豚鼠源马链球菌兽疫亚种的分离鉴定与耐药性分析

doi:10.16178/j.issn.0528-9017.20240202

摘要/Abstract

摘要：

为鉴定引起某豚鼠养殖场发生急性死亡的病原,对疑似细菌感染的豚鼠进行临床观察、病理剖检,并进行细菌分离纯化,对分离菌株进行革兰氏染色、16S rDNA序列分析及药敏试验。结果显示,死亡豚鼠多脏器中均能分离到同一种致病菌,该菌具有明显的β溶血环,革兰氏染色呈阳性,成对或短链状排列;该分离菌株与马链球菌兽疫亚种 (MK614224) 16S rDNA同源性高达99.77%。药敏试验结果表明,该分离株对头孢噻呋、多西环素等4种药物敏感;对红霉素、环丙沙星中度敏感;对头孢噻肟、青霉素等15种药物耐药。本试验结果确定了马链球菌兽疫亚种是引起豚鼠急性死亡的病原,该分离株具有较强的耐药性,为后期豚鼠马链球菌兽疫亚种的防治提供了参考依据。

关键词: 豚鼠, 马链球菌兽疫亚种, 分离鉴定, 耐药性

Abstract:

In order to identify the causative agent of sudden death of guinea pigs at a farm, clinical observation, pathological dissection and bacterial isolation were conducted on guinea pigs suspected of bacterial infection. Gram staining, 16S rDNA sequencing and antimicrobial susceptibility test were performed following bacterial isolation. The results showed that the same pathogenic bacteria could be isolated from multiple organs of dead guinea pigs, which had obvious β- hemolytic rings, Gram positive staining, and were arranged in pairs or short chains. The 16S rDNA sequence of the isolated bacterial strain had a high homology of 99.77% with that of Streptococcus equi subsp. zooepidemicus (MK614224). The results of antimicrobial susceptibility test showed that the isolated bacterial strain was sensitive to 4 drugs, including ceftiofur and doxycycline; Moderate sensitivite to erythromycin and ciprofloxacin; Resistant to 15 drugs including cefotaxime and penicillin. The results of this experiment confirmed that Streptococcus equi subsp. zooepidemicus was the pathogen causing sudden death of guinea pigs, and the isolated strain showed strong drug resistance, providing a reference for the prevention and control of Streptococcus equi subsp. zooepidemicus.

Key words: guinea pig, Streptococcus equi subsp. zooepidemicus, isolation and identification, drug resistance

中图分类号:

S851

陈巧丹, 陈怡洁, 程菊芬, 袁秀芳, 余斌, 苏菲, 叶十一, 徐丽华, 张晖, 李军星. 豚鼠源马链球菌兽疫亚种的分离鉴定与耐药性分析[J]. 浙江农业科学, 2025, 66(6): 1496-1500.

CHEN Qiaodan, CHEN Yijie, CHENG Jufen, YUAN Xiufang, YU Bin, SU Fei, YE Shiyi, XU Lihua, ZHANG Hui, LI Junxing. Isolation, identification, and drug resistance analysis of Streptococcus equi subsp. zooepidemicus from guinea pigs[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(6): 1496-1500.

图/表 6

参考文献 13

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引物名称	引物序列(5'-3')
universal primer-F	AGAGTTTGATCCTGGCTCAG
universal primer-R	GCGGCTGCTGGCACG

引物名称	引物序列(5'-3')
universal primer-F	AGAGTTTGATCCTGGCTCAG
universal primer-R	GCGGCTGCTGGCACG

菌株	GenBank登录号	同源性/%
Streptococcus equi subsp. zooepidemicus strain 10m172	MK614224	99.77
Streptococcus equi subsp. zooepidemicus strain ICMP 20920	MG786415	99.77
Streptococcus equi subsp. zooepidemicus isolate 10/2007	FJ601190	99.77
Streptococcus equi subsp. zooepidemicus strain Bd 19/03	EF406015	99.77
Streptococcus equi subsp. zooepidemicus strain Tokyo1291	AB104843	99.77
Streptococcus equi subsp. zooepidemicus strain 06m07n5	MK614256	99.54
Streptococcus equi subsp. zooepidemicus strain 09m98	MK614240	99.54
Streptococcus equi subsp. zooepidemicus strain 10m178	MK614219	99.54

菌株	GenBank登录号	同源性/%
Streptococcus equi subsp. zooepidemicus strain 10m172	MK614224	99.77
Streptococcus equi subsp. zooepidemicus strain ICMP 20920	MG786415	99.77
Streptococcus equi subsp. zooepidemicus isolate 10/2007	FJ601190	99.77
Streptococcus equi subsp. zooepidemicus strain Bd 19/03	EF406015	99.77
Streptococcus equi subsp. zooepidemicus strain Tokyo1291	AB104843	99.77
Streptococcus equi subsp. zooepidemicus strain 06m07n5	MK614256	99.54
Streptococcus equi subsp. zooepidemicus strain 09m98	MK614240	99.54
Streptococcus equi subsp. zooepidemicus strain 10m178	MK614219	99.54

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