Application of immobilized microorganism technology on soil remediation of farmland in Xiaoqinling gold mining area

doi:10.16178/j.issn.0528-9017.20230614

Abstract

Abstract:

In order to explore new methods of soil pollution prevention, the application of immobilized microorganism technology in soil ecological remediation was studied. The heavy metal contents in the soil of Xiaoqinling gold mining area and the surrounding farmland in Western Henan Province were determined and evaluated, it was found that the risk of Pb²⁺ element pollution in the soil in this area was high. The mixed bacteria of Bacillus laterosporus, actinomycetes and yeast were immobilized to carry out soil bioremediation in situ, the results showed that the urease activities of soil treated with immobilized bacteria were significantly higher than that of CK. In order to improve the passivation efficiency and removal rate of Pb²⁺, the single factor test and response surface methodology were used to analyze the factors affecting the soil remediation effect, the optimal removal conditions of Pb²⁺ were determined finally: the dosage of immobilized mixed bacteria was 10.496%, the treatment temperature was 30.784 ℃, the pH value was 6.242, and the theoretical removal rate of Pb²⁺ was 72.754%. The model was verified by experiment, the result of experiment was close to the theoretical value, indicating that response surface analysis can optimize the soil remediation experimental parameters.

Key words: immobilized microorganism technology, soil in-situ remediation, removal rate, single factor test, response surface methodology

CLC Number:

LI Lijun, ZHONG Wei, LI Tao. Application of immobilized microorganism technology on soil remediation of farmland in Xiaoqinling gold mining area[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(8): 1950-1956.

Figures/Tables 11

References 20

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主要参数	Hg	As	Pb	Cr	Cu	Zn
最小值	0.04	5.08	21.20	40.24	13.40	44.85
最大值	42.57	13.62	3 654.06	82.13	872.60	572.81
平均值	3.36	8.16	303.89	60.23	82.23	89.81
标准差	11.78	2.74	1 006.60	15.54	237.49	145.18
变异系数	3.50	0.34	3.31	0.26	2.89	1.62
周边土壤对照值	0.08	8.19	32.62	55.99	23.93	68.74
GB 15168-2018限值	3.40	25.00	170.00	250.00	100.00	300.00

主要参数	Hg	As	Pb	Cr	Cu	Zn
最小值	0.04	5.08	21.20	40.24	13.40	44.85
最大值	42.57	13.62	3 654.06	82.13	872.60	572.81
平均值	3.36	8.16	303.89	60.23	82.23	89.81
标准差	11.78	2.74	1 006.60	15.54	237.49	145.18
变异系数	3.50	0.34	3.31	0.26	2.89	1.62
周边土壤对照值	0.08	8.19	32.62	55.99	23.93	68.74
GB 15168-2018限值	3.40	25.00	170.00	250.00	100.00	300.00

评价指标	Hg	As	Pb	Cr	Cu	Zn
P_i	0.989	0.326	1.788	0.241	0.822	0.299
P_综合	8.881	0.449	15.251	0.288	6.198	1.367

评价指标	Hg	As	Pb	Cr	Cu	Zn
P_i	0.989	0.326	1.788	0.241	0.822	0.299
P_综合	8.881	0.449	15.251	0.288	6.198	1.367

项目	平方和	自由度	均方	F值	显著性
Model	3 478.7	9	386.52	78.56	^**
A	80.39	1	80.39	16.34	^**
B	122.23	1	122.23	24.84	^**
C	67.69	1	67.69	13.76	^**
AB	2.18	1	2.18	0.44
AC	4.73	1	4.73	0.96
BC	123.88	1	123.88	25.18	^**
A²	907.32	1	907.32	184.40	^**
B²	870.31	1	870.31	176.88	^**
C²	976.26	1	976.26	198.41	^**
Residual	34.44	7	4.92
Lack of Fit	15.08	3	5.03	1.04
Pure Error	19.36	4	4.84
Cor Total	3 513.14	16