Journal of Zhejiang Agricultural Sciences

Journal of Zhejiang Agricultural Sciences ›› 2025, Vol. 66 ›› Issue (10): 2363-2367.DOI: 10.16178/j.issn.0528-9017.20240826

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Effect of wheat bran addition amount on the growth and active ingredients of Ganoderma lingzhi during substitute cultivation

ZENG Fanqing1(), LYU Mingliang1, FENG Na2   

  1. 1. Lishui Institute of Agriculture and Forestry Sciences, Lishui 323000, Zhejiang
    2. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungal Resources and Utilization (South) of Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403
  • Received:2024-10-28 Online:2025-10-11 Published:2025-10-21

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Abstract:

This study cultivated Ganoderma lingzhi using a substitute cultivation method and compared various formulas with 0, 5%, 10%, 20%, and 30% wheat bran addition to examine the impact of the carbon/nitrogen ratio on the growth rate of myceliam, biological efficency, and active ingredient content. The results showed that when the wheat bran addition amount was 0, the harvested fruiting bodies of G. lingzhi had the highest polysaccharide content. When the wheat bran addition amount was 5%, the total content of triterpenoid in both the fruiting bodies and spores of G. lingzhi was the highest, and the polysaccharide content in the spores was also at its peak. The biological efficency of G. lingzhi reached the highest (25.53%) when the wheat bran addition amount was 20%. The study indicates that an appropriate carbon/nitrogen ratio is essential for ensuring the production efficiency and quality of G. lingzhi.

Key words: substitute cultivation, Ganoderma lingzhi, wheat bran, biomass, polysaccharide, triterpenoid

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