扦插后白花真樱免疫响应的转录组分析

doi:10.16178/j.issn.0528-9017.20250553

摘要/Abstract

摘要：

白花真樱（Prunus lannesiana cv. Multiplex），是目前华南地区嫁接樱花的首选砧木，一般通过扦插进行繁殖。在扦插过程中，伤口直接暴露在外，为病原菌提供了有利的侵染条件，推测免疫基因在根再生中发挥重要作用。本研究发现5个富含亮氨酸重复序列基因表达显著上调，4个丝裂原活化蛋白激酶基因表达显著上调，以及1个凝集素基因和2个水杨酸基因表达显著上调。通过转录组技术解析白花真樱扦插生根的免疫响应模式，可以了解免疫基因在白花真樱扦插生根过程中的作用，从而为提高插穗成活率提供理论基础。

关键词: 白花真樱, 免疫, 模式识别受体

Abstract:

Prunus lannesiana cv. Multiplex is currently the preferred rootstock for grafting cherry blossoms in southern China. It is usually propagated through cuttings. During the cutting process，the wound is directly exposed，providing favorable infection conditions for pathogens. It was speculated that immune genes play an important role in root regeneration. This study found that 5 LRR genes were significantly upregulated，4 mitogen activated protein kinase genes were significantly upregulated，and the expression levels of 1 lectin gene and 2 salicylic acid genes were significantly upregulated. By using transcriptome technology to analyze the immune response patterns of P. lannesiana cutting rooting，we aim to understand the role of immune genes in the rooting process of P. lannesiana cutting，and provide a theoretical basis for improving the survival rate of cuttings.

Key words: Prunus lannesiana cv. Multiplex, immune, pattern recognition receptor

中图分类号:

S682.3

丰凯, 于飞龙, 李文, 刘亮, 赵一阳, 王志龙. 扦插后白花真樱免疫响应的转录组分析[J]. 浙江农业科学, 2026, 67(2): 424-430.

FENG Kai, YU Feilong, LI Wen, LIU Liang, ZHAO Yiyang, WANG Zhilong. Transcriptomic analysis of immune responses in Prunus lannesiana cv. Multiplex after cuttage[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(2): 424-430.

图/表 9

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基因编号	正向引物（5′?3′）	反向引物（5′?3′）
gene-Pyn_01444	AGGGATGCGGCTTCTATTTC	TCCAACTCCAGCTCCTCTAT
gene-Pyn_02031	CGGGATTGTGAGGAGGTTTAT	ATAAGTCGAGCCACCCTTTC
gene-Pyn_03059	GGGTTGATTGCGAGATTGTTG	GCCACTGAGTCACTCGTAAAT
gene-Pyn_04995	GCACAAGTCATGGAAGGAGATA	CAGAGCTTCCACCTTCATCTAC
gene-Pyn_11765	CTCGCAATAAGAGGCCATAGAA	AGGAACAGTGACTGAACAGAAG
gene-Pyn_15160	CCGCTCTGTCCATCATCTTTAC	AGCAGCAACAGGAGCAATAC
gene-Pyn_18392	GCCCAACATCAGAAACATCATC	GCACAGAAGAAATCAACCACTATC
gene-Pyn_19342	CCATGGTCCCTTCAGCTTATT	TTGGGTGGCTTTGCTTCT
gene-Pyn_26801	GTGAGCTTCATCTGGGTACTTC	GCACTATCAACCCTCTGTCTTT
gene-Pyn_30574	GGAGAAGGAGAAGAAGAATGGAG	GAGCTATGAGCTTGGAGAGATG
gene-Pyn_31583	CAGCAACCATAGCCAACAAATAC	TCTGAGGAGGTCCAAGTGATAG
gene-Pyn_31585	GGAAGTGGCAGAGAAGATCAG	CTGTCTGATGACCCACCAATAG
gene-Pyn_31584	CCATGGTGCTCTCAGATTAAGG	GGAACACCACACCACATACTT
gene-Pyn_31998	GGACTCTGAAACTGGTAGAAAGG	CAAGAGTGGGCCTATTGGTAAA
gene-Pyn_34281	GACCACTTCCGCTTCGTTTA	GAACCTTCTCGGCGATCTTAG
gene-Pyn_36772	AGCTTCCTTGTCCCTCAAAC	CTCTGCAGTCCATCCAAAGAA
gene-Pyn_38400	CAAGGAGGGTGCTGAATCTT	CACGGGAGGAGTTTGACTTT
gene-Pyn_40439	AGGACAGCGTTGGTGATAAG	AGAACTGGAAGACCAGCATAAA
gene-Pyn_40440	GCTTTGCCTGTCTACCCTATT	CTGACTGTTTGGGCTGAGAA
gene-Pyn_35098	GGGCTCCAAATCCGGTTAAT	GATCAGGTCTTGTGAAGGTAGC
gene-Pyn_21690	GCAAAGGAAGGGTTTGAGATTG	TGGGTGTGGATGATGGTTATG
PlACTIN	AAGGCTGGGTTTGCTGGAGATGAT	GACCACTGGCGTAGAGGGAAAGAA

