Chemical residues affect the ecological balance of tea gardens and jeopardize the health of plants and animals. Biological agents developed by biotechnology, with high efficiency, low toxicity and specific typical characteristics, are widely used in the preparation of modern pesticides, fertilizers and bio-products active substances. Scienjpgic assessment of the safety and ecological application potential of biological agents is conducive to the optimization of the green production of intensive tea gardens and the promotion of high-quality development of the tea industry. Through reviewing relevant research literature at home and abroad, we analyzed the development history, functional role, main types and residue risk of chemical pesticides from the perspective of chemical replacement and environmental ecological protection in tea gardens, and explored the efficacy, application and potential of biological agents in green prevention and control of tea gardens, so as to provide support for the ecological application of biological agents in the green prevention and control of tea gardens. It was found that the overuse of chemical agents increased resistance of plant pathogens, jeopardized human and animal health, and caused serious impacts on the ecological environment, while biological agents originated from living organisms or their metabolites, and were ideally suited to inhibit fungi, bacteria, viruses, and nematode control in tea gardens. From the perspectives of product monitoring and biodiversity assessment, integrated prevention and control system and resistance management, and ecological risk assessment, it was proposed to establish safety control measures for biological agents. And from the perspectives of product quality enhancement, application timing and conditions, and application technology, it was proposed to improve the application efficiency of biological agents. Finally, from the perspectives of publicity and education, business model innovation, policy support and supervision, as was as research and development of related functional biomaterials and green inputs, it was proposed to strengthen the application and promotion of biological agents. Therefore, the application of biological agents instead of chemical agents is conducive to solving environmental risks and realizing green prevention and control.