浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (9): 2165-2168.DOI: 10.16178/j.issn.0528-9017.20240983

• 果树与蔬菜 • 上一篇    下一篇

花椰菜紫霞65高效制种与遗传纯度鉴定技术

虞慧芳(), 盛小光, 王建升, 沈钰森, 宋蒙飞, 顾宏辉*()   

  1. 浙江省农业科学院 蔬菜研究所,浙江 杭州 310021
  • 收稿日期:2024-12-23 出版日期:2025-09-11 发布日期:2025-10-14
  • 通讯作者: 顾宏辉(1969—),男,研究员,博士,主要从事花椰菜、青花菜品种选育工作,E-mail: guhh@zaas.ac.cn。
  • 作者简介:虞慧芳(1976—),女,副研究员,博士,主要从事花椰菜遗传育种与分子生物学研究工作,E-mail: yuhf@zaas.ac.cn。
  • 基金资助:
    浙江省科技计划重点研发项目(2022C02051-2);浙江省农业(蔬菜新品种选育)新品种选育重大科技专项(2021C02065-4-3)

Efficient seed production and genetic purity identification of Brassica oleracea L.var.botrytis Linnaeus cv. Zixia 65

YU Huifang(), SHENG Xiaoguang, WANG Jiansheng, SHEN Yusen, SONG Mengfei, GU Honghui*()   

  1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang
  • Received:2024-12-23 Online:2025-09-11 Published:2025-10-14

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摘要：

紫霞65是花球紫色的花椰菜新品种。新品种的制种产量和种子纯度直接影响其在生产中的应用推广,因此,研究紫霞65高效制种技术和遗传纯度鉴定技术对该品种的示范推广具有重要意义。本文研究了采用Ogura雄性不育系制种时父母本种植比例和留枝数对紫霞65产量及其相关性状的影响,以及基于大量重测序的单核苷酸多态性(SNP)数据开发的竞争性等位基因特异性PCR(KASP)标记,建立了高通量、低成本的分子标记纯度快速鉴定技术。结果表明,父母本种植比例为2∶3、留1个主枝时,制种产量最高,折合667 m2产量23.3 kg;留2个主枝时,单株枝条数和单株角果数较多,但是每角果粒数较少;开发获得的3个KASP标记S011、S012和S026能对紫霞65杂交种的遗传纯度进行精准鉴定。

关键词: 花椰菜, 紫色, 紫霞65, 制种, 遗传纯度

Abstract:

Zixia 65 is a new variety of Brassica oleracea L. var. botrytis Linnaeus with purple flower heads. The seed production yield and seed purity of a new variety directly affect its application and promotion in production. Therefore, studying the efficient seed production technology and genetic purity identification technology of Zixia 65 is of great significance for the demonstration and promotion of this variety. This study investigated the effects of the planting proportion of paternal lines and the number of branches left on the yield and related traits of Zixia 65 using Ogura male sterile lines. Competitive allele specific PCR (KASP) markers were developed based on a large amount of resequencing single nucleotide polymorphism (SNP) data, and a high-throughput, low-cost molecular marker purity rapid identification technology was developed. The results showed that when the planting proportion of paternal lines was 2∶3 and one main branch was left, the seed yield was the highest, equivalent to 23.3 kg per 667 m2. When two main branches were left, there were more branches and pods per plant, but fewer seeds per pod. Three KASP markers S011, S012 and S026 were developed to accurately identify the genetic purity of the Zixia 65 hybrid.

Key words: Brassica oleracea L. var. botrytis Linnaeus, purple, Zixia 65, seed production, genetic purity

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