基因编号	正向引物（5′?3′）	反向引物（5′?3′）
gene-Pyn_01444	AGGGATGCGGCTTCTATTTC	TCCAACTCCAGCTCCTCTAT
gene-Pyn_02031	CGGGATTGTGAGGAGGTTTAT	ATAAGTCGAGCCACCCTTTC
gene-Pyn_03059	GGGTTGATTGCGAGATTGTTG	GCCACTGAGTCACTCGTAAAT
gene-Pyn_04995	GCACAAGTCATGGAAGGAGATA	CAGAGCTTCCACCTTCATCTAC
gene-Pyn_11765	CTCGCAATAAGAGGCCATAGAA	AGGAACAGTGACTGAACAGAAG
gene-Pyn_15160	CCGCTCTGTCCATCATCTTTAC	AGCAGCAACAGGAGCAATAC
gene-Pyn_18392	GCCCAACATCAGAAACATCATC	GCACAGAAGAAATCAACCACTATC
gene-Pyn_19342	CCATGGTCCCTTCAGCTTATT	TTGGGTGGCTTTGCTTCT
gene-Pyn_26801	GTGAGCTTCATCTGGGTACTTC	GCACTATCAACCCTCTGTCTTT
gene-Pyn_30574	GGAGAAGGAGAAGAAGAATGGAG	GAGCTATGAGCTTGGAGAGATG
gene-Pyn_31583	CAGCAACCATAGCCAACAAATAC	TCTGAGGAGGTCCAAGTGATAG
gene-Pyn_31585	GGAAGTGGCAGAGAAGATCAG	CTGTCTGATGACCCACCAATAG
gene-Pyn_31584	CCATGGTGCTCTCAGATTAAGG	GGAACACCACACCACATACTT
gene-Pyn_31998	GGACTCTGAAACTGGTAGAAAGG	CAAGAGTGGGCCTATTGGTAAA
gene-Pyn_34281	GACCACTTCCGCTTCGTTTA	GAACCTTCTCGGCGATCTTAG
gene-Pyn_36772	AGCTTCCTTGTCCCTCAAAC	CTCTGCAGTCCATCCAAAGAA
gene-Pyn_38400	CAAGGAGGGTGCTGAATCTT	CACGGGAGGAGTTTGACTTT
gene-Pyn_40439	AGGACAGCGTTGGTGATAAG	AGAACTGGAAGACCAGCATAAA
gene-Pyn_40440	GCTTTGCCTGTCTACCCTATT	CTGACTGTTTGGGCTGAGAA
gene-Pyn_35098	GGGCTCCAAATCCGGTTAAT	GATCAGGTCTTGTGAAGGTAGC
gene-Pyn_21690	GCAAAGGAAGGGTTTGAGATTG	TGGGTGTGGATGATGGTTATG
PlACTIN	AAGGCTGGGTTTGCTGGAGATGAT	GACCACTGGCGTAGAGGGAAAGAA

样品编号	Raw read/bp	Raw base/bp	Clean read/bp	Clean base/bp	Q20碱基占比/%	Q30碱基占比/%	GC占比/%
CY_28d3	45 922 918	6 934 360 618	45 615 802	6 672 434 604	98.15	94.04	45.55
CY_28d2	42 048 084	6 349 260 684	41 698 894	6 093 148 642	97.99	93.70	46.06
CY_28d1	45 255 860	6 833 634 860	44 914 492	6 551 625 397	98.21	94.22	45.23
CY_CK3	43 119 016	6 510 971 416	42 868 668	6 407 178 267	98.04	93.78	46.02
CY_CK2	43 052 046	6 500 858 946	42 776 216	6 330 105 208	98.07	93.86	45.61
CY_CK1	43 060 196	6 502 089 596	42 743 518	6 358 480 584	98.00	93.69	45.84

样品编号	Raw read/bp	Raw base/bp	Clean read/bp	Clean base/bp	Q20碱基占比/%	Q30碱基占比/%	GC占比/%
CY_28d3	45 922 918	6 934 360 618	45 615 802	6 672 434 604	98.15	94.04	45.55
CY_28d2	42 048 084	6 349 260 684	41 698 894	6 093 148 642	97.99	93.70	46.06
CY_28d1	45 255 860	6 833 634 860	44 914 492	6 551 625 397	98.21	94.22	45.23
CY_CK3	43 119 016	6 510 971 416	42 868 668	6 407 178 267	98.04	93.78	46.02
CY_CK2	43 052 046	6 500 858 946	42 776 216	6 330 105 208	98.07	93.86	45.61
CY_CK1	43 060 196	6 502 089 596	42 743 518	6 358 480 584	98.00	93.69	45.84