日粮能量水平对猪TCF7L2基因表达的影响

doi:10.16178/j.issn.0528-9017.20231112

摘要/Abstract

摘要：

本研究旨在探讨日粮能量水平对长白猪转录因子7类似物2基因(transcription factor 7-like 2 gene, TCF7L2)mRNA相对表达量的影响, 选用同日出生的90日龄长白猪24头, 按日粮能量水平分成2组, 饲喂至180 d, 统一屠宰取样。采用实时荧光定量PCR(qRT-PCR)方法检测2组长白猪皮下脂肪和背最长肌中TCF7L2基因mRNA的相对表达量,并测定血液生化指标。结果表明,TCF7L2基因在两组试验猪组织间的表达模式基本相似,在皮下脂肪中的表达量高于背最长肌;组间的比较结果显示,H组(高能量组)皮下脂肪和背最长肌中TCF7L2基因mRNA表达量高于L组。日粮能量水平可以影响猪TCF7L2基因mRNA的表达水平和血液生化指标,进而影响皮下脂肪和肌内脂肪沉积,为深入研究猪脂肪代谢的调控机理提供依据。

关键词: 猪, 脂肪, TCF7L2, 日粮能量水平

Abstract:

This experiment was conducted to study the effect of dietary energy level on transcription factor 7-like 2 gene (TCF7L2) mRNA expression in Landrace pigs. Twenty four Landrace pigs (90 days old) were divided into two groups according to the dietary energy level, slaughtered and sampled when they were 180 days. The expressions of TCF7L2 mRNA in these two Landrace pig groups was measured by qRT-PCR, and blood biochemical indicators were measured. The results showed that the expression patterns of TCF7L2 mRNA in these two groups were similar, in which back fat was great higher than longissimus dorsi muscle. Comparison between these two groups indicated that the expression of TCF7L2 mRNA in back fat and longissimus dorsi muscle in H(high energy) group was higher than that in L (low energy) group. The results suggested that dietary energy level could influence the expression level of TCF7L2 mRNA and blood biochemical indicators in pigs, thereby affecting subcutaneous and intramuscular fat deposition, which provided reference for further study on the molecular mechanism of fat metabolism in pig.

Key words: pig, fat, TCF7L2, dietary energy level

中图分类号:

S828

李庆海, 张雷, 范京辉, 葛莹, 魏莹晖, 刘航, 王欢欢, 王彦芦, 张伟武. 日粮能量水平对猪TCF7L2基因表达的影响[J]. 浙江农业科学, 2024, 65(12): 3035-3038.

LI Qinghai, ZHANG Lei, FAN Jinghui, GE Ying, WEI Yinghui, LIU Hang, WANG Huanhuan, WANG Yanlu, ZHANG Weiwu. Effects of dietary energy level on TCF7L2 gene expression in pigs[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(12): 3035-3038.

图/表 5

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时期	级别	玉米/ %	麦麸/ %	豆粕/ %	植物油/ %	石粉/ %	磷酸氢钙/ %	赖氨酸/ %	食盐/ %	预混料/ %	消化能/ (MJ·kg^-1)	粗蛋白/ %	钙/ %	总磷/ %
生长期	H	70.71	0	24.71	1.69	0.75	0.80	0.04	0.30	1.00	14.22	16.40	0.55	0.48
	L	51.55	26.19	19.52	0	1.00	0.41	0.03	0.30	1.00	12.55	16.40	0.55	0.57
肥育期	H	76.29	0	19.40	1.60	0.76	0.60	0.05	0.30	1.00	14.22	14.50	0.49	0.43
	L	56.59	26.80	14.10	0	1.11	0.10	0	0.30	1.00	12.55	14.50	0.49	0.50

时期	级别	玉米/ %	麦麸/ %	豆粕/ %	植物油/ %	石粉/ %	磷酸氢钙/ %	赖氨酸/ %	食盐/ %	预混料/ %	消化能/ (MJ·kg^-1)	粗蛋白/ %	钙/ %	总磷/ %
生长期	H	70.71	0	24.71	1.69	0.75	0.80	0.04	0.30	1.00	14.22	16.40	0.55	0.48
	L	51.55	26.19	19.52	0	1.00	0.41	0.03	0.30	1.00	12.55	16.40	0.55	0.57
肥育期	H	76.29	0	19.40	1.60	0.76	0.60	0.05	0.30	1.00	14.22	14.50	0.49	0.43
	L	56.59	26.80	14.10	0	1.11	0.10	0	0.30	1.00	12.55	14.50	0.49	0.50

基因名称	基因序列号	引物序列(5'-3')	扩增长度/bp	熔解温度/℃
Pig TCF7L2	XM_001928695.3	CCCACCGCCCGAACCTATCT	144	64
		GTGTTGCACAACCGGCACTTTG
Pig 18S	NR_046261	GCCCTATCAACTTTCGATGGTAGTC	113	64
		CCTTGGATGTGGTAGCCGTTTCTCA

基因名称	基因序列号	引物序列(5'-3')	扩增长度/bp	熔解温度/℃
Pig TCF7L2	XM_001928695.3	CCCACCGCCCGAACCTATCT	144	64
		GTGTTGCACAACCGGCACTTTG
Pig 18S	NR_046261	GCCCTATCAACTTTCGATGGTAGTC	113	64
		CCTTGGATGTGGTAGCCGTTTCTCA

组别	皮下脂肪	背最长肌
H	41.75±6.32^**	7.27±1.11
L	34.38±5.80	5.45±1.05