): 67.
[23]	CAPPELLETTI R, SABBADINI S, MEZZETTI B. The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars[J]. Scientia Horticulturae, 2016, 207: 117-124.
[24]	杜梦卿, 连朋, 王丽娟. 不同浓度TDZ和2,4-D组合对草莓花药组织培养的影响[J]. 东北农业科学, 2021, 46(2): 73-75, 131.
[25]	黄菲颖. ‘紫精灵’紫薇组织培养及内源激素含量变化研究[D]. 长沙: 中南林业科技大学, 2022.
[26]	周厚成, 罗静, 赵霞, 等. 不同培养条件对幸香草莓离体叶片再生的影响[J]. 果树学报, 2007, 24(1): 105-108.
[27]	张庆瑞, 杨秋生, 李永华. 不同植物生长调节物质对芍药离体培养的影响[J]. 河南农业大学学报, 2007, 41(1): 25-28.
[28]	李晓青, 王晓云, 张晓申, 等. 草莓启动培养技术研究[J]. 农业科技通讯, 2016(2): 132-133.
[29]	蒲忠贵, 张庆全. 草莓茎尖组织培养技术研究[J]. 甘肃农业科技, 2022, 53(9): 86-89.
[30]	沈莉, 曹玉梅. 7个品种草莓茎尖组培脱毒苗技术研究[J]. 青海农林科技, 2022(2): 96-100.
[31]	李慧. 红颜草莓的离体培养与快速繁殖[J]. 江苏农业科学, 2015, 43(11): 66-68.
[32]	张雪. 草莓叶片离体再生不定芽研究[J]. 江西农业, 2019(6): 43.
[33]	杨仕品, 钟霈霖, 乔荣. 不同培养因子对草莓离体叶片再生的影响[J]. 贵州农业科学, 2013, 41(9): 30-32.
[34]	凤婷, 赵密珍, 王钰, 等. 草莓品种宁玉再生体系的建立[J]. 江苏农业学报, 2013, 29(3): 688-690.

编号	品种名称	品种来源	果皮颜色
1	桃熏	日本茨城县	粉白色
2	圣诞红	西班牙	红色
3	隋珠	日本三重县农业研究所	深红色、淡红色
4	黔莓1号	贵州省园艺研究所	红色
5	越秀	浙江农科院园艺研究所	红色

编号	品种名称	品种来源	果皮颜色
1	桃熏	日本茨城县	粉白色
2	圣诞红	西班牙	红色
3	隋珠	日本三重县农业研究所	深红色、淡红色
4	黔莓1号	贵州省园艺研究所	红色
5	越秀	浙江农科院园艺研究所	红色

品种	叶龄/d	出愈率/%	不定芽诱导率/%	不定芽增殖系数
桃熏	15~25	100.00±0 a	15.60±1.24 a	2.00±0.43 ab
	25~35	100.00±0 a	3.30±0.58 c	1.00±0.79 bc
	35~45	100.00±0 a	6.70±1.15 b	1.50±0.41 ab
圣诞红	15~25	100.00±0 a	14.60±1.15 a	1.50±0.29 ab
	25~35	100.00±0 a	6.70±0.58 b	2.50±0.51 a
	35~45	100.00±0 a	6.70±0.58 b	1.50±0.71 ab
隋珠	15~25	79.30±1.53 d	3.30±0.58 c	1.00±0.79 bc
	25~35	82.80±0.85 c	3.30±0.58 c	1.00±0.79 bc
	35~45	77.80±2.00 d	0 d	0 c
黔莓1号	15~25	100.00±0 a	3.30±0.58 c	1.00±0.79 bc
	25~35	100.00±0 a	0 d	0 c
	35~45	100.00±0 a	3.30±0.58 c	1.00±0.79 bc
越秀	15~25	100.00±0 a	0 d	0 c
	25~35	93.30±1.15 b	0 d	0 c
	35~45	100.00±0 a	0 d	0 c

品种	叶龄/d	出愈率/%	不定芽诱导率/%	不定芽增殖系数
桃熏	15~25	100.00±0 a	15.60±1.24 a	2.00±0.43 ab
	25~35	100.00±0 a	3.30±0.58 c	1.00±0.79 bc
	35~45	100.00±0 a	6.70±1.15 b	1.50±0.41 ab
圣诞红	15~25	100.00±0 a	14.60±1.15 a	1.50±0.29 ab
	25~35	100.00±0 a	6.70±0.58 b	2.50±0.51 a
	35~45	100.00±0 a	6.70±0.58 b	1.50±0.71 ab
隋珠	15~25	79.30±1.53 d	3.30±0.58 c	1.00±0.79 bc
	25~35	82.80±0.85 c	3.30±0.58 c	1.00±0.79 bc
	35~45	77.80±2.00 d	0 d	0 c
黔莓1号	15~25	100.00±0 a	3.30±0.58 c	1.00±0.79 bc
	25~35	100.00±0 a	0 d	0 c
	35~45	100.00±0 a	3.30±0.58 c	1.00±0.79 bc
越秀	15~25	100.00±0 a	0 d	0 c
	25~35	93.30±1.15 b	0 d	0 c
	35~45	100.00±0 a	0 d	0 c

处理	出愈率	不定芽诱导率
叶背刻伤	84.91±0.57 bc	0 b
叶缘刻伤	55.56±1.53 c	0 b
混合刻伤	100.00±0 abc	3.70 a