玉米bZIP亚家族基因的表达模式分析

doi:10.16178/j.issn.0528-9017.20220211

摘要/Abstract

摘要：

bZIP蛋白是植物转录因子中数目最多、最保守的一类转录因子,参与调控植物生长发育及逆境胁迫响应机制等诸多生命进程。本研究选取玉米bZIP基因亚家族、共计14个ZmbZIP基因为研究对象,系统地研究了ZmbZIP在应答不同逆境胁迫的表达模式。系统进化树分析结果表明,14个ZmbZIP基因可以细分为5个亚组。实时荧光定量PCR(qRT-PCR)分析结果显示,ZmbZIP基因在不同组织器官中有不同的表达模式,其表达模式多样性显示其生物学功能的分化。在人为模拟盐、干旱、低温和硝态氮/铵态氮缺乏等逆境胁迫条件下,ZmbZIP基因呈现不同的表达模式,表明ZmbZIP基因广泛地参与各类逆境胁迫响应途径,并在其中发挥着不同的作用。本研究为将来深入研究这些基因的生物学功能提供科学数据。

关键词: 转录因子, 玉米, 逆境胁迫, 表达模式

中图分类号:

S513

贾利强, 刘洋, 杨胜美, 杨雨娇, 杨秀万. 玉米bZIP亚家族基因的表达模式分析[J]. 浙江农业科学, 2023, 64(6): 1429-1437.

图/表 7

参考文献 39

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基因名称	基因位点	上游引物(5'-3')	下游引物(5'-3')
ZmbZIP5	GRMZM2G062391	TTCCACCCACCCCATCCCAAC	GGCCCCTTCCCAATGTCCAAAC
ZmbZIP12	GRMZM2G332294	CGGAGGTGAAAAGGGCAATGTC	TCAGCGATGTACCTCATCTTCCTTTC
ZmbZIP22	GRMZM2G112483	AGGCTATTTCCGACGCTAAGCTC	AGGCTATTTCCGACGCTAAGCTC
ZmbZIP23	GRMZM5G821024	CCACCACACTTTCGGCACAGC	CTGGACTTCTTCTCTCAGGGCGTC
ZmbZIP31	GRMZM2G180847	ACCACTGAACGCGACCCAA	GACGCCTTTGCCGACGACAC
ZmbZIP35	GRMZM2G060109	GCACCCATCGTATCCCATGCGTG	ATCAACCCCAGGAGACTAACACTTG
ZmbZIP43	GRMZM2G146020	GCAGCAGATTCCATCCTACTTCGTG	ACTGTCTCCCGTTTGATGGACT
ZmbZIP61	AC190609.3_FGT001	CATCACAACCGGCACTCGAT	ATCGATTAGGGCAAGCTCGGCAAG
ZmbZIP62	GRMZM2G149150	CGACACTCTCAGCACAGTTGACAAT	ATCTCCCCAGTTGCGAGTTTCAGAC
ZmbZIP64	GRMZM2G079365	CACGCTTTCGGCACAGCTCAC	GCCGCTGGACTTCTTCCCTC
ZmbZIP105	AC200057.4_FGT007	CCTTGATGTGGACAAGCTGAACT	TGGACTGGCTATGCTGATGCCTTG
ZmbZIP107	GRMZM2G149040	CGGAGAGGTGATGGAGGCCAAGAA	AGTTTGAACCTTCCGCTCCAATTCTG
ZmbZIP130	GRMZM2G151295	CCAATGTGCCAAACCACCAGATG	TGGCCCCTTTCCAATGTCCAAAC
ZmbZIP137	GRMZM2G136266	TCGCGCTCATCGACCCCAAG	AGTGGCCTCCGTTTGCAGAG

基因名称	基因位点	上游引物(5'-3')	下游引物(5'-3')
ZmbZIP5	GRMZM2G062391	TTCCACCCACCCCATCCCAAC	GGCCCCTTCCCAATGTCCAAAC
ZmbZIP12	GRMZM2G332294	CGGAGGTGAAAAGGGCAATGTC	TCAGCGATGTACCTCATCTTCCTTTC
ZmbZIP22	GRMZM2G112483	AGGCTATTTCCGACGCTAAGCTC	AGGCTATTTCCGACGCTAAGCTC
ZmbZIP23	GRMZM5G821024	CCACCACACTTTCGGCACAGC	CTGGACTTCTTCTCTCAGGGCGTC
ZmbZIP31	GRMZM2G180847	ACCACTGAACGCGACCCAA	GACGCCTTTGCCGACGACAC
ZmbZIP35	GRMZM2G060109	GCACCCATCGTATCCCATGCGTG	ATCAACCCCAGGAGACTAACACTTG
ZmbZIP43	GRMZM2G146020	GCAGCAGATTCCATCCTACTTCGTG	ACTGTCTCCCGTTTGATGGACT
ZmbZIP61	AC190609.3_FGT001	CATCACAACCGGCACTCGAT	ATCGATTAGGGCAAGCTCGGCAAG
ZmbZIP62	GRMZM2G149150	CGACACTCTCAGCACAGTTGACAAT	ATCTCCCCAGTTGCGAGTTTCAGAC
ZmbZIP64	GRMZM2G079365	CACGCTTTCGGCACAGCTCAC	GCCGCTGGACTTCTTCCCTC
ZmbZIP105	AC200057.4_FGT007	CCTTGATGTGGACAAGCTGAACT	TGGACTGGCTATGCTGATGCCTTG
ZmbZIP107	GRMZM2G149040	CGGAGAGGTGATGGAGGCCAAGAA	AGTTTGAACCTTCCGCTCCAATTCTG
ZmbZIP130	GRMZM2G151295	CCAATGTGCCAAACCACCAGATG	TGGCCCCTTTCCAATGTCCAAAC
ZmbZIP137	GRMZM2G136266	TCGCGCTCATCGACCCCAAG	AGTGGCCTCCGTTTGCAGAG

基因号	基因名称	大小/aa	分子量/ku	等电点	染色体位置	可变剪切/个
GRMZM2G062391	ZmbZIP5	318	35.10	4.60	Chr1:56911430-56916805	2
GRMZM2G332294	ZmbZIP12	249	26.21	5.06	Chr1:198774832-198777673	1
GRMZM2G112483	ZmbZIP22	229	23.88	5.10	Chr2:34882973-34887627	4
GRMZM5G821024	ZmbZIP23	350	37.78	6.61	Chr2:145114887-145119368	3
GRMZM2G180847	ZmbZIP31	162	17.55	9.32	Chr2:199265525-199270738	4
GRMZM2G060109	ZmbZIP35	362	38.98	7.04	Chr2:223801093-223807824	2
GRMZM2G146020	ZmbZIP43	321	34.63	6.89	Chr3:143644925-143649063	1
AC190 609.3_FGT001	ZmbZIP61	383	40.59	5.00	Chr4:198573893-198576750	1
GRMZM2G149150	ZmbZIP62	466	49.70	7.04	Chr4:199034864-199038928	1
GRMZM2G079365	ZmbZIP64	338	36.34	6.50	Chr4:217387342-217403410	1
AC200 057.4_FGT007	ZmbZIP105	370	39.58	6.58	Chr7:145424990-145430280	1
GRMZM2G149040	ZmbZIP107	374	40.31	7.12	Chr7:177744976-177750636	3
GRMZM2G151295	ZmbZIP130	353	38.05	6.58	Chr9:133377512-133383 097	2
GRMZM2G136266	ZmbZIP137	334	36.01	6.58	Chr10:7855627-7859481	2

基因号	基因名称	大小/aa	分子量/ku	等电点	染色体位置	可变剪切/个
GRMZM2G062391	ZmbZIP5	318	35.10	4.60	Chr1:56911430-56916805	2
GRMZM2G332294	ZmbZIP12	249	26.21	5.06	Chr1:198774832-198777673	1
GRMZM2G112483	ZmbZIP22	229	23.88	5.10	Chr2:34882973-34887627	4
GRMZM5G821024	ZmbZIP23	350	37.78	6.61	Chr2:145114887-145119368	3
GRMZM2G180847	ZmbZIP31	162	17.55	9.32	Chr2:199265525-199270738	4
GRMZM2G060109	ZmbZIP35	362	38.98	7.04	Chr2:223801093-223807824	2
GRMZM2G146020	ZmbZIP43	321	34.63	6.89	Chr3:143644925-143649063	1
AC190 609.3_FGT001	ZmbZIP61	383	40.59	5.00	Chr4:198573893-198576750	1
GRMZM2G149150	ZmbZIP62	466	49.70	7.04	Chr4:199034864-199038928	1
GRMZM2G079365	ZmbZIP64	338	36.34	6.50	Chr4:217387342-217403410	1
AC200 057.4_FGT007	ZmbZIP105	370	39.58	6.58	Chr7:145424990-145430280	1
GRMZM2G149040	ZmbZIP107	374	40.31	7.12	Chr7:177744976-177750636	3
GRMZM2G151295	ZmbZIP130	353	38.05	6.58	Chr9:133377512-133383 097	2
GRMZM2G136266	ZmbZIP137	334	36.01	6.58	Chr10:7855627-7859481	2

玉米bZIP亚家族基因的表达模式分析

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