基于网络药理学的铁皮石斛免疫调节作用机制

doi:10.16178/j.issn.0528-9017.20212931

摘要/Abstract

摘要：

研究表明,铁皮石斛在增强人体免疫力方面具有良好作用。为了明确铁皮石斛潜在的免疫活性成分及其作用机制,本研究从PubChem、SciFinder数据库中收集铁皮石斛小分子化合物及其化学结构相关的研究,然后通过Swiss ADME筛选优效目标化合物。通过Swiss Target Prediction预测优效化合物的靶点集,并通过Genecards数据库获取免疫调节相关靶点集,两者取交集得到铁皮石斛免疫调节相关靶点集,进一步通过Cytoscape软件建立化合物-靶点网络图,将交集靶点导入STRING构建蛋白互作网络(PPI),筛选出核心靶点。通过R的Cluster Profiler包对核心靶点进行功能富集分析,包括基因本体分析(GO)及通路富集分析(KEGG),最终建立化合物-靶点-生物功能网络。最终,共筛选出优效化合物124个,对应靶点421个;与免疫调节相关化合物58个,对应靶点60个;其中黄酮类化合物对应的免疫靶点较多,可能为主要的免疫活性成分。得到9个免疫调节相关核心靶点,GO分析发现这些核心靶点共参与了2 059个条目,KEGG分析获得153个代谢通路。本研究通过化合物-靶点-生物功能网络展示了铁皮石斛多成分、多靶点、多通路的作用特点,为后续作用机制的深入研究和验证提供了可行方向。

关键词: 铁皮石斛, 网络药理学, 靶点, 黄酮, 作用机制

中图分类号:

S567
Q1

李振宇, 赵建霞, 张国亮, 王晓彤, 王瑛, 徐靖, 李明焱, 李振皓. 基于网络药理学的铁皮石斛免疫调节作用机制[J]. 浙江农业科学, 2022, 63(8): 1855-1860.

图/表 4

图1 化合物-铁皮石斛免疫调节靶点网络内圈节点为化合物;外圈节点为免疫调节靶点。

图2 铁皮石斛免疫调节相关靶点PPI网络

图3 核心靶点GO、KEGG富集分析结果 A—细胞组分注释结果;B—生物过程注释结果;C—分子水平注释结果;D—核心靶点KEGG富集分析结果。

图4 化合物-核心靶点-生物功能网络中间竖排黄色节点为核心靶点,左侧圈状排列节点为铁皮石斛化合物,右侧圈状排列节点为核心靶点富集到的生物功能。

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