浙江省稻瘟病菌无毒基因分析

doi:10.16178/j.issn.0528-9017.20240422

摘要/Abstract

摘要：

针对集自浙江省各稻区的170个稻瘟病菌单孢菌株,选取7个稻瘟病菌无毒基因的特异性引物对其进行PCR检测,结果显示,有6个无毒基因在供试菌株中被检出,不同地区各无毒基因的检出频率不同,检出率最高的无毒基因是Avr-pik,达到98.8%,Avr1-co39未检出;来源于同一地区的菌株,其寄主品种不同,其无毒基因组成也不同;来源于不同地区的同一寄主品种上的菌株,其无毒基因组成也不同。

关键词: 稻瘟病菌, 无毒基因, 出现频率, 浙江省

Abstract:

PCR detection was performed on 170 Magnaporthe oryza strains collected from various rice-growing regions in Zhejiang Province using specific primers for seven avirulence genes. The results revealed that six avirulence genes were detected in the tested strains, with varying occurrence frequencies across different regions. The most prevalent avirulence gene was Avr-pik, whose detection rate was 98.8%, while Avr1-co39 was undetected. Strains from the same region but infecting different host cultivars displayed distinct avirulence gene profiles. Similarly, isolates from different regions infecting the same host cultivar also exhibited differences in avirulence gene composition.

Key words: Magnaporthe oryzae, avirulence gene, occurrence frequency, Zhejiang Province

中图分类号:

S435.111.41

毛雪琴, 邱海萍, 柴荣耀, 郝中娜, 张震, 王教瑜, 孙国仓, 王艳丽. 浙江省稻瘟病菌无毒基因分析[J]. 浙江农业科学, 2025, 66(10): 2426-2431.

MAO Xueqin, QIU Haiping, CHAI Rongyao, HAO Zhongna, ZHANG Zhen, WANG Jiaoyu, SUN Guocang, WANG Yanli. Analysis of avirulence genes in Magnaporthe oryzae in Zhejiang Province[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2426-2431.

图/表 5

参考文献 18

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寄主水稻品种	各地区菌株数量						合计
寄主水稻品种	宁波	嘉兴	绍兴	湖州	杭州	温州	合计
宁81	9	0	0	0	0	0	9
宁84	25	0	0	0	0	0	21
宁88	4	0	0	0	0	0	4
宁1268	3	0	0	0	0	0	3
宁1355	2	0	0	0	0	0	2
甬优12	10	0	0	0	0	0	10
甬优15	6	0	0	0	0	0	6
甬优360	4	0	0	0	0	0	4
甬优538	1	0	0	0	0	0	1
甬优720	2	0	0	0	0	0	2
甬优1140	2	0	0	0	0	0	2
甬优1640	3	0	0	0	0	0	3
甬优2640	3	0	0	0	0	0	3
甬优2050	3	0	0	0	0	0	3
嘉禾218	1	1	0	0	0	0	2
嘉58	3	0	0	0	3	0	6
秀水134	0	4	0	0	0	0	4
秀水321	3	11	8	1	0	0	23
绍粳18	6	4	0	0	2	0	12
浙粳88	6	14	0	3	0	0	23
绍糯	3	0	0	0	0	0	3
籼稻	0	0	1	0	0	3	4
粳稻3	0	0	3	0	0	0	3
粳稻2	0	0	1	0	0	0	1
粳稻1	0	0	1	0	0	0	1
闵优香粳	0	1	0	0	0	0	1
天两优616	0	0	0	2	0	0	2
武运粳23号	0	0	0	3	0	0	3
武运粳24号	0	0	0	2	0	0	2
丙13105	3	0	0	0	0	0	3
合计	102	35	14	11	5	3	170

