Application of biochar-based immobilization of deltamethrin-degrading bacteria

doi:10.16178/j.issn.0528-9017.20250060

Abstract

Abstract:

In view of the deltamethrin contamination in soil, the biocarbon produced by Photinia fraseri was used as the fixed material to prepare the immobilized deltamethrin-degrading bactericide from bacteria Aspergillus niger, and the preparation conditions were optimized. On this basis, the stability of the immobilized bactericide and the effect of degrading deltamethrin were evaluated. The results showed that when the concentration of biochar was 20 mg·mL^-1, the inoculation amount of Aspergillus niger was 5%, the immobilization temperature was 30 ℃, and the immobilization time was 24 h, the degradation rate of deltamethrin by the immobilized bactericide was as high as 90.12%, and after the immobilized bactericide was stored at 4 ℃ and 25 ℃ for 28 days, the degradation rates could still be maintained at 77.46% and 69.72%. The results showed that the immobilized bactericide had good stability in degradation of deltamethrin,which provided a good bioremediation material for solving the degradation of deltamethrin in soil.

Key words: biochar, deltamethrin, Aspergillus niger, immobilization

CLC Number:

S482

JIANG Hongying, ZHANG Zhenwu, XING Chenghua. Application of biochar-based immobilization of deltamethrin-degrading bacteria[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2493-2497.

Figures/Tables 7

Table 1 Optimization of preparation conditions of biochar-based immobilized bactericide

因素	水平
生物质炭浓度/(mg·mL^-1)	5	10	20	30	40
黑曲霉菌接种量/%(V/V)	1	3	5	10	20
固定化时间/h	12	24	36	48	72
固定化温度/℃	15	20	25	30	35

Fig.1 Effect of different biochar concentrations on degradation rate of deltamethrin

Fig.2 Effect of different inoculation amounts of Aspergillus niger on degradation rate of deltamethrin

Fig.3 Effect of different immobilization temperatures on degradation rate of deltamethrin

Fig.4 Effect of different immobilization time on degradation rate of deltamethrin

Fig.5 Effect of storage time of immobilized bactericide on degradation rate of deltamethrin at 4 ℃

Fig.6 Effect of storage time of immobilized bactericide on deltamethrin degradation rate at 25 ℃

References 20

[1]	AKOIJAM R, NINGOMBAM A, BEEMROTE A, et al. Residual pattern of chlorantraniliprole, thiamethoxam, flubendiamide and deltamethrin in tomato fruit and soil[J]. Bulletin of Environmental Contamination and Toxicology, 2023, 111(6): 69.
[2]	JOKAR S H, SHAVANDI M, HADDADI A, et al. Remediation of chlorpyrifos and deltamethrin in different salinity soils and its impact on soil bacterial composition[J]. International Journal of Environmental Science and Technology, 2022, 19(12):12057-12068.
[3]	倪冲, 周虹. 异位热脱附技术在农药污染土壤的应用研究[J]. 广东化工, 2022, 49(11): 141-145.
[4]	陈俊华, 李绍华, 刘晋恺, 等. 燃气热脱附技术土壤修复效果及影响因素[J]. 环境工程学报, 2022, 16(5):1610-1619.
[5]	张桐, 张展华, 胡杰华, 等. 淋洗技术在土壤污染修复中的应用与挑战[J]. 环境化学, 2022, 41(11):3599-3612.
[6]	VITOLA G, MAZZEI R, GIORNO L. Biohybrid membranes for organophosphate pesticides degradation: Hyperactivation of immobilized phosphotriesterase by surfactants[J]. Environmental Technology & Innovation, 2023,30: 103053.
[7]	吴秀, 王娴, 余亚韩, 等. 固定化黑曲霉降解3-苯氧基苯甲酸的作用途径及对溴氰菊酯废水的修复效果[J]. 环境科学学报, 2025, 45(3): 123-134.
[8]	ETSHINDO L A, SOUSA C, TAMIASSO-MARTINHON P, et al. SnO₂-TiO₂ materials for photocatalytic degradation of cationic dye under UV and visible light and a chitosan composite film investigation[J]. Catalysis Today, 2025, 444: 114995.
[9]	MOHAPATRA D, RATH S K, MOHAPATRA P K. Accelerated degradation of four organophosphorus insecticides by malathion tolerant Aspergillus niger MRU01 a soil fungus[J]. Geomicrobiology Journal, 2024, 41(4):474-483.
[10]	RUAN Z P, XU M J, XING Y W, et al. Enhanced growth of wheat in contaminated fields via synthetic microbiome as revealed by genome-scale metabolic modeling[J]. The Science of The Total Environment, 2024, 953:176047.
[11]	ZHOU X R, LAI C, ALMATRAFI E, et al. Unveiling the roles of dissolved organic matters derived from different biochar in biochar/persulfate system: mechanism and toxicity[J]. Science of The Total Environment, 2023, 864:161062.
[12]	ZHANG D H, LIU Z M. Employing dolomite as magnesium source to prepare calcined layered double hydroxides for chromium contaminated soil treatment: exploring the influence of temperature, bioavailability, and microbial diversity[J]. Heliyon, 2024, 10(15): e34664.
[13]	MENG J J, DI Y L, GENG Y T, et al. Enhanced nitrate removal efficiency and microbial response of immobilized mixed aerobic denitrifying bacteria through biochar coupled with inorganic electron donors in oligotrophic water[J]. Bioresource Technology, 2024,396: 130457.
[14]	ZUO X Z, LU W Y, LING W T, et al. Biodegradation of PAEs in contaminated soil by immobilized bacterial agent and the response of indigenous bacterial community[J]. Environmental Pollution, 2024, 361:124925.
[15]	PENG F, LIU J S, PING J P, et al. An effective strategy for biodegradation of high concentration phenol in soil via biochar-immobilized Rhodococcus pyridinivorans B403[J]. Environmental Science and Pollution Research, 2024, 31(23):33752-33762.
[16]	Chandi K, Udomkun P, Boonupara T, et al. Enhancing soil health, microbial count, and hydrophilic methomyl and hydrophobic lambda-cyhalothrin remediation with biochar and nano-biochar[J]. Scientific Reports, 2024,14: 19551.
[17]	JI Y H, CAO Y Z, WANG Y, et al. Effects of adding lignocellulose-degrading microbial agents and biochar on nitrogen metabolism and microbial community succession during pig manure composting[J]. Environmental Research, 2023,239: 117400.
[18]	王莹, 林凤翔, 岳星雨, 等. 生物炭固定SM3菌剂的制备及其对三唑酮的降解研究[J]. 农业环境科学学报, 2022, 41(12):2604-2612.
[19]	THI MAI N, VAN THANH D, NHAT HUY N, et al. Red mud supported on rice husk biochar as sono-photo-Fenton catalysts for degradation of ciprofloxacin in water[J]. Separation and Purification Technology, 2025, 354: 129039.
[20]	张晓菲, 倪晓菁, 张子仪, 等. 响应面法优化黑曲霉固定化条件及其对土壤中溴氰菊酯的降解特性研究[J]. 微生物学报, 2023, 63(12):4574-4593.