
浙江农业科学 ›› 2026, Vol. 67 ›› Issue (2): 346-355.DOI: 10.16178/j.issn.0528-9017.20240456
张良1(
), 马文清2, 钟子毓1, 安艳波2, 陆国权2, 崔鹏2(
)
收稿日期:2024-06-03
出版日期:2026-02-28
发布日期:2026-03-07
通讯作者:
崔鹏,E-mail:842130122@qq.com。
作者简介:张良,从事作物学领域研究。E-mail:18858984660@163.com。
ZHANG Liang1(
), MA Wenqing2, ZHONG Ziyu1, AN Yanbo2, LU Guoquan2, CUI Peng2(
)
Received:2024-06-03
Online:2026-02-28
Published:2026-03-07
摘要:
为筛选出不同耐盐性的甘薯材料,本研究选取15个甘薯品种(系)为试验材料,通过测定其抗氧化酶活性、光合参数和相关盐胁迫响应基因表达量等指标,探究盐胁迫对甘薯生理生化的影响。结果表明,在200 mmol·L-1 NaCl处理下,与对照相比,不同甘薯品种(系)的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、Na+/K+均显著增加,净光合速率、蒸腾速率和气孔导度均显著下降,15个品种(系)的IbSOS1、IbNHX1和IbAKT1基因表达量显著高于对照,而IbHKT2;1基因的表达量则呈相反结果。同时,超微结构观察结果显示,盐胁迫下甘薯叶片细胞的细胞壁变薄缺失,线粒体结构损伤严重,叶绿体结构变形,基粒片层减少,内部淀粉粒变大。综合以上结果分析,15个品种(系)的耐盐性可分为3类,第一类包含徐薯18、ZNLSP2017-1-9和ZNLSP2017-5-20等3个耐盐品种(系);第二类包含ZNLSP2017-1-8、ZNLSP2017-1-12、ZNLSP2017-1-16、ZNLSP2017-1-39、ZNLSP2017-2-10、ZNLSP2017-5-11、心香、浙薯132和浙薯75等9个中等耐盐品种(系);第三类包含ZNLSP2017-2-12、宁紫薯8号和浙紫薯3号等3个不耐盐品种(系)。本研究可为甘薯耐盐新品种的选育提供种质资源,并为甘薯苗期耐盐性评价提供理论依据。
中图分类号:
张良, 马文清, 钟子毓, 安艳波, 陆国权, 崔鹏. 甘薯苗期耐盐性筛选与综合评价[J]. 浙江农业科学, 2026, 67(2): 346-355.
ZHANG Liang, MA Wenqing, ZHONG Ziyu, AN Yanbo, LU Guoquan, CUI Peng. Screening and comprehensive evaluation of salt tolerance in sweetpotato during seedling stage[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(2): 346-355.
| 引物名称 | 引物序列(5'?3') |
|---|---|
| α-Tubulin-F | CAACTACCAGCCACCAACTGT |
| α-Tubulin-R | CAAGATCCTCACGAGCTTCAC |
| IbHKT2;1-F | TGCATTCATCACTGAGAGGAG |
| IbHKT2;1-R | GGTGCAGTTTCTGCAACCTC |
| IbLti6 a-F | CCTTCCAAGGTGATGGTGAA |
| IbLti6 a-R | CCGTCCAAAGAACCAGAAAA |
| IbSOS1-F | ATACTGAGTGGGGTTGTTATTGC |
| IbSOS1-R | AAAGGTAAATTTCAAAAGGTACATGG |
| IbNHX1-F | AATGATCACCAGCACCATCA |
| IbNHX1-R | AAGGCTCAGAGGTGACAGGA |
| IbAKT1-F | GAAACGAGCAATGCGTCAG |
| IbAKT1-R | CTTCTCACACAGCGCTTCC |
表1 基因引物序列
Table 1 Primer sequence of genes
| 引物名称 | 引物序列(5'?3') |
|---|---|
| α-Tubulin-F | CAACTACCAGCCACCAACTGT |
| α-Tubulin-R | CAAGATCCTCACGAGCTTCAC |
| IbHKT2;1-F | TGCATTCATCACTGAGAGGAG |
| IbHKT2;1-R | GGTGCAGTTTCTGCAACCTC |
| IbLti6 a-F | CCTTCCAAGGTGATGGTGAA |
| IbLti6 a-R | CCGTCCAAAGAACCAGAAAA |
| IbSOS1-F | ATACTGAGTGGGGTTGTTATTGC |
| IbSOS1-R | AAAGGTAAATTTCAAAAGGTACATGG |
| IbNHX1-F | AATGATCACCAGCACCATCA |
| IbNHX1-R | AAGGCTCAGAGGTGACAGGA |
| IbAKT1-F | GAAACGAGCAATGCGTCAG |
| IbAKT1-R | CTTCTCACACAGCGCTTCC |
