
Journal of Zhejiang Agricultural Sciences ›› 2026, Vol. 67 ›› Issue (2): 376-381.DOI: 10.16178/j.issn.0528-9017.20240810
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LI Mingjiang1(
), SHENG Yuqi2, ZHANG Jiaqiang1(
)
Received:2024-10-21
Online:2026-02-28
Published:2026-03-07
CLC Number:
LI Mingjiang, SHENG Yuqi, ZHANG Jiaqiang. Effects of different hormones and substrates on cuttage rooting of Tetrastigma hemsleyanum[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(2): 376-381.
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URL: http://www.zjnykx.cn/EN/10.16178/j.issn.0528-9017.20240810
| 试验水平 | 试验因素 | |||
|---|---|---|---|---|
| 激素种类(A) | 激素浓度(B)/(mg·L-1) | 蘸根时间(C)/min | 基质类型(D) | |
| 1 | IBA | 100 | 5 | 纯河沙 |
| 2 | NAA | 200 | 10 | 纯泥炭 |
| 3 | IAA | 300 | 15 | 混合基质(河沙、泥炭、珍珠岩体积比1∶1∶1) |
Table 1 Orthogonal experimental design
| 试验水平 | 试验因素 | |||
|---|---|---|---|---|
| 激素种类(A) | 激素浓度(B)/(mg·L-1) | 蘸根时间(C)/min | 基质类型(D) | |
| 1 | IBA | 100 | 5 | 纯河沙 |
| 2 | NAA | 200 | 10 | 纯泥炭 |
| 3 | IAA | 300 | 15 | 混合基质(河沙、泥炭、珍珠岩体积比1∶1∶1) |
| 处理编号 | 试验设计方案 | 试验结果 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | 平均根数 | 平均根长/cm | 根鲜重/g | 生根效果指数 | 生根率/% | |
| 1 | 1 | 1 | 1 | 1 | 10.02 f | 9.8 b | 0.21 c | 2.54 b | 68.96 bc |
| 2 | 1 | 2 | 2 | 2 | 19.16 c | 11.7 ab | 0.42 abc | 1.61 b | 56.33 de |
| 3 | 1 | 3 | 3 | 3 | 18.23 cd | 11.6 ab | 0.20 c | 1.70 b | 51.76 e |
| 4 | 2 | 1 | 2 | 3 | 24.05 ab | 12.7 ab | 0.47 abc | 7.14 a | 74.76 b |
| 5 | 2 | 2 | 3 | 1 | 19.13 c | 9.8 b | 0.30 bc | 2.86 b | 63.09 cd |
| 6 | 2 | 3 | 1 | 2 | 23.21 b | 11.2 b | 0.58 ab | 1.59 b | 53.09 e |
| 7 | 3 | 1 | 3 | 2 | 15.20 de | 10.3 b | 0.37 abc | 3.74 ab | 58.09 de |
| 8 | 3 | 2 | 1 | 3 | 14.18 e | 11.3 b | 0.53 ab | 6.66 a | 71.43 bc |
| 9 | 3 | 3 | 2 | 1 | 27.15 a | 15.1 a | 0.62 a | 3.99 ab | 85.31 a |
Table 2 Cuttage rooting indicators of Tetrastigma hemsleyanum under different treatments
| 处理编号 | 试验设计方案 | 试验结果 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | 平均根数 | 平均根长/cm | 根鲜重/g | 生根效果指数 | 生根率/% | |
| 1 | 1 | 1 | 1 | 1 | 10.02 f | 9.8 b | 0.21 c | 2.54 b | 68.96 bc |
| 2 | 1 | 2 | 2 | 2 | 19.16 c | 11.7 ab | 0.42 abc | 1.61 b | 56.33 de |
| 3 | 1 | 3 | 3 | 3 | 18.23 cd | 11.6 ab | 0.20 c | 1.70 b | 51.76 e |
| 4 | 2 | 1 | 2 | 3 | 24.05 ab | 12.7 ab | 0.47 abc | 7.14 a | 74.76 b |
| 5 | 2 | 2 | 3 | 1 | 19.13 c | 9.8 b | 0.30 bc | 2.86 b | 63.09 cd |
| 6 | 2 | 3 | 1 | 2 | 23.21 b | 11.2 b | 0.58 ab | 1.59 b | 53.09 e |
| 7 | 3 | 1 | 3 | 2 | 15.20 de | 10.3 b | 0.37 abc | 3.74 ab | 58.09 de |
| 8 | 3 | 2 | 1 | 3 | 14.18 e | 11.3 b | 0.53 ab | 6.66 a | 71.43 bc |
| 9 | 3 | 3 | 2 | 1 | 27.15 a | 15.1 a | 0.62 a | 3.99 ab | 85.31 a |
| 试验因素 | 平均根数 | 平均根长/cm | 根鲜重/g | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| k1 | k 2 | k 3 | R | k1 | k 2 | k 3 | R | k1 | k 2 | k 3 | R | ||
| 激素种类(A) | 15.67 | 22.00 | 18.67 | 6.33 | 11.03 | 11.23 | 12.23 | 1.20 | 0.28 | 0.45 | 0.51 | 0.23 | |
| 激素浓度(B) | 16.33 | 17.33 | 22.67 | 6.33 | 10.93 | 10.93 | 12.63 | 1.70 | 0.35 | 0.42 | 0.47 | 0.12 | |
| 蘸根时间(C) | 15.67 | 23.33 | 17.33 | 7.67 | 10.77 | 13.17 | 10.57 | 2.60 | 0.44 | 0.50 | 0.29 | 0.21 | |
| 基质类型(D) | 18.67 | 19.00 | 18.67 | 0.33 | 11.57 | 11.07 | 11.87 | 0.80 | 0.38 | 0.46 | 0.40 | 0.08 | |
| 试验因素 | 生根效果指数 | 生根率/% | |||||||||||
| k1 | k2 | k3 | R | k1 | k 2 | k 3 | R | ||||||
| 激素种类(A) | 1.95 | 3.86 | 4.80 | 2.85 | 59.02 | 63.65 | 71.61 | 12.59 | |||||
| 激素浓度(B) | 4.47 | 3.71 | 2.43 | 2.05 | 67.27 | 63.62 | 63.39 | 3.88 | |||||
| 蘸根时间(C) | 3.60 | 4.25 | 2.77 | 1.48 | 64.49 | 72.13 | 57.65 | 14.49 | |||||
| 基质类型(D) | 3.13 | 2.31 | 5.17 | 2.85 | 72.45 | 55.84 | 65.98 | 16.62 | |||||
Table 3 Orthogonal analysis of cuttage rooting indicators of Tetrastigma hemsleyanum
| 试验因素 | 平均根数 | 平均根长/cm | 根鲜重/g | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| k1 | k 2 | k 3 | R | k1 | k 2 | k 3 | R | k1 | k 2 | k 3 | R | ||
| 激素种类(A) | 15.67 | 22.00 | 18.67 | 6.33 | 11.03 | 11.23 | 12.23 | 1.20 | 0.28 | 0.45 | 0.51 | 0.23 | |
| 激素浓度(B) | 16.33 | 17.33 | 22.67 | 6.33 | 10.93 | 10.93 | 12.63 | 1.70 | 0.35 | 0.42 | 0.47 | 0.12 | |
| 蘸根时间(C) | 15.67 | 23.33 | 17.33 | 7.67 | 10.77 | 13.17 | 10.57 | 2.60 | 0.44 | 0.50 | 0.29 | 0.21 | |
| 基质类型(D) | 18.67 | 19.00 | 18.67 | 0.33 | 11.57 | 11.07 | 11.87 | 0.80 | 0.38 | 0.46 | 0.40 | 0.08 | |
| 试验因素 | 生根效果指数 | 生根率/% | |||||||||||
| k1 | k2 | k3 | R | k1 | k 2 | k 3 | R | ||||||
| 激素种类(A) | 1.95 | 3.86 | 4.80 | 2.85 | 59.02 | 63.65 | 71.61 | 12.59 | |||||
| 激素浓度(B) | 4.47 | 3.71 | 2.43 | 2.05 | 67.27 | 63.62 | 63.39 | 3.88 | |||||
| 蘸根时间(C) | 3.60 | 4.25 | 2.77 | 1.48 | 64.49 | 72.13 | 57.65 | 14.49 | |||||
| 基质类型(D) | 3.13 | 2.31 | 5.17 | 2.85 | 72.45 | 55.84 | 65.98 | 16.62 | |||||
| 相关系数 | 平均根长 | 根鲜重 | 生根效果指数 | 生根率 |
|---|---|---|---|---|
| 平均根数 | 0.775 2** | 0.645 5* | 0.091 8 | 0.284 5 |
| 平均根长 | 0.6451* | 0.286 4 | 0.599 5 | |
| 根鲜重 | 0.382 3 | 0.434 0 | ||
| 生根效果指数 | 0.660 5* |
Table 4 Correlation analysis of cuttage rooting indicators of Tetrastigma hemsleyanum under different treatments
| 相关系数 | 平均根长 | 根鲜重 | 生根效果指数 | 生根率 |
|---|---|---|---|---|
| 平均根数 | 0.775 2** | 0.645 5* | 0.091 8 | 0.284 5 |
| 平均根长 | 0.6451* | 0.286 4 | 0.599 5 | |
| 根鲜重 | 0.382 3 | 0.434 0 | ||
| 生根效果指数 | 0.660 5* |
| 处理编号 | 关联矩阵 | 灰色关联系数 | 排序 |
|---|---|---|---|
| 1 | A1B1C1D1 | 0.421 4 | 8 |
| 2 | A1B2C2D2 | 0.601 5 | 4 |
| 3 | A1B3C3D3 | 0.484 2 | 7 |
| 4 | A2B1C2D3 | 0.790 1 | 1 |
| 5 | A2B2C3D1 | 0.618 8 | 3 |
| 6 | A2B3C1D2 | 0.404 4 | 9 |
| 7 | A3B1C3D2 | 0.553 0 | 5 |
| 8 | A3B2C1D3 | 0.524 5 | 6 |
| 9 | A3B3C2D1 | 0.666 4 | 2 |
Table 5 Gray association analysis results
| 处理编号 | 关联矩阵 | 灰色关联系数 | 排序 |
|---|---|---|---|
| 1 | A1B1C1D1 | 0.421 4 | 8 |
| 2 | A1B2C2D2 | 0.601 5 | 4 |
| 3 | A1B3C3D3 | 0.484 2 | 7 |
| 4 | A2B1C2D3 | 0.790 1 | 1 |
| 5 | A2B2C3D1 | 0.618 8 | 3 |
| 6 | A2B3C1D2 | 0.404 4 | 9 |
| 7 | A3B1C3D2 | 0.553 0 | 5 |
| 8 | A3B2C1D3 | 0.524 5 | 6 |
| 9 | A3B3C2D1 | 0.666 4 | 2 |
| [1] | HU W Y, ZHENG Y J, XIA P G,et al. The research progresses and future prospects of Tetrastigma hemsleyanum Diels et Gilg:a valuable Chinese herbal medicine[J]. Journal of Ethnopharmacology,2021,271:113836. |
| [2] | 熊利权,杨德军,邱琼,等. 不同基质和激素处理对针叶樱桃嫩枝扦插生根的影响[J]. 经济林研究,2021,39(3):142-149. |
| XIONG L Q, YANG D J, QIU Q,et al. Effects of different matrix and hormones on rooting of tender cuttings of Malpighia glabra L[J]. Non-wood Forest Research,2021,39(3):142-149. | |
| [3] | 玉屏,姚雾清,莫家伟,等. 植物激素对三叶青扦插生根的影响[J]. 现代园艺,2018(18):18. |
| YU P, YAO W Q, MO J W,et al. Effects of plant hormones on rooting of clover cutting[J]. Xiandai Horticulture,2018(18):18. | |
| [4] | 陈清远. 三叶青不同方式繁殖栽培技术试验[J]. 绿色科技,2019,21(17):120-123. |
| CHEN Q Y. Technical test of different propagation and cultivation methods of Tetrastigma hemsleyanum [J]. Journal of Green Science and Technology,2019,21(17):120-123. | |
| [5] | 胡春梅,李有清,赵富群,等. 三叶青无性扦插繁殖方法研究[J]. 南华大学学报(自然科学版),2018,32(5):86-91. |
| HU C M, LI Y Q, ZHAO F Q,et al. A study on asexual cutting propagating method of Tetrastigma hemsleyanum [J]. Journal of University of South China (Science and Technology),2018,32(5):86-91. | |
| [6] | 华金渭,朱波,吉庆勇,等. 不同种源三叶青生物学特性与扦插育苗试验[J]. 中国工业经济,2016(1):17-21. |
| HUA J W, ZHU B, JI Q Y,et al. Studies on biological characteristics and cuttage breeding technology of Tetrastigma hemsleyanum from different provenances[J]. China Industrial Economics 2016(1):17-21. | |
| [7] | 谢志亮,王盼,曾光辉,等. 三叶青嫩枝单芽扦插繁殖技术[J]. 浙江农业科学,2021,62(7):1394-1397. |
| XIE Z L, WANG P, ZENG G H,et al. Single-bud cutting propagation techniques of Tetrastigma hemsleyanum [J]. Journal of Zhejiang Agricultural Sciences,2021,62(7):1394-1397. | |
| [8] | 唐燕,谢志亮,陈松光,等. 三叶青水培扦插繁殖技术研究[J]. 浙江农业科学,2023,64(12):2883-2886. |
| TANG Y, XIE Z L, CHEN S G,et al. Study on cuttage propagation technology of Tetrastigma hemsleyanum in water culture[J]. Journal of Zhejiang Agricultural Sciences,2023,64(12):2883-2886. | |
| [9] | 吉庆勇,程文亮,华金渭,等. 三叶青扦插繁殖技术研究[J]. 现代中药研究与实践,2011,25(2):12-14. |
| JI Q Y, CHENG W L, HUA J W,et al. Study on cutting propagation techniques of Tetrastigma hemsleyanum [J]. Research and Practice on Chinese Medicines,2011,25(2):12-14. | |
| [10] | 阙建勇,叶邦宣,江丽娟,等. 浙西南地区三叶青扦插育苗技术研究[J]. 广西林业科学,2020,49(4):560-564. |
