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    11 April 2025, Volume 66 Issue 4 Previous Issue   

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    2025, 66 (4):  0. 
    Abstract ( 12 )   PDF (5866KB) ( 18 )  
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    Interpretation of local standard “Fertilization requirement of rice, wheat and rape in different regions” of Zhejing Province
    CHEN Yupei, SHAN Yingjie, LU Ruohui, ZHU Weifeng, KONG Haimin
    2025, 66 (4):  801-804.  DOI: 10.16178/j.issn.0528-9017.20231222
    Abstract ( 27 )   HTML ( 5 )   PDF (1076KB) ( 31 )  

    The formulation of chemical fertilizer application standard is the premise of promoting the chemical fertilizer reduction and utilization efficiency. After a full investigation and demonstration, the local standard “Fertilization requirement of rice, wheat and rape in different regions”of Zhejing Province was organized and formulated by Zhejiang Cultivated Land Quality and Fertilizer Management Station.The standard regulated the terms and definitions, division of fertilization area, principles of fertilizer application quantity determination, fertilization dosage, fertilization technology, fertilizer application management, etc. It provided for the comprehensive promotion of the chemical fertilizer reduction of rice, wheat and rape in Zhejiang Province.This paper interpreted the necessity of the standard formulation, the relevant technical content, and the determination basis of the specific indicators respectively, in order to increase the readability and operability of the standard.

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    Study on variability of separated generations in rice population breeding
    MO Junjie, WU Xiangyun, LIU Wentong, CHEN Churun, GAO Zhichao
    2025, 66 (4):  805-812.  DOI: 10.16178/j.issn.0528-9017.20240741
    Abstract ( 18 )   HTML ( 1 )   PDF (1171KB) ( 26 )  

    To investigate the genetic mechanism of multiple male paternal effect in rice population breeding, this study established a hybrid population using a two-line sterile line of rice, Hai S1, and four rice varieties. The main agronomic traits and coefficient of variation in different generations of the population were compared and analyzed. The results revealed that the variation coefficients for panicle number per plant and sterile plant rate in the separated generation population constructed using the rice population breeding were above 32%, indicating significant differences. The multiple male paternal hybrid exhibited more diverse variation types for key yield factors such as panicle number per plant, grain number per spike, and 1 000-grain weight compared with the single-male hybrid. This diversity wais beneficial for enriching genetic diversity related to rice yield traits and increasing opportunities for breeding selection. Sterile plants were isolated from different generations of the hybrid offspring constructed from light-thermo-sensitive sterile lines of rice. multiple male paternal rate of the hybrid offspring was over 10%, with the variation coefficient of the sterility rate being greater in the multiple male paternal hybrid offspring F3 than in the single paternal hybrid offspring F3. By utilizing male paternal hybrid progeny for multi-generation self-crossing and outcross mixing with continuously combining random mating populations and adding new parent germplasm at any time, it is possible to expand the genetic base. This approach leads to extremely rich and diverse isolated types among offspring which facilitates continuous innovation in rice germplasm resources.

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    Detection and analysis of resistance/susceptibility genes of rice blast and bacterial blight in 23 core rice materials
    ZHOU Hui, LIN Zhenpeng, ZHOU Haiping, WANG Chengbao, XU Xiuru, ZHONG Linping, MA Guohua
    2025, 66 (4):  813-818.  DOI: 10.16178/j.issn.0528-9017.20231140
    Abstract ( 16 )   HTML ( 0 )   PDF (1129KB) ( 17 )  

    Breeding disease-resistant varieties is the most effective approach to control rice diseases and pests. To efficiently assemble new disease-resistant combinations, molecular markers of 17 blast resistance genes and 6 bacterial blight resistance genes were used to detect resistance/susceptibility genes in 23 core rice materials. The blast resistance gene detection revealed that high-frequency resistance genes Pid2, Pi40 and Ptr were detected in 23, 15 and 23 materials respectively. Moderate-frequency genes Pi64, Pib, Pi2, Pigm, Pi5, Pi1 and Pikm occurred in 10, 10, 11, 10, 8, 10 and 8 materials respectively. Low-frequency genes Pit, Pi36, Pikh, Pia and Pita were only found in 2, 1, 3, 1 and 1 material. Piz and Pi25 were undetected. Conversely, susceptibility genes pi2, piz, pi25, pikh, pikm, pia and pita showed high frequencies (23, 23, 23, 15, 15, 22 and 22 materials respectively), while pi64, pigm, pi36 and pi5 exhibited moderate frequencies (9, 13, 13 and 13 materials). For bacterial blight genes, only resistance gene Xa27 was detected in 5 materials, while resistance genes xa5, Xa7, xa13, Xa21 and Xa23 were undetected. Susceptibility genes Xa5, xa7, Xa13, xa21, xa23 and xa27 showed high frequencies (23, 22, 23, 23, 23 and 14 materials respectively). This study systematically mapped the distribution of 23 reported resistance/susceptibility genes against rice blast and bacterial blight in 23 rice materials using molecular markers, providing valuable genetic references for subsequent screening, improvement and combination testing of rice germplasm.

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    Character analysis of rice varieties approved through different test channels in Jiangsu Province
    FAN Dejia, CHEN Shiqiang, HE Zhentian, ZHANG Rong, WANG Ruqin, CHENG Menghao, WANG Jianhua
    2025, 66 (4):  819-825.  DOI: 10.16178/j.issn.0528-9017.20240787
    Abstract ( 16 )   HTML ( 1 )   PDF (1501KB) ( 19 )  

    In recent years, the number of rice varieties approved in China has exploded, and the number of rice varieties approved in Jiangsu Province has also increased dramatically. In this study, the agronomic traits data of all rice varieties approved in Jiangsu Province from 2020 to 2024 were collected and classified according to indica-japonica subspecies and test channels. The differences in agronomic traits and annual trends of rice varieties approved in different test channels were compared and analyzed. From 2020 to 2024, there were 400 rice varieties approved in Jiangsu Province, including 73 indica rice and 327 japonica rice. There were 109 varieties participating in the provincial regional test, 228 in the combination test, 60 in the independent test, and 3 in the green channel. There were significant differences in some agronomic traits of rice varieties approved through different test channels. This study will help breeders to optimize breeding strategies and make the selected varieties more suitable for market demand.

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    Study on productivity of 4 conventional japonica rice varieties in northern Jiangxi Province
    LI Kun, LIU Zhonglai, CAO Guojun, PAN Shiwen, YU Xuan, ZHANG Yiyue, WANG Zhiquan
    2025, 66 (4):  826-829.  DOI: 10.16178/j.issn.0528-9017.20240011
    Abstract ( 11 )   HTML ( 0 )   PDF (1108KB) ( 12 )  

    In order to promote the strategy of “change from indica to japonica”in Jiangxi Province, meet the production demand of japonica rice in northern Jiangxi Province, and increase farmers' income and agricultural production efficiency, in this experiment, four conventional japonica rice varieties bred by the Rice Institute of Jiangxi Academy of Agricultural Sciences were selected, and Ganningjing 1 was selected as the control variety. Under the same field management conditions, the growth period, tillering, agronomic traits, yield components and yield were comprehensively compared. The results showed that 21Q251 had poor production performance and was not recommended to be popularized. 21Q216 showed the characteristics of strong tillering ability, high heading rate, high seed setting rate, good color transformation at ripening stage, strong disease resistance, and slightly low yield level. It is suggested that basic seedlings should be inserted enough and tillering fertilizer should be applied early to ensure sufficient effective panicle. Ganwanjing 688 has good high yield and stable yield performance, showing strong tillering ability, high heading rate, high seed setting rate, good color transformation at ripening stage, strong lodging resistance and disease resistance, so it is recommended to be popularized in northern Jiangxi Province.

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    Breeding of new indica-japonica hybrid rice combination Zhejingjiayou 710 with high yield and good quality
    ZHANG Xinyue, YE Shenghai, DONG Junjie, FU Haowei, LI Youfa
    2025, 66 (4):  830-833.  DOI: 10.16178/j.issnv0528-9017.20240921
    Abstract ( 13 )   HTML ( 0 )   PDF (1106KB) ( 20 )  

    Zhejingjiayou 710, derived from japonica-type CMS line Zhejing 7A and indica-type restorer line DR610, is a new indica-japonica hybrid rice combination co-developed by Jiaxing Academy of Agricultural Sciences, Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences and Zhejiang Wuwangnong Seed Industry Co., Ltd. With the characteristics of high yield, wide adaptability and good grain quality, it was registered and released by the National Crop Variety Approval Committee in 2022. The breeding process, characteristics and key technical points in cultivation and hybrid seed production of Zhejingjiayou 710 were introduced in this study.

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    Breeding of a new high-quality and disease-resistant three-line hybrid japonica rice variety Jiayou 10
    ZHANG Chengxiang, LI Bai, LU Jingen, GAO Rongcun
    2025, 66 (4):  834-837.  DOI: 10.16178/j.issn.0528-9017.20240503
    Abstract ( 13 )   HTML ( 0 )   PDF (1107KB) ( 13 )  

    Jiayou 10 is a new three-line hybrid japonica rice variety developed by crossing the BT-type early-maturing medium japonica male sterile line Jia 57A with the restorer line Jiahui 10. This variety exhibits excellent comprehensive disease resistance, superior grain quality, and high seed production yield. It was approved by the Shanghai Municipal Crop Variety Approval Committee in 2022. This paper analyzed the agronomic traits, yield performance, grain quality, and disease resistance of Jiayou 10. Additionally, the key cultivation techniques and seed production techniques for this variety were summarized, providing a theoretical basis for its popularization and application.

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    Breeding, characteristics and cultivation techniques of a new high yield and long-grain indica and japonica hybrid rice variety Zheyou 77
    QI Yongbin, WANG Linyou, LYU Xiangchao, JIANG Genshui, ZHANG Haodi
    2025, 66 (4):  838-842.  DOI: 10.16178/j.issn.0528-9017.20240575
    Abstract ( 12 )   HTML ( 2 )   PDF (1111KB) ( 17 )  

    Zheyou 77, a new combination of long-grain indica and japonica hybrid rice, is a medium-maturity three-line indica and japonica hybrid rice co-bred by Crop and Nuclear Technology Utilization Institute of Zhejiang Academy of Agricultural Sciences, Guzhiyu (Huzhou, Zhejiang) Agricultural Technology Co., Ltd., and Hangzhou Seed Industry Group. It was composed of long-grain japonic-based sterile line Zhehang K68A (Zhe75A) as the maternal parent and a wide-compatibility partial indica restorer line Zhehui F27 as the male parent, and passed the Zhejiang provincial variety approval (Zhejiang-approved Rice 2024017) in 2024. Zheyou 77 has the characteristics of high yield, excellent rice quality, good color transformation and wide adaptability, which is suitable for single season late rice planting in Zhejiang Province and surrounding rice areas. In this paper, the breeding, variety characteristics and cultivation techniques of Zheyou 77 were briefly introduced.)

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    Breeding and application of round grain specialized two-line early indica rice restorer line Fu 269
    WANG Wei, QI Yongbin, WANG Junmin, XIAO Changming, LI Kekuan, LIU Chaogang, LIU Qinglong
    2025, 66 (4):  843-847.  DOI: 10.16178/j.issn.0528-9017.20240308
    Abstract ( 10 )   HTML ( 0 )   PDF (1117KB) ( 15 )  

    Fu 269, a two-line early indica rice restorer line, was developed through the pedigree breeding method by irradiating F1 dry seeds of Zhong 07-108 × 04YK17 using 80 Gy γ-rays. It has the primary characteristics of strong restoring ability, heavy pollen load, high hybrid seed yield, round grain, high amylase content, high resistance to rice blast, et al. The two-line early indica hybrid rice combination Lingliangyou 69 (the combination of Xiangling 628S×Fu 269) was approved by the Jiangxi Provincial Crop Variety Approval Committee in 2018 and registered for introduction in Hunan Province in 2019.

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    Selection experiment of high-yield regenerated rice varieties suitable for Changsha Region
    QIAN Yanjie, LI Cheng, YAO Yanhong, HU Mingyong, WANG Wei, JIANG Yu, LUO Wei, XIAO Feifei, WANG Shaoxi
    2025, 66 (4):  848-853.  DOI: 10.16178/j.issn.0528-9017.20240414
    Abstract ( 10 )   HTML ( 1 )   PDF (1099KB) ( 12 )  

    In order to select suitable varieties of regenerated rice for planting in Changsha Region, 16 hybrid rice varieties (combinations) that have been approved in recent years were used as materials,and Y Liangyou 911 (CK) was used as the control to conduct adaptability experiments on regenerated rice yield, growth period, and other important traits. Scientific evaluation was conducted based on plant type, growth period, yield and yield composition, and other important performance indicators. The results showed that the top three varieties in terms of annual yield among the tested varieties were Gongliangyou Yue Nongsi Miao, Hengfeng You Yue Nongsi Miao, and Yangtaiyou 5009, with an average yield of 853.9-869.9 kg per 667 m2, which wais 7.49%-9.50% higher than Y Liangyou 911 (CK). Gongliangyou Yue Nongsi Miao, Hengfengyou Yue Nongsi Miao, and Yangtaiyou 5009 have excellent comprehensive characteristics, which are more suitable for promotion and planting in the Changsha Region.

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    Effects of nitrogen fertilizer reduction combined with foliar silicon fertilizer on agronomic traits and yield of Huazheyou 261
    TAO Ruofu, XIA Ruda
    2025, 66 (4):  854-857.  DOI: 10.16178/j.issn.0528-9017.20240289
    Abstract ( 14 )   HTML ( 0 )   PDF (1453KB) ( 17 )  

    To explore the effect of different nitrogen fertilizer reduction combined with foliar silicon fertilizer on the yield of continuous cropping late rice Huazheyou 261, three different nitrogen fertilizer application rates (N1, pure nitrogen 180 kg·hm-2, N2, pure nitrogen 162 kg·hm-2, N3, pure nitrogen 144 kg·hm-2) and two different silicon fertilizer application rates (S1, foliar spraying with water, S2, foliar spraying with silicon fertilizer 3 000 mL·hm-2) were designed, and the effects of different nitrogen fertilizer and silicon fertilizer application on the agronomic traits and yield were studied according to the split-plot experimental design. The results showed that under the treatment of nitrogen fertilizer reduction, the grains per panicle, filled grain per panicle and 1 000-grain weight of Huazheyou 261 decreased, and the yield decreased. Compared with the conventional high-yield cultivation mode (N1S1), under the treatment of reducing nitrogen by 10% (N2S1), the yield of Huazheyou 261 decreased by 2.18%; under the treatment of reducing nitrogen by 20% (N3S1), the yield of Huazheyou 261 decreased by 4.21%. The application of foliar silicon fertilizer can increase the yield of Huazheyou 261 and make up the negative effect of nitrogen fertilizer reduction on yield.

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    Effects of different new fertilizer application on yield and quality of rice
    LIU Guoqun, ZHU Chunquan, LIU Xiaoxia, LI Ronghui, YAN Wenting, SUN Xiuhua, SHANG Xiaolan, ZHU Lianfeng, TIAN Wenhao, ZHANG Junhua
    2025, 66 (4):  858-862.  DOI: 10.16178/j.issn.0528-9017.20240187
    Abstract ( 18 )   HTML ( 1 )   PDF (1206KB) ( 26 )  

    The high-yielding hybrid rice cultivars of Yongyou 1540 was adopted as the test material in this study. Two types of fertilizers, resin-coated slow-release fertilizer (Maoshi) and stabilized fertilizer (Shikefeng), were selected. Three different nitrogen fertilizer allocation ratios were set. The results showed that compared with the conventional fertilization, the 667 m2 yield of rice was increased by 21.91%, reaching 527.63 kg, when the slow-release fertilizer (Maoshi) was applied with a ratio of 5:2:3 for base fertilizer, tillering fertilizer, and panicle fertilizer, and it did not affect the grain quality. Compared with the conventional fertilization, applying stabilized fertilizer (Shikefeng) with a ratio of 5:2:3 for base fertilizer, tillering fertilizer, and panicle fertilizer increased rice yield and nitrogen use efficiency without affecting grain quality. The results indicate that optimizing the appication of slow-release fertilizer was beneficial to improve soil fertility, promote nitrogen utilization, and significantly increase rice yield by increasing the number of panicles.

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    Analysis of high yield and stable yield of silage maize in Huanghuaihai Region based on GGE biplot
    LI Shufen, CHAI Wenbo, XU Hanyuan, ZHU Qing, LI Hongtao, YUAN Chao, WANG Jun
    2025, 66 (4):  863-868.  DOI: 10.16178/j.issn.0528-9017.20240341
    Abstract ( 11 )   HTML ( 1 )   PDF (4089KB) ( 11 )  

    In order to determine the high yield and stable yield of the trial varieties of summer sown silage maize in Huanghuaihai Region in 2020, and to provide reasonable analysis methods and theoretical references for further variety promotion and planting, data from 15 varieties (lines) and 13 pilot sites of the 5 000 seedlings per 667 m2 density group regional trial of summer sown silage maize in the Huanghuaihai Region in 2020 were selected. The analysis method of genotype main effects and genotype×environment interaction (GGE) biplot was applied to analyze the high yield and stable yield of the trial varieties, as well as the differentiation and representativeness of the pilot sites. The results showed that the varieties with high yield and stable yield were Wannongke Silage 6, Chuandan 99, Lian Silage 101, and Yuqingyu 9, which were also the ideal varieties in this group of regional trials. The ideal variety is suitable for planting and promotion in most areas of Huanghuaihai Region. Shijiazhuang City, Hebei Province is the most ideal pilot for this regional trial.

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    Breeding of a new high-quality black sweet corn variety Zheheitian 208
    WANG Tingzhen, CHEN Jianjian, WU Zhenxing, LI Fangjian, LYU Guihua
    2025, 66 (4):  869-873.  DOI: 10.16178/j.issn.0528-9017.20240781
    Abstract ( 11 )   HTML ( 0 )   PDF (3679KB) ( 11 )  

    Zheheitian 208 is a new high-quality black fruit type sweet corn variety, which was bred by crossbreeding 18hi224 as the female parent and 19hiRh367 as the male parent at the Institute of Corn and Characteristic Dry Grains, Zhejiang Academy of Agricultural Sciences. This variety has black seeds, high yield, high sweetness, strong adaptability, and good appearance. It passed the variety approval of Guangxi Zhuang Autonomous Region in 2024 (Guishenyu 2024016). This article introduced the breeding process and variety characteristics of Zheheitian 208, summarized the key points of supporting cultivation techniques and seed production techniques, and provided reference for promoting the planting of Zheheitian 208.

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    Screening test of high-quality fresh waxy corn varieties in Zhaoqing City
    LIANG Mingran, DENG Qiwen, YAN Xiancheng, HUANG Dongrui, LIN Xiaonan, WANG Gangfeng, MAI Jiaqi
    2025, 66 (4):  874-878.  DOI: 10.16178/j.issn.0528-9017.20240559
    Abstract ( 9 )   HTML ( 1 )   PDF (1102KB) ( 12 )  

    In order to strengthen the promotion of new varieties of fresh waxy corn and select new varieties with high yield, high quality and good adaptability that are suitable for popularization and planting in Zhaoqing City, the main agronomic traits, yield and taste quality of 9 fresh waxy corn varieties were compared in Shapu Base of Zhaoqing Agricultural Improved Varieties Demonstration and Extension Center in 2023. The results showed that Ruiyu 926 waxy corn, Guangliang Bainuo No.7, Guangliang Cainuo No.6 and Tianwei Nuo No.9 had good comprehensive performance, and could be used as ideal varieties of high yield and high quality fresh waxy corn in Zhaoqing City.

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    Evaluation of disease resistance and high yield of different fresh waxy maize varieties
    CHEN Yixiang, ZHANG Xiaomei, GOU Yuhong, XIA Zaixing, GUO Tao, ZHANG Bisheng
    2025, 66 (4):  879-884.  DOI: 10.16178/j.issn.0528-9017.20240733
    Abstract ( 10 )   HTML ( 0 )   PDF (1107KB) ( 12 )  

    To select high-quality fresh waxy maize varieties suitable for planting in Mangshi of Dehong Prefecture of Yunnan Province, the new varieties of fresh waxy maize Decainuo, Ruinuo 8, Yunnuo 9, Sidanuo 68, Sidanuo 70 and Tianminuo 29 were selected to conduct field experiment through the comprehensive evaluation of growth period, agronomic traits, disease resistance, fresh ear yield, quality scores, and other indicators in comparison of main cultivation variety Shinuo 2 (control) in the experimental base of Dehong Agricultural Science Research Institute in 2023. The results showed that fresh ear yield (10 528.30-14 834.08 kg·hm-2) of tested varieties were higher than that of control, and the total score of sensory quality and cooking quality was 78-88. All tested varieties did not occur leaf spot disease, small spot disease and grey spot disease in the field, but there were two new varieties (Sidanuo 70, Tianminuo 29) occurred rust disease, while other varieties did not occur rust disease. Compared with other varieties, the new varieties Decainuo and Yunnuo 9 showed moderate growth period, semi-compact plant type, long conical ear shape, more grains number, high grain-weight, good field uniformity, stable agronomic traits, strong disease resistance, obvious high yield ability, excellent cooking quality, which are suitable for further test and promotion in Mangshi Area.

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    Effects of humic acids on the growth and drought resistance of wheat seedlings
    ZHANG Yunhong, REN Ziwen, MAO Jiawei, YANG Yonghui, GAO Cuimin, HAN Weifeng, LIU Xiaoqi
    2025, 66 (4):  885-892.  DOI: 10.16178/j.issn.0528-9017.20240312
    Abstract ( 10 )   HTML ( 1 )   PDF (1183KB) ( 12 )  

    This study employed a hydroponic method to investigate the effects of different humic acid applications on wheat seedling growth and drought resistance under polyethylene glycol (PEG)-simulated drought conditions. Principal component analysis (PCA) was used to construct a comprehensive evaluation index for wheat seedlings to assess the effectiveness of humic acid in promoting growth and enhancing drought resistance, aiming to screen high-activity humic acid products. The results showed that under normal growth conditions, the F8, F4, and F5 treatments achieved the highest comprehensive scores and better growth performance. Specifically, the fresh weight of the underground parts of wheat seedlings treated with F8 was significantly higher than that of CK, with an increase of 24.3%. The chlorophyll b content in the F5 and F8 treatments also increased significantly. Under drought stress, the seedling length and underground fresh weight of wheat treated with F3 were significantly higher than those of CK2, with increases of 15.4% and 10.3%, respectively. The underground fresh weight and catalase (CAT) activity of wheat treated with F4 increased significantly by 12.5% and 4.1%, respectively, compared with CK2. In contrast, the proline content in the F5 treatment decreased significantly by 72.6% compared with CK2. PCA results indicated that F8, F4, and F5 treatments were more effective in promoting wheat growth, while F3, F4, and F5 treatments performed better in alleviating drought stress. Under the experimental conditions, among the 10 humic acids tested, F4 and F5 demonstrated the best application effects.

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    Comparison experiment of high-quality and high-yield wheat varieties in Deqing County
    REN Jie, MI Sheng, LIN Qin
    2025, 66 (4):  893-896.  DOI: 10.16178/j.issn.0528-9017.20240304
    Abstract ( 10 )   HTML ( 2 )   PDF (1079KB) ( 15 )  

    In recent years, wheat cultivation in Deqing County has showed a sharply increased trend, with the planting area continuously expanding, and has become an important growth point and breakthrough point for stabilizing grain supply in Deqing County. To further discover the potential of winter wheat cultivation, the experiment was conducted using 11 introduced wheat varieties as materials, aiming to screen out high-quality and high-yield wheat varieties suitable for planting in Deqing County. The results showed that spring wheat Yangmai 23, Yangmai 28, Zhehua No.1, and Ningmai 22 had outstanding advantages in high yield and lodging resistance, with good comprehensive performance, which were suitable for further demonstration and promotion in Deqing county.

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    Screening and demonstration of semi-wintering wheat varieties in Jiangsu region along Huaihe River
    XU Mengbin, ZHOU Xing
    2025, 66 (4):  897-903.  DOI: 10.16178/j.issn.0528-9017.20240840
    Abstract ( 10 )   HTML ( 0 )   PDF (1104KB) ( 12 )  

    In order to screen semi-wintering wheat varieties suitable for planting in Jiangsu region along Huaihe River, a variety screening test was carried out in Huaihai Farm, Linhai Farm and Xinyang Farm in Jiangsu region along Huaihe River in 2022-2023, selecting 19 semi-wintering wheat varieties validated in recent years with greater potential for popularization, and using Huaimai 33 as the control variety, and carrying out a comprehensive screening and identyficatin of their field growth, yield performance and resistance. The results showed that the average yield level of the control Huaimai 33 was higher and the average yield per 667 m2 was 742.6 kg, and the average yields of Zhengmai 1860 and Yannong 1212 varieties were higher than that of the control Huaimai 33, in which the average yield of Zhengmai 1860 increased by 1.0%, and that of Yannong 1212 increased by 0.3% compared with the control. In addition, Zhengmai 1860 and Yannong 1212 varieties have better performance in the three pilots, and are more widely adapted. In summary, Zhengmai 1860 and Yannong 1212 can be widely planted in Jiangsu region along Huaihe River.

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    Accumulation regularity of isoflavone and its components during soybean seed development
    YU Xiaomin, YUAN Fengjie, FU Xujun, YANG Qinghua, JIN Hangxia, ZHU Longming
    2025, 66 (4):  904-909.  DOI: 10.16178/j.issn.0528-9017.20240464
    Abstract ( 12 )   HTML ( 2 )   PDF (1138KB) ( 15 )  

    Isoflavones, also known as phytoestrogens, are a class of secondary metabolites mostly produced in soybean seeds. This study focused on the differences about total isoflavone content and 12 isoflavone components during seed development among five soybean varieties using high performance liquid chromatography. During the progressing of seed development, the content of isoflavones continued to increase in the seeds of the five soybean varieties. However, there were significant differences in the accumulation mode and rate among the varieties. The proportion of the main components such as malonyl genistein, malonyl daidzein, genistein and daidzein changed among the five varieties according to the total isoflavone content. The accumulation pattern of genistein and daidzein (two physiological active components) were also different compared with the main components. The breeding and production of high-quality soybean varieties with high isoflavone content is supported by studying the physiological mechanism of isoflavone accumulation in soybean seeds.

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    Demonstration and extension of soybean-maize strip compound planting in Anhui Province
    PAN Guangyuan, YANG Sen, LI Xue, ZHANG Jun, ZHAI Pengfei, KONG Lingjuan
    2025, 66 (4):  910-916.  DOI: 10.16178/j.issn.0528-9017.20240135
    Abstract ( 9 )   HTML ( 1 )   PDF (2165KB) ( 13 )  

    In order to fully understand the demonstration and extension of soybean-maize strip compound planting in Anhui Province, study and solve the problems existing in technology, policy and machinery, summarize work experience, put forward countermeasures and suggestions, and provide theoretical and scientific basis for large-scale promotion of this technology,new agricultural management entities and small farmers were investigated in the main producing areas through questionnaires and on-site visits. This paper systematically expounded the background and significance of planting, deeply analyzed the current situation of planting development, refined the enlightenment of working practices, summarized the main existing problems, and put forward countermeasures and suggestions.

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    Introduction practice of olive varieties Arbequina and Nichiskii Ⅰ in the South of China
    NIU Erli, LI Xue, GAO Song, WANG Wei, FU Yulou, ZHU Shenlong
    2025, 66 (4):  917-922.  DOI: 10.16178/j.issn.0528-9017.20241001
    Abstract ( 10 )   HTML ( 0 )   PDF (4974KB) ( 13 )  

    To screen superior olive cultivars suitable for cultivation in southern China and further guide the development of the olive industry, this study conducted a comprehensive analysis and tracking investigation of two olive cultivars, Arbequina and Nichiskii Ⅰ, planted in 2018. The results showed that both cultivars began flowering and fruiting two years after planting. After six years, their tree heights reached 3.21 and 3.34 m, with tree diameters of 8.19 and 8.39 cm, respectively. Arbequina produced round fruits with an average single fruit weight of 1.34 g. Its fresh fruit yield per 667 m2 was 654.85 kg, yielding 68.76 kg of olive oil, with oil extraction and oil content rates of 10.59% and 14.90%, respectively. Nichiskii Ⅰ produced olive fruits with an average single fruit weight of 4.24 g. Its fresh fruit yield per 667 m2 was 1 050.00 kg, yielding 81.90 kg of olive oil, with oil extraction and oil content rates of 7.89% and 14.00%, respectively. The peroxide values of olive oil from both cultivars were 0.067%, while acid values were 1.20 and 0.11 mg·g-1 for Arbequina and Nichiskii Ⅰ.The ultraviolet absorbance values D232,D270,and ΔD270 of the olive oil from the Douguo variety are 1.871 1, 0.207 6, and 0.001, respectively. For the Ni I variety, the ultraviolet absorbance values D232,D270,and ΔD270 are 1.934 0, 0.202 0, and 0.003, respectively. The quality of oil from both varieties meets the, respectively, meeting the quality standards for extra virgin olive oil. The unsaturated fatty acid content accounted for 76.19% and 78.18% of total fatty acids in Arbequina and Nichiskii Ⅰ, respectively. These findings indicate that both cultivars demonstrate strong adaptability to mountainous regions in southern China, with excellent oil yield and quality, highlighting their significant application prospects.

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    Study of atractylolides determination in Atractylodes lancea (Thunb.) DC.by UPLC-MS/MS
    ZHANG Wenwen, WU Qinyan, ZHAO Laicheng, LIANG Hongfang, WANG Jianhua, CHEN Lu
    2025, 66 (4):  923-927.  DOI: 10.16178/j.issn.0528-9017.20231167
    Abstract ( 9 )   HTML ( 0 )   PDF (1392KB) ( 13 )  

    A method for determination of atractylolide Ⅰ, atractylolide Ⅱ and atractylolide Ⅲ in Atractylodes lancea (Thunb.) DC. by ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated. Ion pairs with high ionization effect were obtained via feature scan of mass spectrometry. The samples were extracted with methanol at room temperature, the liquid-solid ratio was 20 mL·g-1, and the ultrasonic extraction time was 45 min. The extraction amount of the three atractylolides reached a high level, which were 621.33、201.32 and 275.97 μg·g-1 respectively; The recoveries of the three atractylodes were in the range of 85.7%-106.2% with the relative standard deviations of 6.0%-9.7%. The detection limit and quantification limit of the method are 18.2-32.2 μg·L-1 and 61.0-111.2 μg·L-1, respectively. This method can be suitable for the simultaneous analysis of three atractylodes in Atractylodes lancea.

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    Preliminary study of a non-insect lower animal from Fritillaria thunbergi
    WU Jun, XIE Yunye, FANG Li, ZONG Kankan, SHAN Binkai, WANG Pan, CHEN Shoufu, WANG Lianping, WANG Hanrong
    2025, 66 (4):  928-931.  DOI: 10.16178/j.issn.0528-9017.20231189
    Abstract ( 9 )   HTML ( 0 )   PDF (13392KB) ( 12 )  

    Fritillaria thunbergii is a most famous of “Zhejiang eight taste” medical plants, and it has been become a major cash crop. From 2016 to 2022, we observed that the growth of Fritillaria thunbergii plant was very poor, the root of Fritillaria thunbergii was very weakly, these plants were gradually withered and died. The suspected diseased plants were collected and transpoted to laboratory for further analysis. Based on bulb of Fritillaria thunbergii feeding and morphological observation it was preliminarily identified that Onychiuyus fimeitayius infected Fritillaria thunbergii. The study can provide a certain basis for the green prevention and control.

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    Comparative trial of spring vegetable-soybean varieties
    JIN Shuifeng, ZHANG Dan, WU Jianlie, HUANG Yue, ZHONG Yuting, RUAN Qianqian, ZHANG Yuwei, ZOU Yijing
    2025, 66 (4):  932-934.  DOI: 10.16178/j.issn.0528-9017.20240609
    Abstract ( 9 )   HTML ( 0 )   PDF (1065KB) ( 14 )  

    A comparative trial was conducted on 13 spring vegetable-soybean varieties, evaluating indicators such as growth period, plant traits, pod traits, and yield. It was found that Zhexian 12 and Zhenong 17 could be sown earlier to enhance planting efficiency. Zhenong 6 and Zhexian 9 are suitable for continued promotion and application. Sucheng 6 and Zhenong 11, with their strong plant growth vigor, high yield, green pods, plump grains, and good marketability, are highly favored by large-scale growers and have promising prospects for promotion and application. Four varieties, Kaikeyuan Lufeicui, Sucheng 2, Xuchun 4, and Kaikeyuan Cuilyubao, showed good overall performance and are recommended for multi-site trials to facilitate their promotion.

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    Study on the alleviating effect of different application methods of biochar on continuous cropping obstacles in broad bean
    LIU Shuxin, WU Dongtao, LI Hanmei, DING Fenghua
    2025, 66 (4):  935-939.  DOI: 10.16178/j.issn.0528-9017.20231231
    Abstract ( 11 )   HTML ( 1 )   PDF (2077KB) ( 16 )  

    In this study, four different application methods of biochar were designed, the soil with severe continuous cropping obstacle of broad bean was used as the test material, the effects of different application methods of biochar on the degradation of phenolic acids, microflora, enzyme activity, yield and quality of broad bean in rhizosphere soil were investigated. The results showed that the different application methods of biochar could reduce the content of phenolic acids in rhizosphere soil, improve soil enzyme activity and change the microflora in rhizosphere soil of broad bean. Under the four application methods, the effect of biochar was different. Based on the comprehensive analysis of soil environment and yield and quality indicators of broad bean, the deep application of row replacement was better than the shallow application of row replacement, the deep application of the planting hole was better than the spreading application of the planting hole, and the deep application of row replacement was better than the deep application of the planting hole.

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    Effects of different mycelium cultivation methods on mycelial growth and yield of Pleurotus geesteranus
    LANG Qiangjun, WANG Song, SONG Jiling
    2025, 66 (4):  940-944.  DOI: 10.16178/j.issn.0528-9017.20240736
    Abstract ( 8 )   HTML ( 0 )   PDF (3585KB) ( 12 )  

    In order to alleviate the effects of summer facility cultivation of Pleurotus geesteranus due to factors such as insufficient cultivation sites and cultivation techniques, the research on the key technology of centralized mycelium cultivation with different cultivation methods(liquid and solid) for Pleurotus geesteranus was carried out. Taking Tae Sau as the test material, the study was conducted by transferring the mushrooms to the greenhouse after cultivating them for a period of time at constant temperature (20-22 ℃). Through comprehensive analysis of the indicators including mycelial growth, time of anlage occurrence, contamination, mushroom production and yield, it was determined that liquid cultivation at constant temperature for 30 d and solid cultivation for 25 d had little effect on the yield and mushroom production, and it was suitable to be applied in the actual growth. It was also found that the environmental conditions should be strictly controlled in the stage to cultivate the bacterial bag and avoid excessive environmental stimulation, which would affect the mushroom production and yield.

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    Test and research on micro bud grafting technology of Ougan (Citrus suavissima Hort. ex Tanaka)
    WANG Hong, HONG Fanglei, ZHENG Bosheng, YU Hongao
    2025, 66 (4):  945-951.  DOI: 10.16178/j.issn.0528-9017.20241004
    Abstract ( 7 )   HTML ( 0 )   PDF (5886KB) ( 17 )  

    The experiment primarily focused on obtaining sterile rootstocks and scions for citrus micro-grafting, and investigated the effects of shoot tip size and grafting methods on the survival rate of in vitro micro-grafting in Ougan citrus. Additionally, the effectiveness of Huanglongbing (HLB) pathogen elimination in grafted seedlings was detected using RT-PCR technology. The results showed that local Zhuluan seeds and Guangxi trifoliate orange seeds exhibited the highest germination rate in the current year. Using Guangxi trifoliate orange seeds as rootstocks, the optimal germination conditions were achieved by disinfecting the seeds with 50% Bijieshi disinfectant for 10-20 minutes and culturing them in a water + agar medium, yielding a sterile germination rate of 43.5%. The survival rates of micro-grafting varied significantly among different rootstock varieties. Among the four rootstock varieties tested, the ease of micro-grafting survival followed the order: Zhuluan>trifoliate orange>fragrant orange>red tangerine. Triangular bud grafting was performed using a stem tip diameter of 0.2-0.4 mm. The survival rate of the stem tip was 42.5% after 30 days grafting. The removal rate of Huanglongbing was 41.6%.

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    Effects of plant growth regulators on the fruit flavor quality of Shine Muscat grape
    CHEN Zhe, XU Chao, LI Xiaoting, HUANG Yali, ZHU Zuoyi, DAI Fen
    2025, 66 (4):  952-957.  DOI: 10.16178/j.issn.0528-9017.20231139
    Abstract ( 8 )   HTML ( 0 )   PDF (1262KB) ( 13 )  

    In order to investigate the effects of different concentrations and combinations of plant growth regulators such as gibberellin (GA3), forchlorfenuron (CPPU), and thiobenzene (TDZ) on the fruit quality of Shine Muscat grapes, this study used 3-year-old Shine Muscat grapes as experimental materials. The results showed that different treatments had little effect on the content of soluble total sugar and soluble solids in fruits. However, under the same concentration of GA3 , the fruit treated with TDZ + GA3 had a higher solid acid ratio compared with the fruit treated with CPPU + GA3, apart from that, there was no significant change in the total phenolic and vitamin C content. Similarily, under the same concentration of GA3 of treatment, the fruit treated with TDZ + GA3 had a higher content of alcohols, while the content of terpenes in the fruit treated with CPPU+ GA3 was relatively higher. In addition, the content of aroma components with fragrant and fruity characteristics such as hexanal and 1-hexanol increased in the fruits treated with TDZ. Based on comprehensive comparative analysis, 2 mg·L-1 GA3 was used to pull the flower bud 15 days before flowering, 20 mg·L-1 GA3+2 mg·L-1 TDZ was used to dip the inflorescence 2-3 days after full flowering, and 20 mg·L-1 GA3+2 mg·L-1 TDZ was used to dip the fruit clusters 12-15 days after full flowering to obtain the best flavor of the Shine Muscat grape fruit.

