浙江农业科学 ›› 2024, Vol. 65 ›› Issue (5): 1019-1024.DOI: 10.16178/j.issn.0528-9017.20230705

• 瓜果类 • 上一篇    下一篇

丝瓜种质资源耐低温弱光性鉴定评价及筛选

陈小央1(), 柴亚倩2, 董文其2, 许云飞2, 邱文敬2, 孙玉燕2,*()   

  1. 1.浙江省种子管理总站,浙江 杭州 310020
    2.浙江省农业科学院 蔬菜研究所,浙江 杭州 310021
  • 收稿日期:2023-07-07 出版日期:2024-05-11 发布日期:2024-05-23
  • 通讯作者: 孙玉燕(1986—),女,山东曹县人,副研究员,博士,研究方向为瓜类遗传育种及分子生物学,E-mail: syy1111@126.com
  • 作者简介:陈小央(1970—),女,浙江诸暨人,高级农艺师,研究方向为农作物种质资源收集、鉴定评价与保护利用技术研究与管理,E-mail: caroline@163.com
  • 基金资助:
    浙江省农业(蔬菜)新品种选育重大科技专项(2021C02065);浙江省瓜菜种质资源精准鉴定

Identification, evaluation and screening for low temperature and low light tolerance in germplasm resources of sponge gourd

CHEN Xiaoyang1(), CHAI Yaqian2, DONG Wenqi2, XU Yunfei2, QIU Wenjing2, SUN Yuyan2,*()   

  1. 1. Zhejiang Seed Management Station, Hangzhou 310020, Zhejiang
    2. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang
  • Received:2023-07-07 Online:2024-05-11 Published:2024-05-23

摘要:

随着设施农业的不断发展,亟需选育出符合设施栽培的耐低温弱光丝瓜新品种,种质资源的耐低温弱光鉴定评价可为新品种选育奠定重要的材料基础。基于此,该研究利用智能人工气候室模拟低温弱光环境,对192份丝瓜种质资源开展耐低温弱光性鉴定,通过冷害分级、冷害指数、相对电导率等指标开展鉴定评价。结果表明,192份丝瓜种质资源的冷害指数范围为35.00%~92.86%,平均冷害指数为61.98%,变异系数为19.50%;相对电导率范围为10.39%~82.00%,平均相对电导率为49.18%,变异系数为24.80%。结合冷害指数(≤45%)和相对电导率(≤30%),筛选出耐低温弱光的丝瓜种质资源11份。将192份种质资源的冷害指数(y)与相对电导率(x)进行相关性分析,二者呈极显著正相关,并获得线性方程y=0.892x+0.181 1(R2=0.810 1)。研究结果可为丝瓜种质资源耐低温弱光鉴定评价及耐低温弱光新品种选育奠定重要的理论和材料基础。

关键词: 丝瓜, 种质资源, 低温弱光, 冷害指数, 相对电导率

Abstract:

With the continuous development of facility agriculture, there is an urgent need to select and breed new sponge gourd varieties with low temperature and low light tolerance. Identification and evaluation of low temperature and low light tolerance of germplasm resources can lay an important material foundation for new variety selection and breeding. In this study, 192 sponge gourd germplasm resources were evaluated for low temperature and low light tolerance in an intelligent artificial climate chamber with simulating low temperature and low light environment. The cold damage classification, cold injury index and relative conductivity for each germplasm resource were identified and evaluated. The results showed that the cold injury index of 192 sponge gourd germplasm resources ranged from 35.00% to 92.86%, with an average of 61.98% and a coefficient of variation of 19.50%; the relative conductivity ranged from 10.39% to 82.00%, with an average of 49.18% and a coefficient of variation of 24.80%. Combining with the cold injury index (≤45%) and relative conductivity (≤30%), 11 sponge gourd germplasm resources with low temperature and low light tolerance were screened. Correlation analysis of cold injury index (y) and relative conductivity (x) of 192 sponge gourd germplasm resources were carried out, and the two parameters were highly significantly and positively correlated. Additionally, the linear equation y=0.892x+0.181 1 (R2=0.810 1) was obtained. The results of the study provided important theoretical guidance and material basis for low temperature and low light tolerance identification, evaluation, selection and new varieties breeding in sponge gourd.

Key words: sponge gourd, germplasm resources, low-temperature and low-light, cold injury index, relative electric conductivity

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