图1 盐胁迫对不同甘薯品种(系)叶片SOD活性的影响SOD活性以鲜重计,柱上无相同小写字母代表差异显著(p<0.05),图2~4同。
Fig.1 Effect of salt stress on SOD activities in leaves of different sweetpotato varieties
| 品种/系 | 处理 | 净光合速率/(μmol·m-2·s-1) | 蒸腾速率/(mmol·m-2·s-1) | 气孔导度/(mmol·m-2·s-1) |
|---|---|---|---|---|
| ZNLSP2017-1-8 | CK | 6.5 ± 0.3 a | 2.7 ± 0.4 a | 172.5 ± 16.4 a |
| 200 mmol·L-1 NaCl | 2.2 ± 0.2 b | 0.8 ± 0.2 c | 55.6 ± 4.9 b | |
| ZNLSP2017-1-9 | CK | 8.3 ± 0.4 a | 2.5 ± 0.3 a | 170.6 ± 18.2 a |
| 200 mmol·L-1 NaCl | 2.6 ± 0.3 b | 1.3 ± 0.1 b | 60.3 ± 5.2 b | |
| ZNLSP2017-1-12 | CK | 6.9 ± 0.3 a | 2.7 ± 0.2 a | 173.2 ± 19.1 a |
| 200 mmol·L-1 NaCl | 2.5 ± 0.2 b | 0.9 ± 0.2 b | 56.2 ± 4.5 b | |
| ZNLSP2017-1-16 | CK | 7.1 ± 0.3 a | 2.6 ± 0.3 a | 170.1 ± 18.4 a |
| 200 mmol·L-1 NaCl | 2.3 ± 0.2 b | 0.9 ± 0.1 b | 57.0 ± 5.2 b | |
| ZNLSP2017-1-39 | CK | 6.9 ± 0.3 a | 2.5 ± 0.4 a | 172.8 ± 18.6 a |
| 200 mmol·L-1 NaCl | 2.1 ± 0.2 b | 0.7 ± 0.3 c | 55.1 ± 4.8 b | |
| ZNLSP2017-2-12 | CK | 7.3 ± 0.4 a | 2.6 ± 0.2 a | 168.5 ± 17.3 a |
| 200 mmol·L-1 NaCl | 1.8 ± 0.3 c | 0.5 ± 0.1 c | 47.4 ± 4.9 c | |
| ZNLSP2017-2-10 | CK | 7.5 ± 0.5 a | 2.7 ± 0.2 a | 169.3 ± 17.9 a |
| 200 mmol·L-1 NaCl | 2.4 ± 0.2 b | 1.1 ± 0.2 b | 52.7 ± 4.3 c | |
| ZNLSP2017-5-11 | CK | 7.6 ± 0.4 a | 2.8 ± 0.4 a | 167.4 ± 19.5 a |
| 200 mmol·L-1 NaCl | 2.3 ± 0.3 b | 1.2 ± 0.2 b | 54.5 ± 3.9 b | |
| ZNLSP2017-5-20 | CK | 6.8 ± 0.6 a | 3.1 ± 0.3 a | 168.9 ± 18.2 a |
| 200 mmol·L-1 NaCl | 2.2 ± 0.1 b | 1.4 ± 0.1 b | 59.7 ± 5.1 b | |
| 徐薯18 | CK | 7.2 ± 0.4 a | 2.6 ± 0.3 a | 171.2 ± 19.3 a |
| 200 mmol·L-1 NaCl | 2.4 ± 0.2 b | 1.0 ± 0.1 b | 58.2 ± 5.3 b | |
| 心香 | CK | 6.4 ± 0.8 a | 2.5 ± 0.2 a | 169 ± 17.1 a |
| 200 mmol·L-1 NaCl | 2.1 ± 0.1 b | 1.1 ± 0.1 b | 52.3 ± 5.2 c | |
| 浙薯132 | CK | 6.8 ± 0.6 a | 2.6 ± 0.3 a | 172.6 ± 17.3 a |
| 200 mmol·L-1 NaCl | 2.0 ± 0.2 b | 0.8 ± 0.2 c | 51.1 ± 4.7 c | |
| 浙薯75 | CK | 7.8 ± 0.7 a | 2.5 ± 0.4 a | 173.8 ± 16.9 a |
| 200 mmol·L-1 NaCl | 2.3 ± 0.2 b | 1.2 ± 0.2 b | 52.8 ± 4.6 c | |
| 宁紫薯8号 | CK | 6.9 ± 0.7 a | 2.7 ± 0.3 a | 170.5 ± 18.1 a |
| 200 mmol·L-1 NaCl | 1.7 ± 0.1 c | 0.6 ± 0.1 c | 48.1 ± 5.6 c | |
| 浙紫薯3号 | CK | 7.4 ± 0.7 a | 2.8 ± 0.3 a | 172.4 ± 19.2 a |
| 200 mmol·L-1 NaCl | 1.8 ± 0.2 c | 0.6 ± 0.2 c | 48.8 ± 5.3 c |
表2 盐胁迫下不同甘薯品种(系)叶片的光合参数变化
Table 2 Changes of photosynthetic parameters in leaves of different sweetpotato varieties under salt stress
