浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (5): 1190-1194.DOI: 10.16178/j.issn.0528-9017.20240665

• 药用植物 • 上一篇    下一篇

激素配比对蛇果黄堇组培苗增殖及生根诱导的影响

李倩1(), 黄丽容1, 韦莹1, 雷明1, 张占江1, 周翠云2, 潘丽梅1, 李翠1,*()   

  1. 1.广西壮族自治区药用植物园 国家中医药传承创新中心 广西药用资源保护与遗传改良重点实验室 广西壮族自治区中药资源智慧创制工程研究中心 广西道地药材高品质形成与应用重点实验室, 广西 南宁 530023
    2.广西中医药大学 赛恩斯新医药学院, 广西 南宁 530023
  • 收稿日期:2024-08-19 出版日期:2025-05-11 发布日期:2025-05-20
  • 通讯作者: 李翠(1982—),女,主任技师,硕士,研究方向为药用植物资源开发利用,成果:药用植物资源保育研究,E-mail:licuicui941@163.com。
  • 作者简介:李倩(1985—),女,工程师,学士,主要研究方向为药用植物繁育研究,E-mail:358214604@qq.com。
  • 基金资助:
    广西重点研发计划(桂科AB23026092);广西自然科学基金面上项目(2023GXNSFAA026509);广西自然科学基金面上项目(2020GXNSFAA159151);广西中医药管理局广西特色药材关键技术研究推广项目(GZKJ2314);广西中医药适宜技术开发与推广项目(GZSY22-02);广西药用资源保护与遗传改良重点实验室项目(KL2022ZZ04);广西药用资源保护与遗传改良重点实验室项目(KL2023ZZ09);广西药用资源保护与遗传改良重点实验室开放课题(KL2023KF01);广西药用资源保护与遗传改良重点实验室开放课题(KL2023KF04)

Effect of hormone ratio on proliferation and rooting induction of tissue culture seedlings of Corydalis ophiocarpa Hook. f. et Thoms.

LI Qian1(), HUANG Lirong1, WEI Ying1, LEI Ming1, ZHANG Zhanjiang1, ZHOU Cuiyun2, PAN Limei1, LI Cui1,*()   

  1. 1. National Traditional Chinese Medicine Inheritance and Innovation Center,Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Traditional Chinese Medicine Resources Intelligent Creation Engineering Research Center,Guangxi Key Laboratory of High Quality Formation and Application of Genuine Medicinal Materials, Guangxi Zhuang Autonomous Region Medicinal Botanical Garden, Nanning 530023, Guangxi
    2. Faculty of Chinese Medicine Science Guangxi University of Chinese Medicine, Nanning 530023, Guangxi
  • Received:2024-08-19 Online:2025-05-11 Published:2025-05-20

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摘要： 为研究不同激素配比对蛇果黄堇组培苗增殖及生根诱导的影响,使用MS为基本培养基,采用吲哚丁酸(IBA,0.2, 0.5, 1.0 mg·L-1)、吲哚乙酸(IAA,0.1, 0.2, 0.5 mg·L-1)、6-苄基腺嘌呤(6-BA,0.3, 0.6, 1.0 mg·L-1)进行三水平三因素正交试验,筛选最佳增殖培养基;采用NAA(0.5, 1.0, 2.0 mg·L-1)、IBA (0.5, 1.0, 2.0 mg·L-1),筛选诱导生根的最佳培养基。结果表明,增殖和诱导培养基以MS+0.2 mg·L-1IBA+0.1 mg·L-1IAA+ 0.3 mg·L-16-BA增殖效果最佳,增殖倍数在8倍以上,且适宜用于蛇果黄堇分化不定芽,能有效增加苗株数量;MS+0.5 mg·L-1NAA(pH值5.8)是蛇果黄堇主根生根的最佳培养基,MS+1.0 mg·L-1IBA(pH值5.8)是蛇果黄堇须根生根的最佳培养基。本研究确定了适宜蛇果黄堇增殖和生根的最佳培养基配方,显著提高了蛇果黄堇的增殖效率和生根质量,为蛇果黄堇的高效繁殖提供了重要基础。

关键词: 蛇果黄堇, 组织培养, 增殖, 生根

Abstract:

To investigate the effects of different hormone ratios on the proliferation and rooting induction of tissue culture seedlings of Corydalis ophiocarpa Hook. f. et Thoms. MS was used as the basic medium, IBA(0.2, 0.5, 1.0 mg·L-1), IAA(0.1, 0.2, 0.5 mg·L-1), and 6-BA(0.3, 0.6, 1.0 mg·L-1) were tested by three-level and three-factor orthogonal test to screen the best proliferation medium. NAA(0.5, 1.0, 2.0 mg·L-1) and IBA(0.5, 1.0, 2.0 mg·L-1) were used to select the best medium for inducing rooting. The results showed that the culture medium MS+0.2 mg·L-1 IBA+0.1 mg·L-1 IAA+0.3 mg·L-1 6-BA had the best proliferation effect, with a multiplication rate of more than 8 times, and was suitable for inducing the differentiation of adventitious buds in Corydalis ophiocarpa Hook. f. et Thoms., effectively increasing the number of seedlings. MS+0.5 mg·L-1 NAA(pH value 5.8) was identified as the optimal culture medium for the rooting of main roots in Corydalis ophiocarpa Hook. f. et Thoms., while MS+1.0 mg·L-1 IBA(pH value 5.8) was the optimal culture medium for the rooting of fibrous roots. This study has determined the best culture medium formulas for the proliferation and rooting of Corydalis ophiocarpa Hook. f. et Thoms., significantly improving the efficiency and quality of rooting, providing an important basis for efficient propagation of Corydalis ophiocarpa Hook. f. et Thoms..

Key words: Corydalis ophiocarpa Hook. f. et Thoms, tissue culture, proliferation, rooting

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