高效液相色谱法同时检测百菌清和环丙唑醇

doi:10.16178/j.issn.0528-9017.20230849

摘要/Abstract

摘要：

百菌清和环丙唑醇都是高效的杀菌剂,但目前还没有同时检测这两种成分的分析方法。该研究为建立可以同时检测百菌清和环丙唑醇的分析方法,采用高效液相色谱仪配置ODS-3 C18色谱柱和紫外检测器,以甲醇和水为流动相,在224 nm波长下,对40.5%百菌清·环丙唑醇悬浮剂中百菌清和环丙唑醇进行分离和定量分析。结果表明,该方法对百菌清、环丙唑醇的线性相关系数分别为0.999 7和0.999 9,标准偏差分别为0.132 0和0.013 7,变异系数分别为0.35%和0.45%,平均回收率分别为99.34%和99.62%。该方法具有良好的精密度和准确度,可以同时检测40.5%百菌清·环丙唑醇悬浮剂中的百菌清和环丙唑醇,且适用于相关产品的检测。

关键词: 百菌清, 环丙唑醇, 悬浮剂, 高效液相色谱, 分析

Abstract:

Chlorothalonil and cyproconazole are both efficient fungicides, but there is currently no analytical method for simultaneously detecting these two components. This study aims to establish an analytical method that can simultaneously detect chlorothalonil and cyproconazole. A high-performance liquid chromatography (HPLC) was used to configure an ODS-3 C18 column and a UV detector. Methanol and water were used as mobile phases, and chlorothalonil and cyproconazole were separated and quantitatively analyzed in a 40.5% chlorothalonil cyproconazole suspension at a wavelength of 224 nm. The results showed that the linear correlation coefficients of this method for chlorothalonil and cyproconazole were 0.999 7 and 0.999 9, respectively, with standard deviations of 0.132 0 and 0.013 7, coefficient of variation of 0.35% and 0.45%, and average recovery rates of 99.34% and 99.62%, respectively. This method has good precision and accuracy, and can simultaneously detect chlorothalonil and cyproconazole in 40.5% suspension of chlorothalonil and cyproconazole, and is suitable for the detection of related products.

Key words: chlorothalonil, cyproconazole, suspension, high-performance liquid chromatography, analysis

中图分类号:

S482.2

龚国华. 高效液相色谱法同时检测百菌清和环丙唑醇[J]. 浙江农业科学, 2024, 65(8): 1923-1928.

GONG Guohua. Simultaneous detection of chlorothalonil and cyproconazole by high-performance liquid chromatography[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(8): 1923-1928.

图/表 8

图1 百菌清的化学结构式

Fig.1 Chemical structural formula of chlorothalonil

图2 环丙唑醇4种立体异构体的化学结构式

Fig.2 Chemical structural formulas of four stereoisomers of cyproconazole

图3 百菌清、环丙唑醇标样与40.5%百菌清·环丙唑醇悬浮剂的液相色谱图

Fig.3 Liquid chromatography of chlorothalonil, cyproconazole standard sample and 40.5% suspension of chlorothalonil and cyproconazole

图4 百菌清的标准曲线

Fig.4 Standard curve of chlorothalonil

图5 环丙唑醇的标准曲线

Fig.5 Standard curve of cyproconazole

图6 标样和试样中百菌清和环丙唑醇的纯度图

Fig.6 Purity diagram of chlorothalonil and cyproconazole in standard and test samples

表1 分析方法的精密度试验结果

Table 1 Precision test results of analytical method

有效成分	质量分数/%						标准偏差	变异系数/%
有效成分	1	2	3	4	5	平均值	标准偏差	变异系数/%
百菌清	37.2	37.36	37.31	37.56	37.37	37.36	0.132 0	0.35
环丙唑醇	3.02	3.03	3.02	3.05	3.04	3.03	0.013 7	0.45

表2 分析方法的准确度试验结果

Table 2 Accuracy test results of analytical method

有效成分	编号	标样加入量/mg	实测值/ mg	回收率/ %	平均回收率/%
百菌清	1	17.54	17.38	99.08	99.34
	2	17.54	17.38	99.10
	3	17.54	17.49	99.74
	4	17.54	17.41	99.28
	5	17.54	17.45	99.48
环丙唑醇	1	1.59	1.58	99.20	99.62
	2	1.59	1.57	98.57
	3	1.59	1.57	98.85
	4	1.59	1.59	100.08
	5	1.59	1.61	101.38

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