Simultaneous determination of 16 elements in tea by nitric acid extraction inductively coupled plasma mass spectrometry

doi:10.16178/j.issn.0528-9017.20230821

Abstract

Abstract:

Establish the optimal determination method for 16 elements including Pb, Cd, As, Cu, Zn, Cr, Co, Mo in tea by nitric acid extraction microwave digestion inductively coupled plasma mass spectrometry (ICP-MS). After microwave digestion of the tea standard substance GBW10052a (GSB-30a), the standard curve method combined with online addition of internal standard solution was used to simultaneously determine the content of 16 elements in collision mode using external standard method and standard addition method. The influence of the tea matrix on the quantitative analysis of the measured elements was studied. The results showed that this method can simultaneously determine multiple elements, with a wide linear range. The correlation coefficient R² of the measured elements is 0.998-1.000. The recovery rates of the three concentration levels added are 88.56%-120.24%, with a relative standard deviation of 0.72%-2.67%. The detection limit of the method is 0.001-0.200 mg·kg^-1. The standard addition method can effectively eliminate the interference of the matrix and can be used for the detection of heavy metal elements in daily tea samples.

Key words: tea, nitric acid, inductively coupled plasma mass spectrometry, microwave, digestion

CLC Number:

S571.1

NING Jianmei, XU Xiaoan, TANG Shiqin, PAN Yingjie. Simultaneous determination of 16 elements in tea by nitric acid extraction inductively coupled plasma mass spectrometry[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(6): 1457-1462.

Figures/Tables 6

References 9

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步骤	升温时间/ min	功率/ W	恒温温度/ ℃	恒温时间/ min
1	5	800	120	2
2	5	800	150	10
3	5	800	190	15

步骤	升温时间/ min	功率/ W	恒温温度/ ℃	恒温时间/ min
1	5	800	120	2
2	5	800	150	10
3	5	800	190	15

序号	元素	标准系列质量浓度/(μg·L^-1)
序号	元素	STD0	STD1	STD2	STD3	STD4	STD5	STD6	STD7
1	Cd	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
2	As	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
3	Sb	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
4	TI	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
5	V	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
6	Cr	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
7	Co	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
8	Mo	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
9	B	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
10	Ba	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
11	Sr	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
12	Zn	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
13	Pb	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
14	Ni	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
15	Ti	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
16	Cu	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0

序号	元素	标准系列质量浓度/(μg·L^-1)
序号	元素	STD0	STD1	STD2	STD3	STD4	STD5	STD6	STD7
1	Cd	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
2	As	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
3	Sb	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
4	TI	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
5	V	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
6	Cr	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
7	Co	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
8	Mo	0.0	0.2	0.5	1.0	2.0	5.0	10.0	20.0
9	B	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
10	Ba	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
11	Sr	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
12	Zn	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
13	Pb	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
14	Ni	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
15	Ti	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0
16	Cu	0.0	2.0	5.0	10.0	20.0	50.0	100.0	200.0

序号	元素	线性回归方程	相关系数(R²)	线性范围/(μg·L^-1)	检出限/(mg·kg^-1)
1	¹¹B	Y=2.218x+10.25	0.999	2~200	0.100
2	¹³⁷Ba	Y=98.73x+22.07	1.000	2~200	0.020
3	⁸⁸Sr	Y=88.23x+122.8	1.000	2~200	0.200
4	⁶⁶Zn	Y=23.02x+144.7	0.999	2~200	0.050
5	²⁰⁸Pb	Y=6 702.59x+2 077.52	1.000	2~200	0.020
6	²⁰⁶Pb	Y=1 629.99x+500.34	1.000	2~200	0.020
7	²⁰⁷Pb	Y=1 423.81x+438.57	1.000	2~200	0.020
8	⁶¹Ni	Y=8.611x+3.166	0.999	2~200	0.200
9	⁴⁸Ti	Y=191.55x+123.15	1.000	2~200	0.020
10	⁶³Cu	Y=1 303.58x+920.99	1.000	2~200	0.050
11	¹¹¹Cd	Y=109.4x+8.122	0.998	0.2~20	0.002
12	¹¹⁴Cd	Y=264.3x+2.904	0.999	0.2~20	0.002
13	⁷⁵As	Y=72.77x+2.648	0.999	0.2~20	0.002
14	¹²¹Sb	Y=163.3x+0.676	0.999	0.2~20	0.001
15	²⁰⁵TI	Y=5 154.80x-144.73	1.000	0.2~20	0.001
16	⁵¹V	Y=569.66x+43.25	1.000	0.2~20	0.002
17	⁵²Cr	Y=725.53x+73.34	1.000	0.2~20	0.050
18	⁵³Cr	Y=89.20x+5.27	1.000	0.2~20	0.050
19	⁵⁹Co	Y=1 617.15x+108.45	1.000	0.2~20	0.002
20	⁹⁵Mo	Y=605.01x+6.29	1.000	0.2~20	0.010