Diagnosis of drought stress in lilies based on chlorophyll fluorescence parameters

doi:10.16178/j.issn.0528-9017.20240944

Abstract

Abstract:

Water and photosynthesis are key factors regulating plant growth，and drought stress induces stomatal closure in leaves，thereby impairing plant photosynthetic performance. To clarify the changes in chlorophyll fluorescence parameters of lily ‘Sunderland’ under drought stress，this study analyzed chlorophyll fluorescence parameters under varying photosynthetically active radiations and temperatures，and calibrated the functional relationship between photosynthetic rate and chlorophyll fluorescence parameters. Additionally，data collected during drought stress and after stress relief were used to examine changes in chlorophyll fluorescence parameters before and after rewatering. The results showed that with an increase in photosynthetically active radiation，the quantum yield of chloroplast photosystem Ⅱ（PSⅡ） photochemistry（Φ₂）exhibited a decreasing trend，while the quantum yield of regulated energy dissipation（Φ_NPQ）showed an increasing trend. The quantum yield of non-regulated energy dissipation（Φ_NO）remained essentially unchanged across different temperatures and was negatively correlated with Φ_NPQ. Lily plants achieved the maximum Φ? when photosynthetically active radiation was 0. The coefficient of determination （R²） between predicted and actual values of Φ? was 0.95，with a relative root mean square error（r_RMSE）of 3.15%. For the fraction of open PSII reaction centers（qL），the R2 was 0.86 and the r_RMSE was 6.15%. For Φ_NPQ，the R2 was 0.95 and the r_RMSE was 9.58%. With increasing drought stress intensity，Φ_NO and Φ_NPQ increased，while the net photosynthetic rate at saturating light（P_n，max），Φ₂，qL，and the maximum photochemical efficiency of PSII（F_v/F_m）decreased. Furthermore，the carbon assimilation capacity per electron（s）and dark respiration rate（R_d）were also reduced. After 10 days of rewatering，P_n，max and $Φ ?$ of lily plants under a soil water potential of -20 to -15 kPa were essentially consistent with those under -10 to -4 kPa. These findings provide a reference for evaluating the growth of lily under drought stress.

Key words: lily, drought stress, chlorophyll fluorescence parameter, diagnosis, water potential

CLC Number:

S644.1

JIANG Yuanjun, YANG Yiping, FU Manman, WU Chao. Diagnosis of drought stress in lilies based on chlorophyll fluorescence parameters[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(7): 1725-1733.

Figures/Tables 11

Table 1 Definition of common chlorophyll fluorescence parameters

叶绿素荧光参数	定义
F_v/F_m	光系统Ⅱ最大光化学量子效率
Φ₂	光适应下PSⅡ实际最大光化学量子效率
qL	PSⅡ反应中心开放比率
Φ_NO	非调节性能量耗散量子产量 μmol?mol^-1
Φ_NPQ	可调性能量耗散量子产量 μmol?mol^-1

Fig.1 Parameter allocation under different photosynthetically active radiations

Fig.2 Changes in chlorophyll fluorescence parameters at different temperatures

Fig.3 Quantum utilization efficiency under different photosynthetically active radiations（PAR）

Fig.4 Maximum and minimum quantum utilization efficiency curves for growth at appropriate temperatures

Fig.5 Fitting curves for different parameters

Fig.6 Changes in chlorophyll fluorescence parameters under drought stres

Fig.7 Changes in chlorophyll fluorescence parameters after drought relief

Fig.8 Relationship between photosynthetic rate and electron transfer rate

Table 2 Fitting values of various slopes and respiratory effects after rehydration

水势/kPa	斜率（s）	暗呼吸速率
-4～-10	0.185	0.655
-10～-15	0.205	0.643
-15～-20	0.189	0.586
-20～-30	0.175	0.531
-30～-40	0.152	0.465

Fig.9 Fitting curves between various parameters

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