Construction of DNA fingerprinting of vegetable legumes using core-SNP markers

doi:10.16178/j.issn.0528-9017.20240558

Abstract

Abstract:

Vegetable legumes are important vegetable crops in China. To facilitate the variety protection of vegetable legumes, this study focused on cowpea and vegetable pea as research objects, and constructed the DNA fingerprinting maps of 52 cowpea varieties(lines) and 48 pea germplasm using the species-specific core single nucleotide polymorphism(SNP) markers, respectively. We also evaluated the seed purity for 14 cowpea varieties(lines). Our results showed that SNP markers based on the kompetitive allele-specific PCR(KASP) technology could be used in protecting the new variety rights, distinguishing the variety authenticity and evaluating the seed purity. Meanwhile, we also suggested that we should formulate and publish the technical standards for identifying the vegetable legumes varieties based on SNP markers as soon as possible, which will provide the technical support for the healthy development of seed industry in vegetable legumes.

Key words: vegetable legumes, SNP marker, KASP, variety protection, seed purity

CLC Number:

S643

WU Xinyi, LIU Na, WANG Baogen, GONG Yaming, LI Guojing. Construction of DNA fingerprinting of vegetable legumes using core-SNP markers[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(2): 363-369.

Figures/Tables 8

References 17

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编号	品种	编号	品种	编号	品种	编号	品种
1	CFDY	14	ZJXX	27	2022_11	40	HZ_1
2	CH	15	4_C41	28	2022_12	41	HZ_2
3	CSSL	16	5_C25	29	2022_13	42	HZ_4
4	DFL	17	2022_1	30	2022_14	43	MZ_1
5	HL	18	2022_2	31	2022_15	44	MZ_2
6	SF620	19	2022_3	32	2022_16	45	MZ_4
7	JTYY	20	2022_4	33	2022_17	46	R153
8	LJ	21	2022_5	34	2022_18	47	TZ30
9	LXH	22	2022_6	35	2022_19	48	ZJ60
10	LZTL	23	2022_7	36	2022_20	49	ZJ106
11	MGTX	24	2022_8	37	G98	50	ZJ618
12	YQ18	25	2022_9	38	G323	51	ZJ633
13	YYQF	26	2022_10	39	H18	52	ZN016

编号	品种	编号	品种	编号	品种	编号	品种
1	CFDY	14	ZJXX	27	2022_11	40	HZ_1
2	CH	15	4_C41	28	2022_12	41	HZ_2
3	CSSL	16	5_C25	29	2022_13	42	HZ_4
4	DFL	17	2022_1	30	2022_14	43	MZ_1
5	HL	18	2022_2	31	2022_15	44	MZ_2
6	SF620	19	2022_3	32	2022_16	45	MZ_4
7	JTYY	20	2022_4	33	2022_17	46	R153
8	LJ	21	2022_5	34	2022_18	47	TZ30
9	LXH	22	2022_6	35	2022_19	48	ZJ60
10	LZTL	23	2022_7	36	2022_20	49	ZJ106
11	MGTX	24	2022_8	37	G98	50	ZJ618
12	YQ18	25	2022_9	38	G323	51	ZJ633
13	YYQF	26	2022_10	39	H18	52	ZN016

编号	品种	编号	品种	编号	品种	编号	品种
1	ZW009	13	31	25	44	37	67
2	ZW4	14	J16	26	46	38	68
3	CZ	15	33	27	MZ	39	CSR
4	ZW9	16	Ku	28	48	40	SQSM
5	19	17	36	29	49	41	DWX
6	ZW10	18	J14	30	JP2	42	72
7	CXW	19	38	31	51	43	ZW2
8	KSKT	20	J210	32	54	44	74
9	523	21	40	33	EO	45	3303
10	51	22	41	34	Onward	46	76
11	750	23	GS26	35	61	47	77
12	J29	24	43	36	66	48	3320

编号	品种	编号	品种	编号	品种	编号	品种
1	ZW009	13	31	25	44	37	67
2	ZW4	14	J16	26	46	38	68
3	CZ	15	33	27	MZ	39	CSR
4	ZW9	16	Ku	28	48	40	SQSM
5	19	17	36	29	49	41	DWX
6	ZW10	18	J14	30	JP2	42	72
7	CXW	19	38	31	51	43	ZW2
8	KSKT	20	J210	32	54	44	74
9	523	21	40	33	EO	45	3303
10	51	22	41	34	Onward	46	76
11	750	23	GS26	35	61	47	77
12	J29	24	43	36	66	48	3320

