涝渍胁迫下铁线莲美登丽的生理生化响应

doi:10.16178/j.issn.0528-9017.20260260

摘要/Abstract

摘要：

为分析铁线莲对涝渍胁迫的耐受特征，本研究以铁线莲美登丽为试材，设置对照、半淹水与全淹水处理，系统分析了涝渍胁迫下铁线莲的生长表型、光合特性、叶绿素含量、丙二醛（MDA）含量、抗氧化酶活性及1-氨基环丙烷-1-羧酸（ACC）和ATP含量的变化规律。结果表明，全淹水胁迫抑制美登丽生长，植株出现明显萎蔫与叶片脱落，与对照相比，净光合速率显著（p<0.05）降低84.55%，总叶绿素含量显著降低28.15%，MDA含量显著提高53.22%，ACC含量显著降低69.92%，ATP含量显著降低84.65%，过氧化物酶与过氧化氢酶等抗氧化酶活性显著提高。半淹水胁迫下，ACC含量达到最大值，表明涝渍胁迫下铁线莲可通过ACC积累和抗氧化酶系统的差异化调节启动一定的适应性响应，但持续严重淹水导致能量代谢急剧衰退、膜脂过氧化加剧和光合系统严重受损，超出植株的生理耐受阈值，对生长造成显著抑制。该研究结果为铁线莲的抗逆品种选育与栽培管理提供了理论依据。

关键词: 铁线莲, 涝渍胁迫, 生理响应, 抗氧化酶, 光合特性

Abstract:

To analyze the tolerance characteristics of Clematis to waterlogging stress，this study used Clematis Midori as the experimental material and set up three treatments：control，half-waterlogging，and full-waterlogging. The growth phenotype，photosynthetic characteristics，chlorophyll content，malondialdehyde（MDA）content，antioxidant enzyme activity，and 1-aminocyclopropane-1-carboxylic acid（ACC）and ATP contents of Clematis under waterlogging stress were systematically analyzed. The results showed that full-waterlogging stress inhibited the growth of Clematis Midori，with obvious wilting and leaf abscission，compared with the control，the net photosynthetic rate significantly（p<0.05）decreased by 84.55%，total chlorophyll content significantly decreased by 28.15%，MDA content significantly increased by 53.22%，ACC content significantly decreased by 69.92%，and ATP content significantly decreased by 84.65%. Meanwhile，the activities of antioxidant enzymes such as peroxidase and catalase were significantly increased. ACC content reached the highest value under half-waterlogging stress. These results indicate that Clematis can initiate certain adaptive responses through ACC accumulation and the differential regulation of the antioxidant enzyme system under waterlogging stress. However，continuous severe waterlogging leads to a sharp decline in energy metabolism，aggravated membrane lipid peroxidation，and severe damage to the photosynthetic system，exceeding the physiological tolerance threshold of the plant and significantly inhibiting growth. These findings provide a theoretical basis for the breeding of stress-resistant varieties and cultivation management of Clematis.

Key words: Clematis, waterlogging stress, physiological response, antioxidant enzyme, photosynthetic characteristic

中图分类号:

S682.19

张卫国, 章海英, 黄爱云, 林琳, 胡亚平, 胡青荻, 张旭乐, 陈义增. 涝渍胁迫下铁线莲美登丽的生理生化响应[J]. 浙江农业科学, 2026, 67(9): 2211-2217.

ZHANG Weiguo, ZHANG Haiying, HUANG Aiyun, LIN Lin, HU Yaping, HU Qingdi, ZHANG Xule, CHEN Yizeng. Physiological and biochemical responses of Clematis Midori under waterlogging stress[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(9): 2211-2217.

图/表 6

图1 半淹水与全淹水处理下铁线莲美登丽的生长

Fig.1 Growth of Clematis Midori under half-waterlogging and full-waterlogging treatments

图2 半淹水与全淹水处理下铁线莲美登丽的丙二醛（MDA）及H2O2含量柱上无相同小写字母表示不同处理间差异显著（p<0.05），图3～6同。

Fig.2 Malondialdehyde （MDA） and H2O2 contents of Clematis Midori under half-waterlogging and full-waterlogging treatments

图3 半淹水与全淹水处理下铁线莲美登丽的光合参数Pn，净光合速率；Tr，蒸腾速率；Gs，气孔导度；Ci，胞间CO2浓度。

Fig. 3 Photosynthetic indicators of Clematis Midori under half-waterlogging and full-waterlogging treatments

图4 半淹水与全淹水处理下铁线莲美登丽的叶绿素含量

Fig.4 Chlorophyll contents of Clematis Midori under half-waterlogging and full-waterlogging treatments

图5 半淹水与全淹水处理下铁线莲美登丽的抗氧化酶活性SOD，超氧化物歧化酶；POD，过氧化物酶；CAT，过氧化氢酶；APX，抗坏血酸过氧化物酶。

Fig.5 Antioxidant enzyme activities of Clematis Midori under half-waterlogging and full-waterlogging treatments

图6 半淹水与全淹水处理下铁线莲美登丽1-氨基环丙烷-1-羧酸（ACC）含量及ATP含量

Fig.6 1-Aminocyclopropane-1-carboxylic acid （ACC） and ATP contents of Clematis Midori under half-waterlogging and full-waterlogging treatments

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