Abstract:

To investigate the effects of different hormone ratios on the proliferation and rooting induction of tissue culture seedlings of Corydalis ophiocarpa Hook. f. et Thoms. MS was used as the basic medium, IBA(0.2, 0.5, 1.0 mg·L^-1), IAA(0.1, 0.2, 0.5 mg·L^-1), and 6-BA(0.3, 0.6, 1.0 mg·L^-1) were tested by three-level and three-factor orthogonal test to screen the best proliferation medium. NAA(0.5, 1.0, 2.0 mg·L^-1) and IBA(0.5, 1.0, 2.0 mg·L^-1) were used to select the best medium for inducing rooting. The results showed that the culture medium MS+0.2 mg·L^-1 IBA+0.1 mg·L^-1 IAA+0.3 mg·L^-1 6-BA had the best proliferation effect, with a multiplication rate of more than 8 times, and was suitable for inducing the differentiation of adventitious buds in Corydalis ophiocarpa Hook. f. et Thoms., effectively increasing the number of seedlings. MS+0.5 mg·L^-1 NAA(pH value 5.8) was identified as the optimal culture medium for the rooting of main roots in Corydalis ophiocarpa Hook. f. et Thoms., while MS+1.0 mg·L^-1 IBA(pH value 5.8) was the optimal culture medium for the rooting of fibrous roots. This study has determined the best culture medium formulas for the proliferation and rooting of Corydalis ophiocarpa Hook. f. et Thoms., significantly improving the efficiency and quality of rooting, providing an important basis for efficient propagation of Corydalis ophiocarpa Hook. f. et Thoms..

Key words: Corydalis ophiocarpa Hook. f. et Thoms, tissue culture, proliferation, rooting