品种	DNA指纹图谱
CFDY	TCGGCTCCTTGTTCTGCCGAAGTAGCTTTTCTTAGCGCGCATTACGGGAT
CH	TCGGCTACTTGTTCTGCTTAAGCAGTGTTTCTTAGCGTCCGTTACGGGAG
CSSL	TCGGCTACTTGTTCTAATGAAGTAGCGTTTCTTAGCGTCCATTACGGGAT
DFL	TCGGCTACTTGTTCTAATGAAGTAGCGTTTCTTAGCGTCCATTACGGGAT
HL	TCGGTTACTTGGGTTACTGAATTAGCGCTCCTTAGCGTGCACTACGGGAT
SF620	TCGGTTATTTGTTCTACTGAATTAGCGTTTCTTAGCGTCCATTACGGGAT
JTYY	TCGGTTACTTGTTCTGCTTAAGTGGCGTTTCCTAGCGTGCATTACGGGAT
LJ	TCAGCTACT-GTTCTAATTAAGCGGCTTTTACTAGCGCCCATTACGGGAT
LXH	GAAGCTATTCGTTCCAATGAAGTAGTGTTTATCAGCGCCCGCTGCGGGAT
LZTL	TCGGTTACTTGTTCTGCTGAAGTGGCTTTTCCTAGCGTGCATTACGGGAT
MGTX	KCGGTTACTTGTTCTACTGAAGTGGTTTTTCCTAGAGTGCGTTACGGGAT
YQ18	GCGGTTACTTGTTTTGCTGGGTTAGCGCTTATCAGCGTCCATTACGGGAG
YYQF	TCGGTTACTTGTTCTACTGAAGTGGCTTTTCCTAGAGTGCATTACGGGAT
ZJXX	TCGGTTACTTGTTCTACTGAAGTGGCTTTTCCTAGAGTGCATTACGGGAT
4_C41	GCAATTATTTGGTTTACYTGGTCGGTTCTTACCAGAGTGCGTTACGGGAG
5_C25	TCAGCTACTTGTTCTAATTAAGCGGCTTTTACTAGCGCCCATTACGGGAT
2022_1	TCGATTCTTTATTTTGCTTGGGCGGTGTTTACTAGCGCGCGCTACGGGAG
2022_2	TCGGCTACTTGTTCTAATTAAGTAGCGTTTCTTAGCGTCCATTGCGGGAT
2022_3	TCGGCTACTTGTTCTAATGAAGTAGCGTTTCTTAGCGTCCATTACGGGAT
2022_4	TCAGTTCCTTGTTCTACTGAAGTAGTGTTTCTTAGCGTGCGTTACGGGAT
2022_5		TCAGCTACTTGTTCTAATTAAGCGGCTTTTACTAGCGCCCATTACGGGAT
2022_6		TCGGTTACTTGTTCTACTGAAGTGGTTTTTCCTAGAGTGCGTTACGGGAT
2022_7		TCGGTTCTTTGTTTTGCTTGGGCGGTGTTTACTAGCGCCCGCTACGGGAG
2022_8		TCGGCTACTTGTTCTACTTAAGCAGTGTTTCTTAGCGTCCGTTACGGGAG
2022_9		TAAATTCTTTATTTTGATTGAGTGGCGTTTCTTAGCGCCCACTGCGGGAG
2022_10		TCGGTTCTTTGTTTTACTTGGGCGGTGTTTACCAGCGCGCGTTACGGGAG
2022_11		TCGGTTACTTGTTCTACTGAAGTGGTTTTTCCTAGAGTGCGTTACGGGAT
2022_12		TCAACTCTTTGGTTTGCTTGAGCGATGTCTCCTAGCGCCCGCTGCGGGAG
2022_13		TCGGTTCTTTGTTCTGCTTGGGCGGTGTTTACCAGCGCCCGTTGCGGGAG
2022_14		TCGGTTCCTTGTTTTGCTGGATTGGCTTTCCCTAGCGCCCATTACGGGAT
2022_15		TCGGTTCTTTGTTTTACTGGGGCGGTGTTTACTAGCGCGCGTTACGGGAT
2022_16		TCGGTTACTTGTTCTGCCGAAGTGGCGTTTCCTAGAGCGCATTACGGGAT
2022_17		GAGGTTCTTTGTTTTACTTAATCGGTGTTTACCAGCGTGCGTTACGGGAG
2022_18		GAAACTCTTCGGTCCAACTAGTTAGTTCTCATCAGCGCCCGCTGCGGGAG
2022_19		TCGGTTACTTGTTCTGCTTAATTGGCTCTTCCTAGCGTGCATTACGGGAT
2022_20		TCGGTTACTTGTTCTGCTGAAGTAGCGTTTCTTAGAGTGCATTACGGGAT
G98		TAAATTATTTGTTTCAACGGAGTGGTTTTTCCTAGCGCCCACTAAGGGAG
G323		GCAGTTCCTCAGTTCAACTGATTAATTCTTCTTCTCATCTGTTAACGAAG
H18		TCAGCTACTTGTTCTAATTAAGCGGCTTTTACTAGCGCCCATTACGGGAT
HZ_1		KCGATTCTTTATTTTGCTTGGGCGGTGT-TACTAGCGCGCGCTACGGGAG
HZ_2		KCGATTCTTTATTTTGCTTGGGCGGTGTTTACTAGCGCGCGCTACGGGAG
HZ_4		KCGATTCTTTATTTTGCTTGGGCGGTGTTTACTAGCGCGCGCTACGGGAG
MZ_1		TCGGTTACTTGTTCTACTGAAGTGGTTTTTCCTAGAGTGCGTTACGGGAT
MZ_2		KCGGTTACTTGTTCTACTGAAGTGGTTTTTCCTAGAGTGCGTTACGGGAT
MZ_4		TCGATTCTTTATTTTGCTTGGGCGGTGTTTACTAGCGCGCGCTACGGGAG
R153		GMGATTATTCGTGCCGCCTGGGCAGTGTCTCTCATAATCTACGGAGGAGT
TZ30		TCGGCTCTTTGGTCCGCCTGGGCGATGTTCACCAGAGCCCGTTGCGGGAG
ZJ60		TAGGTTCTTTGTGTTGCTTAAGTGGTGTTTACCAGAGTCCGCTACGGGAG
ZJ106		TCGGCTACTTGTTCTAATGAAGTAGCGTTTCTTAGCGTCCATTACGGGAT
ZJ618		TCGGTTACTTGTTCTGCTTAATTGGCTCTTCCTAGCGTGCATTACGGGAT
ZJ633		TCGGTTACTTGTTCTGCTGAAGTGGCTTTTCCTAGCGTGCATTACGGGAT
ZN016		GAAATCATTTGTGTTAATTAATTAGTTCTTCTTATCATGCACGGACGAGT