浙江农业科学

浙江农业科学 ›› 2024, Vol. 65 ›› Issue (2): 325-328.DOI: 10.16178/j.issn.0528-9017.20221201

• 药用植物 • 上一篇    下一篇

激素和糖分对暗紫贝母生根培养的影响

字胡润1(), 赵艳娟2, 管世超1, 苏娟2, 文凤1, 张玉蓉1, 王晓2, 李靖瑞1, 岳健1,2,*()   

  1. 1.云南青谷生物科技有限公司,云南 昆明 650506
    2.云南山里红生物科技有限公司,云南 昆明 650229
  • 收稿日期:2023-02-15 出版日期:2024-02-11 发布日期:2024-02-28
  • 通讯作者: 岳健(1979—),男,云南昆明人,高级农艺师,硕士,从事中药材育种和种植技术研究工作,E-mail:yj79517@163.com。
  • 作者简介:字胡润(1992—),女,云南大理人,本科,从事植物组织培养研究工作,E-mail:ynqinggu@163.com。

Effects of hormones and sugar on rooting culture of Fritilaria unibracteata Hsiao et K.C.Hsia

Hurun ZI1(), Yanjuan ZHAO2, Shichao GUAN1, Juan SU2, Feng WEN1, Yurong ZHANG1, Xiao WANG2, Jingrui LI1, Jian YUE1,2,*()   

  1. 1. Yunnan Qinggu Biotechnology Co., Ltd., Kunming 650506, Yunnan
    2. Yunnan Shanlihong Biotechnology Co., Ltd., Kunming 650229, Yunnan
  • Received:2023-02-15 Online:2024-02-11 Published:2024-02-28
  • Contact: Jian YUE

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摘要：

本实验以苗龄为90 d的暗紫贝母芽繁苗为实验材料,在组培生根培养过程中添加不同浓度的激素和糖分,研究其对暗紫贝母生根的影响,筛选出暗紫贝母的最适生根培养基。结果表明:在生根培养基为基本培养基(MS)+9.4 g·L-1卡拉胶(car)+0.5 mL·L-1萘乙酸(NAA)+0.5 mL·L-1吲哚丁酸(IBA)+30 g·L-1蔗糖时暗紫贝母生根情况最好,生根率高达81.3%,根系粗壮,且有效根数最多,平均每株有3.4条根系,且没有被污染。本研究为以后建立暗紫贝母组培快繁体系、大规模标准化生产优良暗紫贝母种苗提供参考。

关键词: 暗紫贝母, 组织培养, 激素, 糖分, 生根

Abstract:

In this study, the 90-day-old buds of Fritilaria unibracteata Hsiao et K.C.Hsia were used as experimental materials, and different concentrations of hormones and sugars were added to study their effects on the rooting of Fritilaria unibracteata Hsiao et K.C.Hsia in tissue culture, and the most suitable rooting medium for Fritilaria unibracteata Hsiao et K.C.Hsia was screened. The results showed that the rooting medium was MS+9.4 g·L-1 car+0.5 mL·L-1 NAA+0.5 mL·L-1 IBA+30 g·L-1 sucrose had the best rooting effect, with a rooting rate of 81.3%, a thick root system, and the largest number of effective roots, with an average of 3.4 roots per plant, and no pollution. This study provides a reference for the establishment of a fast propagation system for tissue culture and large-scale standardized production of excellent Fritilaria unibracteata Hsiao et K.C.Hsia seedlings in the future.

Key words: Fritilaria unibracteata Hsiao et K.C.Hsia, tissue culture, hormones, sugar, rooting

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