| 品种/系 | 处理 | 净光合速率/(μmol·m-2·s-1) | 蒸腾速率/(mmol·m-2·s-1) | 气孔导度/(mmol·m-2·s-1) |
|---|---|---|---|---|
| ZNLSP2017-1-8 | CK | 6.5 ± 0.3 a | 2.7 ± 0.4 a | 172.5 ± 16.4 a |
| 200 mmol·L-1 NaCl | 2.2 ± 0.2 b | 0.8 ± 0.2 c | 55.6 ± 4.9 b | |
| ZNLSP2017-1-9 | CK | 8.3 ± 0.4 a | 2.5 ± 0.3 a | 170.6 ± 18.2 a |
| 200 mmol·L-1 NaCl | 2.6 ± 0.3 b | 1.3 ± 0.1 b | 60.3 ± 5.2 b | |
| ZNLSP2017-1-12 | CK | 6.9 ± 0.3 a | 2.7 ± 0.2 a | 173.2 ± 19.1 a |
| 200 mmol·L-1 NaCl | 2.5 ± 0.2 b | 0.9 ± 0.2 b | 56.2 ± 4.5 b | |
| ZNLSP2017-1-16 | CK | 7.1 ± 0.3 a | 2.6 ± 0.3 a | 170.1 ± 18.4 a |
| 200 mmol·L-1 NaCl | 2.3 ± 0.2 b | 0.9 ± 0.1 b | 57.0 ± 5.2 b | |
| ZNLSP2017-1-39 | CK | 6.9 ± 0.3 a | 2.5 ± 0.4 a | 172.8 ± 18.6 a |
| 200 mmol·L-1 NaCl | 2.1 ± 0.2 b | 0.7 ± 0.3 c | 55.1 ± 4.8 b | |
| ZNLSP2017-2-12 | CK | 7.3 ± 0.4 a | 2.6 ± 0.2 a | 168.5 ± 17.3 a |
| 200 mmol·L-1 NaCl | 1.8 ± 0.3 c | 0.5 ± 0.1 c | 47.4 ± 4.9 c | |
| ZNLSP2017-2-10 | CK | 7.5 ± 0.5 a | 2.7 ± 0.2 a | 169.3 ± 17.9 a |
| 200 mmol·L-1 NaCl | 2.4 ± 0.2 b | 1.1 ± 0.2 b | 52.7 ± 4.3 c | |
| ZNLSP2017-5-11 | CK | 7.6 ± 0.4 a | 2.8 ± 0.4 a | 167.4 ± 19.5 a |
| 200 mmol·L-1 NaCl | 2.3 ± 0.3 b | 1.2 ± 0.2 b | 54.5 ± 3.9 b | |
| ZNLSP2017-5-20 | CK | 6.8 ± 0.6 a | 3.1 ± 0.3 a | 168.9 ± 18.2 a |
| 200 mmol·L-1 NaCl | 2.2 ± 0.1 b | 1.4 ± 0.1 b | 59.7 ± 5.1 b | |
| 徐薯18 | CK | 7.2 ± 0.4 a | 2.6 ± 0.3 a | 171.2 ± 19.3 a |
| 200 mmol·L-1 NaCl | 2.4 ± 0.2 b | 1.0 ± 0.1 b | 58.2 ± 5.3 b | |
| 心香 | CK | 6.4 ± 0.8 a | 2.5 ± 0.2 a | 169 ± 17.1 a |
| 200 mmol·L-1 NaCl | 2.1 ± 0.1 b | 1.1 ± 0.1 b | 52.3 ± 5.2 c | |
| 浙薯132 | CK | 6.8 ± 0.6 a | 2.6 ± 0.3 a | 172.6 ± 17.3 a |
| 200 mmol·L-1 NaCl | 2.0 ± 0.2 b | 0.8 ± 0.2 c | 51.1 ± 4.7 c | |
| 浙薯75 | CK | 7.8 ± 0.7 a | 2.5 ± 0.4 a | 173.8 ± 16.9 a |
| 200 mmol·L-1 NaCl | 2.3 ± 0.2 b | 1.2 ± 0.2 b | 52.8 ± 4.6 c | |
| 宁紫薯8号 | CK | 6.9 ± 0.7 a | 2.7 ± 0.3 a | 170.5 ± 18.1 a |
| 200 mmol·L-1 NaCl | 1.7 ± 0.1 c | 0.6 ± 0.1 c | 48.1 ± 5.6 c | |
| 浙紫薯3号 | CK | 7.4 ± 0.7 a | 2.8 ± 0.3 a | 172.4 ± 19.2 a |
| 200 mmol·L-1 NaCl | 1.8 ± 0.2 c | 0.6 ± 0.2 c | 48.8 ± 5.3 c |
图5 200 mmol·L-1 NaCl处理前(A)后(B)甘薯叶片超微结构的变化(以浙紫薯3号为例)Thy,胸腺;MC,线粒体;CW,细胞壁;CM,细胞膜;Chl,叶绿体;P,质体;SG,淀粉粒。
Fig. 5 Electron micrographs of leaf cells in sweetpotato under CK(A) and 200 mmol·L-1 NaCl treatment(B)(Zhezishu 3)
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