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    Study on the technology for adjusting the color of cut flowers of hydrangea to blue
    SUN Xiaobo, CHEN Shuangshuang, CHEN Huijie, FENG Jing, QI Xiangyu, DENG Yanming
    2025, 66 (4):  958-963.  DOI: 10.16178/j.issn.0528-9017.20231202
    Abstract ( 9 )   HTML ( 1 )   PDF (4184KB) ( 14 )  

    This study explored the effect of preservation solutions containing different types of aluminum salts on adjusting red (pink or reddish-pink) cut flowers of hydrangea to blue, with the aim of identifying suitable aluminum salts and their optimal concentrations to achieve both color transition and acceptable vase life. Using Kelixian professional 2 preservative as a control, the vase life and blueing time of red (pink or reddish-pink) cut flowers of hydrangea were observed under treatment with Kelixian professional 2 preservative containing different types of aluminum salts and concentrations of aluminum citrate. The aluminum ion content in flower branches and sepals of cut flowers of hydrangea under different aluminum citrate treatments was also measured. Additionally, the effects of different branch lengths and leaf numbers on the vase life and blueing time of cut flowers of hydrangea were studied. The results showed that preservation solutions containing aluminum citrate made red (pink or reddish-pink) cut flowers of hydrangea turn blue during the vase period. The longer the flower branches and the more leaves there were, the longer the time for the sepals to turn blue, while the vase life of cut flowers of hydrangea was significantly shortened. Higher concentrations of aluminum citrate (0.010 0 mol·L-1) can significantly shorten the blueing time for cut flowers of hydrangea and also significantly shorten the vase life; The preservation solutions containing lower concentrations of aluminum citrate (0.002 5 and 0.005 0 mol·L-1) relatively prolonged the blueing time and vase life of cut flowers of hydrangea, and can be used as a working concentration for making red (pink or reddish-pink) cut flowers of hydrangea turn blue. The higher the concentration of aluminum citrate in the preservation solutions was, the faster the cut flowers absorbed aluminum ions, and the more aluminum ions were transported and stored in the sepals.

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    Morphological characteristics in hybrid generation of crosses between azalea varieties such as Carnation and Chinese native Yingshanhong
    LIAO Wenhui, SHAO Changsheng, GONG Zhongxing, ZHENG Huijun
    2025, 66 (4):  964-968.  DOI: 10.16178/j.issn.0528-9017.20240986
    Abstract ( 7 )   HTML ( 0 )   PDF (2335KB) ( 11 )  

    The crossbreeding research was carried out on azalea using the multi-seasonal flowering azaleas varieties such as Carnation, Pink Ribbons and Chiffon as female parent and the Chinese native Yingshanhong (CHN1/2/3) as male parent. Statistical analyses were performed on seed quality of single capsule and seed germination rate of hybrid females, and flower type and flower color of the first hybrid generation. The results showed that Carnation had the highest affinity in crosses with CHN1/2/3, followed by Chiffon, and Pink Ribbons was the lowest; Flower colors and flower types of the first hybrid generation showed significant trait segregation, and the flower types showed dominant inheritance. The results of this study provide a good reference for the breeding of new azalea varieties.

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    GC-MS analysis of volatile oil components in flower buds of four Magnolia plant species
    WANG Junfeng, WU Weijian, LIU Xi, TANG Ziyi, JIANG Ming
    2025, 66 (4):  969-972.  DOI: 10.16178/j.issn.0528-9017.20240007
    Abstract ( 8 )   HTML ( 0 )   PDF (1198KB) ( 12 )  

    Dried flower buds of M. soulangeana, M. amoena, M. biondii, and M. denudata were used as materials to extract their volatile oil components using the steam distillation method. GC-MS was applied to determine and compare their constituents. The results revealed that there were a total of 140 substances in the volatile oils of the four Magnolia species, with M. soulangeana having the most of 86 and M. amoena having the least of only 63. Terpenoids were the most abundant substances, with 48, 33, 42, and 40 types in M. soulangeana, M. amoena, M. biondii, and M. denudata, respectively. Totally 27 substances were common to all four species, including alcohols such as eucalyptol, β-terpineol, and δ-pinanol, as well as terpenoids such as β-caryophyllene, cadinene, and myristicin. This study provides a foundation for the development and utilization of medicinal ingredients from Magnolia species in the future.

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    Different water and fertilizer treatments on the ornamental traits and root activity of Phalaenopsis aphrodite
    TANG Xiaowei, ZHANG Ningning, LIU Chen, HENG Yan, QU Xiaohui, SHAO Heping
    2025, 66 (4):  973-978.  DOI: 10.16178/j.issn.0528-9017.20231227
    Abstract ( 8 )   HTML ( 0 )   PDF (1292KB) ( 13 )  

    In order to explore the effects of different water and fertilizer treatments on the ornamental quality of Phalaenopsis aphrodite, this study used Sanseniao as the experimental variety and applied water-soluble fertilizers commonly used in Phalaenopsis aphrodite production. Five different water and fertilizer treatment combinations were set up to screen for the optimal fertilization method that promotes the flowering quality and root vitality of Phalaenopsis aphrodite. The results showed that there was varying degrees of correlation between the 12 ornamental traits of Phalaenopsis aphrodite. Through principal component analysis, three principal components were extracted with contribution rates of 52.590%, 28.364%, and 11.071%, respectively, with a cumulative contribution rate of 92.025%; In the F2 treatment (Huaduoduo 1+Genniu 700× solution), the comprehensive evaluation value (D value) and ranking of flowering related indicators of Phalaenopsis aphrodite were the highest, and its comprehensive flowering quality was the highest which indicated that the Genniu amino acid water-soluble fertilizer has the strongest positive effect on promoting flowering plant growth of Phalaenopsis aphrodite. This study has guiding significance for selection of fertilizer and fertilization methods in Phalaenopsis aphrodite production.

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    Analysis of miRNA expression profiles at different developmental stages of Cnaphalocrocis medinalis
    ZHU Yu, LIU Yang
    2025, 66 (4):  979-985.  DOI: 10.16178/j.issn.0528-9017.20240744
    Abstract ( 10 )   HTML ( 1 )   PDF (2481KB) ( 11 )  

    This study analyzed the expression profiles of miRNAs in fifth-instar larvae and pupae of Cnaphalocrocis medinalis using high-throughput sequencing technology, and predicted the target genes of significantly differentially expressed miRNAs and performed functional enrichment analysis. In the miRNA library of fifth-instar larvae and pupae of C. medinalis, 44 miRNAs were present. There were 11 differentially expressed miRNAs between the fifth-instar larval and pupal libraries, with a total of 756 predicted target genes. Functional analysis of the target genes showed that these target genes were significantly enriched to 14 GO terms. This study not only provided miRNA expression profile and differential expression information during the development of fifth-instar larvae and pupae of C. medinalis, but also laid a foundation for the study of the function of miRNA in regulating insect development.

