浙江农业科学 ›› 2025, Vol. 66 ›› Issue (6): 1429-1432.DOI: 10.16178/j.issn.0528-9017.20240266

• 植保技术 • 上一篇    下一篇

甘蔗叶斑病病原鉴定、生物学特性研究及防治药剂筛选

张育青1(), 武军2,*(), 方丽2, 金雯轩1, 王汉荣2,*()   

  1. 1.浙江省农业技术推广中心,浙江 杭州 310021
    2.浙江省农业科学院 植物保护与微生物研究所,浙江 杭州 310021
  • 收稿日期:2024-04-01 出版日期:2025-06-11 发布日期:2025-06-23
  • 通讯作者: 王汉荣(1966—),男,浙江杭州人,研究员,主要从事经济作物病害诊断及绿色防控技术研发,E-mail:ZJNKYJJBH@hotmail.com;武军(1986—),男,山西临汾人,助理研究员,主要从事经济作物病害诊断及绿色防控技术研究,E-mail:windows5009@sina.com
  • 作者简介:张育青(1972—),女,浙江台州人,高级农艺师,主要从事农业技术推广工作,E-mail:zhang699119@163.com
  • 基金资助:
    浙江省“三农九方”科技协作计划(2022SNFJ076)

Pathogen identification, biological characteristics and fungicides screening of leaf spot disease of Saccharum officinarum

ZHANG Yuqing1(), WU Jun2,*(), FANG Li2, JIN Wenxuan1, WANG Hanrong2,*()   

  1. 1. Zhejiang Agricultural Technical Extension Center, Hangzhou 310021, Zhejiang
    2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang
  • Received:2024-04-01 Online:2025-06-11 Published:2025-06-23

摘要:

从浙江省台州市温岭市采集发病的甘蔗叶片和茎段,通过病原菌分离、科赫氏法则验证和分子生物学测定,结果显示,甘蔗叶斑病病原菌为新月弯孢(Curvularia lunata)。探究碳源、氮源、温度、光照和pH值对病原菌生长的影响,结果显示,菌株GZWB21在6种碳源上生长良好,最适碳源为蔗糖,在6种氮源上生长良好,最适氮源为酵母粉,最适生长温度为35 ℃,最适pH值为8。43%氟菌·肟菌酯悬浮剂、450 g·L-1咪鲜胺水乳剂和50%啶酰菌胺水分散粒剂对GZWB21具有较强的抑制作用,有效抑制中浓度(EC50)分别为0.003 8、0.002 7和0.124 0 mg·L-1。本研究可为甘蔗叶斑病的防治提供参考。

关键词: 甘蔗, 叶斑病, 病原, 生物学特性, 防治药剂

Abstract:

The diseased leaves and stems of Saccharum officinarum were collected from Wenling County, Taizhou City, Zhejiang Province. The results showed that the pathogens were identified as Curvularia lunata based on pathogen isolation, Koch postulates and molecular identification. The effect of carbon source, nitrogen source, temperature, light and pH value on growth of GZWB21 were determined, these results suggested that strain GZWB21 grew well on six carbon sources, the optimal carbon source and nitrogen source of GZWB21 were sucrose and yeast powder, respectively. The optimal temperature was 35℃, the optimal pH value was 8. Besides, 43% fluoromystrobin suspension, 450 g·L-1 prochloraz aqueous emulsion and 50% acetimidine aqueous dispersible granules had a strong inhibitory effect on GZWB21, with EC50 of 0.003 8、0.002 7 and 0.124 0 mg·L-1. This study can provide a reference for the prevention and control of leaf spot disease of Saccharum officinarum.

Key words: Saccharum officinarum, leaf spot, pathogen, biological characteristics, fungicides

中图分类号: