ERF6基因在5种柑橘属植物的表达差异及启动子分析

doi:10.16178/j.issn.0528-9017.20180518

摘要/Abstract

摘要： 以柑橘属5种植物,枳、本地早、瓯柑、处红柚、佛手为材料,测定植物在低温胁迫下的抗寒性生理指标的变化,结合qRT-PCR实验检测ERF6基因在柑橘属不同物种响应低温的表达差异,并在佛手和枳中比较ERF6启动子序列结构。丙二醛含量测定结果表明,5种柑橘类植物抗寒性能力强弱顺序为枳>瓯柑>本地早>处红柚>佛手。qRT-PCR结果显示,ERF6基因表达量的上调与低温胁迫应答反应有关。对佛手、枳启动子的保守功能元件的分析结果表明,枳ERF6基因启动子与抗寒胁迫相关的顺式作用元件与佛手不同,推测可能是顺式元件的差异导致了低温胁迫能力的强弱。

关键词: 柑橘, 抗寒性, 启动子, 低温胁迫, ERF6

中图分类号:

S666

徐瑞, 叶杰君, 邬怡静, 何芯磊, 朱友银, 陈文荣, 郭卫东, 廖芳蕾. ERF6基因在5种柑橘属植物的表达差异及启动子分析[J]. 浙江农业科学, 2018, 59(5): 731-737.

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