寄主水稻品种	各地区菌株数量						合计
寄主水稻品种	宁波	嘉兴	绍兴	湖州	杭州	温州	合计
宁81	9	0	0	0	0	0	9
宁84	25	0	0	0	0	0	21
宁88	4	0	0	0	0	0	4
宁1268	3	0	0	0	0	0	3
宁1355	2	0	0	0	0	0	2
甬优12	10	0	0	0	0	0	10
甬优15	6	0	0	0	0	0	6
甬优360	4	0	0	0	0	0	4
甬优538	1	0	0	0	0	0	1
甬优720	2	0	0	0	0	0	2
甬优1140	2	0	0	0	0	0	2
甬优1640	3	0	0	0	0	0	3
甬优2640	3	0	0	0	0	0	3
甬优2050	3	0	0	0	0	0	3
嘉禾218	1	1	0	0	0	0	2
嘉58	3	0	0	0	3	0	6
秀水134	0	4	0	0	0	0	4
秀水321	3	11	8	1	0	0	23
绍粳18	6	4	0	0	2	0	12
浙粳88	6	14	0	3	0	0	23
绍糯	3	0	0	0	0	0	3
籼稻	0	0	1	0	0	3	4
粳稻3	0	0	3	0	0	0	3
粳稻2	0	0	1	0	0	0	1
粳稻1	0	0	1	0	0	0	1
闵优香粳	0	1	0	0	0	0	1
天两优616	0	0	0	2	0	0	2
武运粳23号	0	0	0	3	0	0	3
武运粳24号	0	0	0	2	0	0	2
丙13105	3	0	0	0	0	0	3
合计	102	35	14	11	5	3	170

无毒基因	正向引物序列(5'-3')	反向引物序列(5'-3')	片段长度/bp	参考文献
Avr-pita	CGACCCGTTTCCGCCTTTATT	TCCCTCCATTCCAACACTAACG	1 076	[11]
ACE1	CCCAGAGTTGGCGATGATGC	ATGTGGCGGTGACAGAGGAC	661	[11]
Avr-pia	TCTCGGGAAGCTGATTGAGT	CTAACGGCCGGCTAACAAAC	265	[12]
Avr-pit	GAATCGCAGCACTTCCCACT	ATAACTGTCCGCCGCTCGT	287	[12]
Avr1-co39	AATTGCATAATCGCTGCGAT	GTCAAGCTCAGAACTTTGTT	918	[13]
Avr-pik	ACTTTGGGAACTGTCGCTGTC	AGCTGTAACAGGTTCCAGCATC	184	[14]
Avr-pizt	AAACCAGGGCAGCCAAAGA	ATTCCCAATCGAGCCAACG	153	[14]

无毒基因	正向引物序列(5'-3')	反向引物序列(5'-3')	片段长度/bp	参考文献
Avr-pita	CGACCCGTTTCCGCCTTTATT	TCCCTCCATTCCAACACTAACG	1 076	[11]
ACE1	CCCAGAGTTGGCGATGATGC	ATGTGGCGGTGACAGAGGAC	661	[11]
Avr-pia	TCTCGGGAAGCTGATTGAGT	CTAACGGCCGGCTAACAAAC	265	[12]
Avr-pit	GAATCGCAGCACTTCCCACT	ATAACTGTCCGCCGCTCGT	287	[12]
Avr1-co39	AATTGCATAATCGCTGCGAT	GTCAAGCTCAGAACTTTGTT	918	[13]
Avr-pik	ACTTTGGGAACTGTCGCTGTC	AGCTGTAACAGGTTCCAGCATC	184	[14]
Avr-pizt	AAACCAGGGCAGCCAAAGA	ATTCCCAATCGAGCCAACG	153	[14]

菌株来源	菌株总数	无毒基因扩出频率/%
菌株来源	菌株总数	Avr-pik	Avr-pizt	ACE1	Avr-pita	Avr-pia	Avr-pit	Avr1-co39
宁波	102	98.0	99.0	93.1	79.4	47.1	2.0	0
嘉兴	35	100.0	94.3	85.7	85.7	54.3	0	0
绍兴	14	100.0	100.0	92.9	78.6	100.0	7.1	0
湖州	11	100.0	100.0	100.0	72.7	81.8	9.1	0
杭州	5	100.0	100.0	100.0	100.0	60.0	20.0	0
温州	3	100.0	100.0	100.0	66.7	100.0	0	0
合计	170	98.8	98.2	92.4	80.6	56.5	2.9	0