Abstract:

The microorganisms on the surface and inside of tobacco leaves are rich and diverse，which are closely related to the aging quality of tobacco leaves. In this study，the differential microbial proteins and source microorganisms of tobacco leaves with different aging time were analyzed by macroproteomics technology，and the enzymes related to improving the quality of tobacco leaves were excavated to identify the key functional microorganisms. The results showed that the microbial proteins of tobacco leaves with different aging time were significantly （p<0.05） different. During the aging process of tobacco leaves，Acinetobacter and Sphingomonas synergistically degraded tobacco cellulose through β-1，4-glucanase and β-glucosidase. Pseudomonas facilitated protein degradation in tobacco leaves by producing aminopeptidase，while Pseudomonas and Sphingomonas produced β-amylase and glucoamylase to degrade starch synergistically. In addition，dominant genera such as Pseudomonas，Acinetobacter，and Enterobacter were involved in the degradation of carotenoids and their derivatives. This study provides a theoretical basis for the subsequent improvement of tobacco leaves aging strategies.

Key words: tobacco leaf aging, macroproteomic, differential microbial protein, functional microorganism