| QUE J Y, YE B X, JIANG L J,et al. Study on cuttage of Tetrastigma hemsleyanum in southwest Zhejiang[J]. Guangxi Forestry Science,2020,49(4):560-564. | |
| [11] | 方芳,邱智敏,王松,等. 扦插基质和插穗长度对三叶青扦插生根的影响[J]. 黑龙江农业科学,2020(9):85-87. |
| FANG F, QIU Z M, WANG S,et al. Effects of cutting medium and cutting length on rooting of Tetrastigma hemsleyanum [J]. Heilongjiang Agricultural Sciences,2020(9):85-87. | |
| [12] | 李振勤,李旭青,闫笑,等. 15个樱桃番茄品种单株产量与主要农艺性状的灰色关联分析及综合评价[J]. 中国农业大学学报,2024,29(11):63-73. |
| LI Z Q, LI X Q, YAN X,et al. Grey correlation analysis and comprehensive evaluation of the yield per plant and main agronomic characters of 15 cherry tomato varieties[J]. Journal of China Agricultural University,2024,29(11):63-73. | |
| [13] | 刘荣昌,徐红兵,余家中,等. 3种不同容器三叶青育苗技术试验初报[J]. 浙江农业科学,2022,63(4):705-707. |
| LIU R C, XU H B, YU J Z,et al. Preliminary report on Tetrastigma hemsleyanum seedling techniques in three different containers[J]. Journal of Zhejiang Agricultural Sciences,2022,63(4):705-707. | |
| [14] | 张沛健,高丽琼,尚秀华. 不同激素种类、浓度及浸泡时间对金蒲桃扦插生根的影响[J]. 热带作物学报,2020,41(6):1084-1091. |
| ZHANG P J, GAO L Q, SHANG X H. Effects of different hormone types,concentrations and soaking time on cutting rooting of Xanthostemon chrysanthus [J]. Chinese Journal of Tropical Crops,2020,41(6):1084-1091. | |
| [15] | 蔡晓郡,孙晗靖,陈雨姗,等. 三叶青种质资源农艺性状比较及聚类分析[J]. 分子植物育种,2022,20(16):5444-5453. |
| CAI X J, SUN H J, CHEN Y S,et al. Comparison and cluster analysis of agronomic characters of germplasm resources of Tetrastigma hemsleyanum [J]. Molecular Plant Breeding,2022,20(16):5444-5453. | |
| [16] | 张加强,骆霞虹,陈常理,等. 圆果种黄麻主要经济性状与纤维产量的相关及灰色关联分析[J]. 中国麻业科学,2015,37(2):70-74,79. |
| ZHANG J Q, LUO X H, CHEN C L,et al. Correlation and gray correlation between fiber yield and main economic traits of Corchorus capsularis L.[J]. Plant Fiber Sciences in China,2015,37(2):70-74,79. | |
| [17] | 赵斐斐,黎仿文,冀士俊,等. 基于层次分析(AHP)与灰色关联模型对大庆市野生花卉资源综合评价与筛选[J]. 现代园艺,2022,45(17):30-33. |
| ZHAO F F, LI F W, JI S J,et al. Comprehensive evaluation and screening of wild flower resources in Daqing City based on AHP and grey relational model[J]. Xiandai Horticulture,2022,45(17):30-33. | |
| [18] | 杨锦越,任洪,赵晓燕,等. 灰色关联分析和聚类分析在玉米品种综合评判中的应用[J]. 种子,2021,40(12):107-115. |
| YANG J Y, REN H, ZHAO X Y,et al. Application of grey correlation analysis and cluster analysis in comprehensive evaluation of maize varieties[J]. Seed,2021,40(12):107-115. | |
| [19] | 费云燕,余波,韩华新,等. 粳糯产量与产量构成因素的灰色关联度分析[J]. 大麦与谷类科学,2024,41(2):18-23. |
| FEI Y Y, YU B, HAN H X,et al. Grey correlation degree analysis of glutinous japonica rice yield and yield components[J]. Barley and Cereal Sciences,2024,41(2):18-23. |
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