Journal of Zhejiang Agricultural Sciences ›› 2026, Vol. 67 ›› Issue (5): 1264-1268.DOI: 10.16178/j.issn.0528-9017.20250184

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A preliminary test of open-field overwintering cultivation of 30 Hippeastrum cultivars in Hangzhou and its tissue culture techniques

YU Shuijun1(), FU Jialiang1(), YU Qing2, WANG Jiajie1, PAN Liming3, NI Jianfeng4, XU Jinming5   

  1. 1.Agricultural and Forestry Technology Extension Center of Xiaoshan District,Hangzhou City,Hangzhou 311203,Zhejiang
    2.Hangzhou Shulian Ecological Engineering Co. ,Ltd. ,Hangzhou 311200,Zhejiang
    3.Economic Cooperative of Guanyi Village,Guali Town,Xiaoshan District,Hangzhou City,Hangzhou 311245,Zhejiang
    4.Economic Cooperative of Dangshan Village,Guali Town,Xiaoshan District,Hangzhou City,Hangzhou 311245,Zhejiang
    5.Economic Cooperative of Shanmuqiao Village,Guali Town,Xiaoshan District,Hangzhou City,Hangzhou 311245,Zhejiang
  • Received:2025-03-12 Online:2026-05-11 Published:2026-05-12
  • Contact: FU Jialiang

Abstract:

30 Hippeastrum cultivars were introduced as experimental materials and cultivated outdoors in Hangzhou with no protective measures during winter. Regular observations were conducted to screen cultivars suitable for open-field cultivation in this region. Using bulbs as explants,the effects of different ratios of plant hormone on adventitious bud induction and subculture proliferation of Hippeastrum were investigated. The results showed that Nanhai Puella,Double Dragon,Blossom Peacock,Alfresco,Amadeus and Dancing Queen six cultivars exhibited strong cold tolerance and were suitable for open-field cultivation in Hangzhou. The optimal medium for adventitious bud induction of Hippeastrum Alfresco was MS + 1.0 mg·L-1 NAA + 2.0 mg·L-1 6-BA + 3% sucrose + 5 g·L-1 agar,while the optimal medium for subculture proliferation of Hippeastrum Alfresco was MS + 0.5 mg·L-1 NAA+ 4.0 mg·L-1 6-BA + 3% sucrose + 5 g·L-1 agar. For Hippeastrum Double Dragon,the optimal medium for adventitious bud induction was MS + 2.0 mg·L-1 NAA+ 2.5 mg·L-1 6-BA + 3% sucrose + 5 g·L-1 agar,and the optimal medium for subculture proliferation was MS + 0.5 mg·L-1NAA+2.0 mg·L-1 6-BA + 3% sucrose + 5 g·L-1 agar.

Key words: Hippeastrum, open-field cultivation, cold resistance, tissue culture

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