连云港卷丹百合组织培养技术初探

doi:10.16178/j.issn.0528-9017.20250657

摘要/Abstract

摘要：

卷丹百合是连云港云台山脉特用药食兼用百合品种。由于附近居民的盗挖及牛羊的啃食,该物种已处于灭绝边缘。为了建立快繁技术体系,本研究以百合鳞片为外植体,发现用75%乙醇消毒30 s,再用2%NaClO消毒10 min的消毒处理较为合适。适宜不定芽诱导的培养基为MS+45 g·L^-1蔗糖+0.1 mg·L^-1萘乙酸(NAA)+ 1.0 mg·L^-1 6-苄氨基嘌呤(6-BA);适宜不定芽生根的培养基为 MS+0.3 mg·L^-1 NAA。

关键词: 卷丹百合, 组织培养, 不定芽, 培养基

Abstract:

Lilium lancifolium Thunb. is a special type of lily used in the Yuntai Mountains of Lianyungang. Due to thefts by nearby residents and gnawing by cattle and sheep, the species are on the verge of extinction. In order to establish a rapid breeding technology system, this study used scales of Lilium lancifolium Thunb. as explants. It was found that the disinfection treatment of treating scales with 75% ethanol for 30 seconds and 2% NaClO for 10 minutes was more appropriate. The appropriate culture medium for induction of adventitious buds was MS+45 g·L^-1 sucrose +0.1 mg·L^-1NAA + 1.0 mg·L^-1 6-BA. The appropriate culture medium for rooting of adventitious buds was MS+0.3 mg·L^-1NAA.

Key words: Lilium lancifolium Thunb., tissue culture, adventitious bud, culture medium

中图分类号:

S682.265

封士达, 葛金涛, 金东林, 徐艳, 王多明, 邵小斌. 连云港卷丹百合组织培养技术初探[J]. 浙江农业科学, 2026, 67(1): 47-51.

FENG Shida, GE Jintao, JIN Donglin, XU Yan, WANG Duoming, SHAO Xiaobin. Preliminary study on tissue culture technology of Lilium lancifolium Thunb. in Lianyungang[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(1): 47-51.

图/表 4

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处理	乙醇消毒时间/s	NaClO体积分数/%	NaClO消毒时间/min	接种数	外植体污染率/%	褐化率/%
CK	30	0	—	120	96.67±3.33 a	0 f
1	30	2	5	120	52.22±3.84 b	5.56±1.92 e
2	30	2	10	120	20.00±3.33 d	16.66±0 d
3	30	2	15	120	13.33±3.33 e	38.89±1.92 b
4	30	3	5	120	32.22±3.84 c	21.11±1.92 c
5	30	3	10	120	5.55±1.92 f	41.11±1.92 b
6	30	3	15	120	4.44±1.92 f	66.33±1.92 a

处理	乙醇消毒时间/s	NaClO体积分数/%	NaClO消毒时间/min	接种数	外植体污染率/%	褐化率/%
CK	30	0	—	120	96.67±3.33 a	0 f
1	30	2	5	120	52.22±3.84 b	5.56±1.92 e
2	30	2	10	120	20.00±3.33 d	16.66±0 d
3	30	2	15	120	13.33±3.33 e	38.89±1.92 b
4	30	3	5	120	32.22±3.84 c	21.11±1.92 c
5	30	3	10	120	5.55±1.92 f	41.11±1.92 b
6	30	3	15	120	4.44±1.92 f	66.33±1.92 a

处理	NAA质量浓度/(mg·L^-1)	6-BA质量浓度/(mg·L^-1)	接种数	不定芽诱导率/%	单个鳞片诱导芽数
1	0.1	0	120	58.33±4.30 d	1.50±0.57 e
2	0.1	0.5	120	80.83±3.19 b	2.50±0.57 d
3	0.1	1.0	120	93.33±2.72 a	7.50±0.57 a
4	0.1	1.5	120	71.67±1.92 c	4.50±0.57 b
5	0.1	2.0	120	53.33±2.72 e	3.75±0.50 bc
6	0.1	2.5	120	23.33±2.72 f	3.25±0.50 c

处理	NAA质量浓度/(mg·L^-1)	6-BA质量浓度/(mg·L^-1)	接种数	不定芽诱导率/%	单个鳞片诱导芽数
1	0.1	0	120	58.33±4.30 d	1.50±0.57 e
2	0.1	0.5	120	80.83±3.19 b	2.50±0.57 d
3	0.1	1.0	120	93.33±2.72 a	7.50±0.57 a
4	0.1	1.5	120	71.67±1.92 c	4.50±0.57 b
5	0.1	2.0	120	53.33±2.72 e	3.75±0.50 bc
6	0.1	2.5	120	23.33±2.72 f	3.25±0.50 c

处理	6-BA质量浓度/ (mg·L^-1)	蔗糖质量浓度/ (g·L^-1)	不定芽诱导率/%	单个鳞片诱导芽数	鳞片发育状态
1	1.0	0	17.50±3.10 e	1.00±0.81 d	大部分褐化,小部分颜色为褐黄绿色且没有芽点
2	1.0	15	86.67±2.70 c	4.00±0.81 c	芽点较少,发育较慢
3	1.0	30	91.67±1.90 b	7.75±0.96 b	芽点较多,生长相对较慢
4	1.0	45	96.67±2.70 a	9.25±0.96 a	芽点较多,生长较快且芽饱满
5	1.0	60	63.33±2.70 d	4.75±0.96 c	褐化率严重,芽畸形率较高