浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (4): 813-818.DOI: 10.16178/j.issn.0528-9017.20231140

• 水稻 • 上一篇    下一篇

23份核心水稻材料稻瘟病和白叶枯病抗/感病基因的检测和分析

周慧(), 林祯芃, 周海平, 王成豹, 徐秀如, 钟林平, 马国华()   

  1. 温州市农业科学研究院 浙南作物育种重点实验室,浙江 温州 325006
  • 收稿日期:2023-11-29 出版日期:2025-04-11 发布日期:2025-05-09
  • 通讯作者: 马国华
  • 作者简介:马国华(1976—),男,浙江温州人,研究员,硕士,研究方向为水稻遗传育种,E-mail:392528617@qq.com。
    周慧(1987—),女,四川内江人,副研究员,博士,从事水稻遗传育种工作,E-mail:19yexiaoyun2006@163.com。
  • 基金资助:
    浙江省水稻农业新品种选育重大科技专项(2021C02063-2-4);浙江省农业关键核心技术攻关项目(GG01101-3);温州市国家农业科技园区开放性项目(K20200009);温州市农业新品种选育协作组项目(ZX2024001-2)

Detection and analysis of resistance/susceptibility genes of rice blast and bacterial blight in 23 core rice materials

ZHOU Hui(), LIN Zhenpeng, ZHOU Haiping, WANG Chengbao, XU Xiuru, ZHONG Linping, MA Guohua()   

  1. Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, Zhejiang
  • Received:2023-11-29 Online:2025-04-11 Published:2025-05-09
  • Contact: MA Guohua

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摘要：

培育抗病品种是防治水稻病虫害的最有效途径。为高效组配抗病新组合,利用17个稻瘟病和6个白叶枯病基因的分子标记对23份水稻核心材料进行抗/感病基因检测。稻瘟病抗/感病基因检测显示,抗病基因Pid2、Pi40和Ptr在检测材料中出现频次较高,分别为23、15和23份材料;抗病基因Pi64、Pib、Pi2、Pigm、Pi5、Pi1和Pikm的出现频次中等,分别为10、10、11、10、8、10和8份材料;抗病基因Pit、Pi36、Pikh、Pia和Pita仅存在于个别材料中,出现频次较少,分别为2、1、3、1和1份材料;抗病基因Piz和Pi25未在23份材料中检出。而感病基因pi2、piz、pi25、pikh、pikm、pia和pita在检测材料中高频次出现,分别为23、23、23、15、15、22和22份,pi64、pigm、pi36和pi5的检出频次中等,分别为9、13、13和13份。白叶枯病抗/感病基因检测表明,除5份材料中含Xa27抗病基因外,xa5、Xa7、xa13、Xa21和Xa23等抗病基因均未检测到;但Xa5、xa7、Xa13、xa21、xa23和xa27等感病基因出现的频率较高,分别是23、22、23、23、23和14。该研究利用23个已报道基因的分子标记检测23份水稻材料,初步确定了其稻瘟病和白叶枯病抗/感病基因的分布情况,为后续水稻材料的筛选、改良和测配组合提供了良好的遗传参考。

关键词: 水稻, 稻瘟病, 白叶枯病, 基因, 分子标记

Abstract:

Breeding disease-resistant varieties is the most effective approach to control rice diseases and pests. To efficiently assemble new disease-resistant combinations, molecular markers of 17 blast resistance genes and 6 bacterial blight resistance genes were used to detect resistance/susceptibility genes in 23 core rice materials. The blast resistance gene detection revealed that high-frequency resistance genes Pid2, Pi40 and Ptr were detected in 23, 15 and 23 materials respectively. Moderate-frequency genes Pi64, Pib, Pi2, Pigm, Pi5, Pi1 and Pikm occurred in 10, 10, 11, 10, 8, 10 and 8 materials respectively. Low-frequency genes Pit, Pi36, Pikh, Pia and Pita were only found in 2, 1, 3, 1 and 1 material. Piz and Pi25 were undetected. Conversely, susceptibility genes pi2, piz, pi25, pikh, pikm, pia and pita showed high frequencies (23, 23, 23, 15, 15, 22 and 22 materials respectively), while pi64, pigm, pi36 and pi5 exhibited moderate frequencies (9, 13, 13 and 13 materials). For bacterial blight genes, only resistance gene Xa27 was detected in 5 materials, while resistance genes xa5, Xa7, xa13, Xa21 and Xa23 were undetected. Susceptibility genes Xa5, xa7, Xa13, xa21, xa23 and xa27 showed high frequencies (23, 22, 23, 23, 23 and 14 materials respectively). This study systematically mapped the distribution of 23 reported resistance/susceptibility genes against rice blast and bacterial blight in 23 rice materials using molecular markers, providing valuable genetic references for subsequent screening, improvement and combination testing of rice germplasm.

Key words: rice, rice blast, bacterial blight, gene, molecular maker

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