Preliminary study on tissue culture technology of Lilium lancifolium Thunb. in Lianyungang

doi:10.16178/j.issn.0528-9017.20250657

Abstract

Abstract:

Lilium lancifolium Thunb. is a special type of lily used in the Yuntai Mountains of Lianyungang. Due to thefts by nearby residents and gnawing by cattle and sheep, the species are on the verge of extinction. In order to establish a rapid breeding technology system, this study used scales of Lilium lancifolium Thunb. as explants. It was found that the disinfection treatment of treating scales with 75% ethanol for 30 seconds and 2% NaClO for 10 minutes was more appropriate. The appropriate culture medium for induction of adventitious buds was MS+45 g·L^-1 sucrose +0.1 mg·L^-1NAA + 1.0 mg·L^-1 6-BA. The appropriate culture medium for rooting of adventitious buds was MS+0.3 mg·L^-1NAA.

Key words: Lilium lancifolium Thunb., tissue culture, adventitious bud, culture medium

CLC Number:

S682.265

FENG Shida, GE Jintao, JIN Donglin, XU Yan, WANG Duoming, SHAO Xiaobin. Preliminary study on tissue culture technology of Lilium lancifolium Thunb. in Lianyungang[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(1): 47-51.

Figures/Tables 4

References 15

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处理	乙醇消毒时间/s	NaClO体积分数/%	NaClO消毒时间/min	接种数	外植体污染率/%	褐化率/%
CK	30	0	—	120	96.67±3.33 a	0 f
1	30	2	5	120	52.22±3.84 b	5.56±1.92 e
2	30	2	10	120	20.00±3.33 d	16.66±0 d
3	30	2	15	120	13.33±3.33 e	38.89±1.92 b
4	30	3	5	120	32.22±3.84 c	21.11±1.92 c
5	30	3	10	120	5.55±1.92 f	41.11±1.92 b
6	30	3	15	120	4.44±1.92 f	66.33±1.92 a

处理	乙醇消毒时间/s	NaClO体积分数/%	NaClO消毒时间/min	接种数	外植体污染率/%	褐化率/%
CK	30	0	—	120	96.67±3.33 a	0 f
1	30	2	5	120	52.22±3.84 b	5.56±1.92 e
2	30	2	10	120	20.00±3.33 d	16.66±0 d
3	30	2	15	120	13.33±3.33 e	38.89±1.92 b
4	30	3	5	120	32.22±3.84 c	21.11±1.92 c
5	30	3	10	120	5.55±1.92 f	41.11±1.92 b
6	30	3	15	120	4.44±1.92 f	66.33±1.92 a

处理	NAA质量浓度/(mg·L^-1)	6-BA质量浓度/(mg·L^-1)	接种数	不定芽诱导率/%	单个鳞片诱导芽数
1	0.1	0	120	58.33±4.30 d	1.50±0.57 e
2	0.1	0.5	120	80.83±3.19 b	2.50±0.57 d
3	0.1	1.0	120	93.33±2.72 a	7.50±0.57 a
4	0.1	1.5	120	71.67±1.92 c	4.50±0.57 b
5	0.1	2.0	120	53.33±2.72 e	3.75±0.50 bc
6	0.1	2.5	120	23.33±2.72 f	3.25±0.50 c

处理	NAA质量浓度/(mg·L^-1)	6-BA质量浓度/(mg·L^-1)	接种数	不定芽诱导率/%	单个鳞片诱导芽数
1	0.1	0	120	58.33±4.30 d	1.50±0.57 e
2	0.1	0.5	120	80.83±3.19 b	2.50±0.57 d
3	0.1	1.0	120	93.33±2.72 a	7.50±0.57 a
4	0.1	1.5	120	71.67±1.92 c	4.50±0.57 b
5	0.1	2.0	120	53.33±2.72 e	3.75±0.50 bc
6	0.1	2.5	120	23.33±2.72 f	3.25±0.50 c

处理	6-BA质量浓度/ (mg·L^-1)	蔗糖质量浓度/ (g·L^-1)	不定芽诱导率/%	单个鳞片诱导芽数	鳞片发育状态
1	1.0	0	17.50±3.10 e	1.00±0.81 d	大部分褐化,小部分颜色为褐黄绿色且没有芽点
2	1.0	15	86.67±2.70 c	4.00±0.81 c	芽点较少,发育较慢
3	1.0	30	91.67±1.90 b	7.75±0.96 b	芽点较多,生长相对较慢
4	1.0	45	96.67±2.70 a	9.25±0.96 a	芽点较多,生长较快且芽饱满
5	1.0	60	63.33±2.70 d	4.75±0.96 c	褐化率严重,芽畸形率较高