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    Evaluation of different adjuvants on the control effect of rice diseases and insect pests by spraying drone
    LUO Qin, YAO Xiaoming, SHOU Menghui, ZHOU Yujie
    2025, 66 (4):  986-991.  DOI: 10.16178/j.issn.0528-9017.20240067
    Abstract ( 7 )   HTML ( 0 )   PDF (1131KB) ( 13 )  

    In order to clarify the role of adjuvants in the control of rice diseases and insect pests by spraying drone five adjuvants, including Huoniu, Huainongte, Jiexiaoli, Jijian and Yafeng were selected to evaluate the control effect of "three pests and two diseases" such as rice planthopper and Chilo suppressalis. The results showed that adding 30 mL Huainongte, 30 mL Jiexiaoli or 15 mL Jijian per 667 m2 could improve the control effect of rice planthopper, Chilo suppressalis, Cnaphalocrocis medinalis, and Ustilaginoidea virens. The control effects of rice planthopper after 20 days of two times of spraying were 97.09%, 97.74% and 97.51%, respectively, which were 2.02, 2.67, and 2.44 percentage points higher than the single use of the insecticide. The control effects of Chilo suppressalis were 73.88%, 70.99% and 73.81%, respectively, which were 6.17, 3.28 and 6.10 percentage points higher than the single use of the insecticide. The control effects of Cnaphalocrocis medinalis were 88.99%, 88.43% and 89.06%, respectively, which were 1.09, 0.53 and 1.16 percentage points higher than the single use of the insecticide. The control effects of Ustilaginoidea virens were 86.21%, 85.93% and 88.26%, respectively, which were 0.36, 0.08 and 2.41 percentage points higher than the single use of the fungicide. However, there was no significant difference between the control effects of the above three adjuvants and the single use of the insecticide or fungicide. When adding 10 mL of Huoniu, 30 mL of Huainongte or 10 mL of Yafeng per 667 m2 for Thanatephorus cucumeris, the control effects were 76.53%, 74.11% and 73.75%, respectively, which were 2.94, 0.52 and 0.16 percentage points higher than those of the single use of the fungicide, but there was no significant difference between them. In conclusion, the adjuvants can improve the prevention and control effect of spraying drone on rice diseases and insect pests to a certain extent, but the effect is not significant on the basis of spraying spraying drone 2 L per 667 m2.

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    Evaluation of the field control efficacy of five ant bait insecticides against Solenopsis invicta
    CHEN Meiyun, QIAN Linghan, GUAN Yun, ZHANG Songyang, LI Qu, HUANG Fengyao, LIN Xiaojun, LI Shenlei
    2025, 66 (4):  992-1000.  DOI: 10.16178/j.issn.0528-9017.20240952
    Abstract ( 8 )   HTML ( 0 )   PDF (4123KB) ( 13 )  

    To explore the differences in the control efficacy of compound bait agents and single-agent bait agents on Solenopsis invicta and screen out the compound bait agents suitable for the control of Solenopsis invicta. In this experiment, the long-term dynamic monitoring research method was adopted to measure the field control efficacy of 2 kinds of compound bait agents and 3 kinds of single bait agents. The differences in the control efficacy of the 2 kinds of compound bait agents and their corresponding single-agent bait agents on Solenopsis invicta within 49 days were studied, as well as the comparison of the persistence of the efficacy among the 5 ant bait agents. The experimental results showed that the bait agent containing 0.1% indoxacarb had the best control effect on Solenopsis invicta, with a comprehensive control effect of 84.29%; followed by the two compound bait agents (the bait agent containing 0.8% hydramethylnon-indoxacarb and the bait agent containing 0.5% hydramethylnon-spinosad)effect, whose comprehensive control efficacy were 81.81% and 78.67%, respectively. The comprehensive control of the bait agent containing 0.015% spinosad was 77.01%, and that of the bait agent containing 1% hydramethylnon was 76.77%. The control effect of the bait agent containing 0.8% hydramethylnon-indoxacarb was second only to that of the bait agent containing 0.1% indoxacarb, but it had the best persistence of efficacy. Although the control effect of the bait agent containing 0.5% hydramethylnon-spinosad was not as good as that of the bait agent containing 0.8% hydramethylnon-indoxacarb, its control intensity and persistence of efficacy were stronger than those of the two corresponding single bait agents. It can be seen that the bait agent containing 0.8% hydramethylnon-indoxacarb and the bait agent containing 0.5% hydramethylnon-spinosad have good field control efficacy on Solenopsis invicta, and their control efficacy are not worse than those of single bait agents, which can be used as candidate drugs for scientific drug selection and drug replacement in the prevention and control of Solenopsis invicta.

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    Pathogenicity evaluation of four pathogenic nematodes on Spodoptera exigua at pupal stage
    LIU Min, LIN Ya, ZHANG Jinming
    2025, 66 (4):  1001-1004.  DOI: 10.16178/j.issn.0528-9017.20240961
    Abstract ( 9 )   HTML ( 0 )   PDF (1105KB) ( 13 )  

    The pathogenicity of four entomopathogenic nematodes (EPNs) against the prepupae and pupae of Spodoptera exigua was tested in laboratory to screen the effective strains of EPNs for the control of S. exigua, and the effect of soil moisture on the pathogenicity of EPNs was evaluated. Results showed that four EPNs exhibited different pathogenicity to S. exigua at the prepupa and pupa stages. At the same dose, the lethal effects of All, X-7, and JY-90 strains of Steinernema carpocapsae on the prepupa of S. exigua were better than that of the LN2 strain of Heterorhabditis indica. When the dose of EPNs was 100 IJs per prepupa, the mortality of the X-7, JY-90, and All strains of S. carpocapsae exceeded 80%, whereas the mortality of the LN2 strain of H. indica was only about 40%. The pathogenicity of the four EPNs to S. exigua pupae was lower than that to the prepupae. When the dose of EPNs was 1 600 IJs per pupa, the mortality of the X-7, JY-90, and All strains of S. carpocapsae were 67.5%, 40.0%, 18.7%, respectively, and the mortality of the LN2 strain of H. indica was only 38.6%. The effects of two soil moisture levels, 15% and 20%, on the mortality rate of the X-7 strain were evaluated, with the X-7 strain exhibiting a higher mortality rate for pupae in soil with 20% moisture content, indicating that entomopathogenic nematodes have a good application prospect in the control of S. exigua.

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    Study on detecting honey quality based on FTIR technology
    LAI Zeping, LU Wenjing, CHEN Di, ZHANG Cen, XIAO Chaogeng, PAN Leiming, GU Xiuying, XU Shuangyang, ZHU Haojie, YE Qin
    2025, 66 (4):  1005-1010.  DOI: 10.16178/j.issn.0528-9017.20240019
    Abstract ( 9 )   HTML ( 0 )   PDF (1481KB) ( 13 )  

    At present, the detection of honey quality is mainly based on chemical methods. However, chemical analysis has drawbacks such as long cycles and the need for a large number of organic reagents. Therefore, this study aims to establish a rapid method for determining the quality of honey. Firstly, 546 honey samples mixed with 0%-90% fructose syrup were used as materials, and a mid-infrared spectroscopy model for glucose and fructose content in honey was established based on Fourier transform mid-infrared spectroscopy (FTIR) combined with partial least squares (PLS) to quickly analyze honey quality. The results showed that under the standard normal variable (SNV) preprocessing method, the glucose and fructose index models in honey constructed at a wave number of 800-1 200 cm-1 had the best performance. The correlation coefficient (r), the root mean square error of calibration (RMSEC), the root mean square error of prediction (RMSEP), and the average relative error of the glucose index model were 0.991 6、0.91、0.94 and 0.89%, respectively; The r, RMSEC, RMSEP, and average relative errors of the fructose index model were 0.993 5、1.03、0.99 and 1.51%, respectively. The feasibility of the established method is demonstrated, which is significant for effectively replacing traditional chemical methods, significantly reducing the expensive analysis costs of conventional analysis, improving operator health, and reducing environmental pollution.

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    Optimization of fermentation technology of honey vinegar by Gluconacetobacter xylinus
    CHEN Tao, LI Ningning, WANG Jin, LU Zhufeng, YU Dan, LI Jiayou
    2025, 66 (4):  1011-1019.  DOI: 10.16178/j.issn.0528-9017.20240058
    Abstract ( 11 )   HTML ( 0 )   PDF (4357KB) ( 16 )  

    Honey vinegar have nutritional value, soft flavor and pure honey aroma, which is a new type of acetic acid drink. In this study, Gluconacetobacter xylinus was fermented to prepare cellulose immobilized bacterial film, and the optimal preparation process was determined by single factor experiment. The jujube nectar and Gluconacetobacter xylinus bacterial film were used to optimize the fermentation process of honey vinegar. The results showed that the best conditions for the preparation of immobilized bacterial film suited for Gluconacetobacter xylinus were: inoculum amount of 10%, incubation temperature of 30 ℃, ethanol volume fraction of 4%. The surface of the immobilized bacterial film is smooth and flat, arranged in layers, and has a high-density network space structure. The best process conditions for the immobilized fermentation of honey vinegar were bacterial film amount of 8%, fermentation temperature of 31 ℃, initial ethanol volume fraction of 4%, and initial pH value of 4.0. The honey vinegar beverage prepared under these conditions has a golden color, moderate acidity, and a strong vinegar aroma and honey aroma. The study may provide theoretical guidance for improving the production technology of honey vinegar.

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    Comparison of maximum residue limits for tea pesticide between China and abroad
    PAN Yingjie, WANG Yueyuan, TANG Shiqin, NING Jianmei, LIU Qiming
    2025, 66 (4):  1020-1024.  DOI: 10.16178/j.issn.0528-9017.20240005
    Abstract ( 9 )   HTML ( 0 )   PDF (1117KB) ( 13 )  

    China is the largest tea producer in the world and the export volume ranks among the top. This paper systematically studied the current status of maximum residue limit standards for tea pesticide in China, and the latest developments in the maximum residue limit standards for tea pesticide in the Europe, Japan, Korea and America, then compared the differences of maximum residue limit standards for tea pesticide between China and abroad. The situations of China's tea exports in 2023 and the possible reasons were also analyzed. The problems and countermeasures of the maximum residue limit standards in China were put forward. It is of great significance for ensuring the quality and safety of Chinese tea, actively coping with the international green trade barriers, and enhancing the export competitiveness of Chinese tea.

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    Analysis and comparison of amino acid content in muscle of Anji Zhuli chicken
    XU Ying, ZHANG Yaxiong, ZHAO Wenfeng, CAO Yun, XIAO Yingping, WANG Wen, LYU Wentao
    2025, 66 (4):  1025-1029.  DOI: 10.16178/j.issn.0528-9017.20231229
    Abstract ( 6 )   HTML ( 0 )   PDF (1109KB) ( 14 )  

    This study detected the contents of inosinic acid, fat, water and hydrolyzed amino acids/free amino acids in the chest and leg muscles of Anji Zhulin chicken and compared them with white feathered broiler chickens. The results showed that the inosinic acid content of the chest and leg muscles of Anji Zhulin chicken was 1 324.55 and 1 578.23 mg·kg-1, respectively, and the fat content was 1.21% and 2.38%, respectively. The total amount of hydrolyzed amino acids in the chest and leg muscles was 22.94% and 19.06%, respectively, of which the total amount of essential amino acids was 9.28% and 7.40%, and the total amount of branched-chain amino acids was 4.53% and 3.59%, respectively. The taurine content in the leg muscles was 1.771 mg·kg-1, and the anserine and carnosine content in the chest muscles were 12 518 and 3 711 mg·kg-1, respectively. The above amino acid indicators were higher in Anji Zhulin chickens than in the white feathered broiler chickens, indicating that the chest and leg muscles of Anji Zhulin chickens had richer contents of inosinic acid, intramuscular fat, and amino acids compared to white feathered broiler chickens, with a more delicious flavor and richer nutrition. This study provides data support for revealing the nutritional value evaluation of Anji Zhulin chicken, and also provides theoretical basis for high-quality flavor and nutritional level of Anji Zhulin chicken.

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    Suggestions on plant allocation in farmland nitrogen and phosphorus ecological interception ditch system:a case study of ecological ditch in Wenzhou Area
    LIU Haiya, WU Changzhou, LIN Changyong, LIU Xiaoli, YE Zhenyu
    2025, 66 (4):  1030-1035.  DOI: 10.16178/j.issn.0528-9017.20240671
    Abstract ( 10 )   HTML ( 1 )   PDF (1267KB) ( 16 )  

    Plant allocation is the main process component of ecological ditch system, and also an important factor to determine the effect of ecological ditch interception purification and ecological restoration. This paper introduced some practices of plant allocation in farmland nitrogen and phosphorus ecological interception ditch system in Wenzhou Area, analyzed and discussed the key points and combination models of functional plant allocation in farmland nitrogen and phosphorus ecological interception ditch system, and put forward suggestions for management and protection of functional plants, in order to provide reference for current agricultural non-point source pollution control.

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    Study on the composting process, microbial succession, and nitrogen transformation of landscaping waste
    SHEN Chenlei
    2025, 66 (4):  1036-1041.  DOI: 10.16178/j.issn.0528-9017.20240902
    Abstract ( 9 )   HTML ( 0 )   PDF (6892KB) ( 13 )  

    Composting is an effective way to recycle and dispose of landscaping waste resources. Landscaping waste comes from various sources with significant differences in their properties, and the conditions required for composting also vary significantly. This study divided landscaping waste into grass debris and tree branches and leaves, and composted them separately. Temperature, humidity, pH value, electrical conductivity(EC), and microbial community succession were monitored during the composting process of landscaping waste to explore the nitrogen transformation of different types of landscaping waste compost in Shanghai. The result showed that the temperature of compost containing grass debris and tree branches and leaves rapidly increased after the start of composting. The highest temperature of grass debris compost reached 69.7 oC on the 12th day, while the highest temperature of tree branches and leaves compost reached 66.0 oC on the 5th days. The pH value of compost containing grass debris and tree branches and leaves showed a decreasing trend in the early stage of composting, followed by an increasing trend. The turning point of the pH value change trend for both types of compost was between the 14th and 20th day. During the composting process, the EC showed a trend of rapid increase in the early stage and then remained stable. The maximum EC value of grass debris compost was 6.50 mS·cm-1, and the maximum EC value of tree branch and leaf compost was 2.31 mS·cm-1. Further analysis found that two types of landscaping waste compost contained a large number of microorganisms related to organic matter degradation, mainly including Bacillus subtilis, Actinobacteria, Gammaproteobacteria, Bacteroides, etc. FAPROTAX functional prediction analysis showed that the nitrogen cycling metabolic function in the compost gradually increased with the progress of composting, and the functional metabolic abundance of nitrogen transformation cycle in tree branches and leaves compost increased more significantly. This study revealed the nitrogen transformation of landscaping waste from the perspective of microbial metabolism, providing technical support for the composting and disposal of landscaping waste.

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    Screening of on high efficiency passivators of heavy metal mercury and its rational application
    CHEN Zhenhua, ZHANG Xuefang
    2025, 66 (4):  1042-1047.  DOI: 10.16178/j.issn.0528-9017.20230035
    Abstract ( 11 )   HTML ( 1 )   PDF (2904KB) ( 18 )  

    Due to the increasing industrial activities, mining and smelting, misuse of pesticides and chemical fertilisers, the trend of metal contamination in agricultural soils is becoming increasingly serious due to many factors, such as the random disposal of waste piles. The aim is to prevent and control heavy metal mercury contamination in agricultural soils and to ensure the ecological safety of agricultural soils. Based on a literature review and previous research, five commercially available passivators were selected, and indoor soil culture simulations and field trials were used to screen for highly effective heavy metal Hg passivators and their appropriate application techniques. The application of soil passivation remediation materials in the soil culture simulation experiment promoted the conversion of free Hg to the stable state in the soil. The best results were obtained with the application of agricultural lime and seafoam (ratio 1:1), which was significantly lower than other passivators or combination formulations (P<0.05), and the application of organic passivators significantly improved rice growth indicators. The application of two ratios of passivators, agricultural lime and seafoam (ratio 1:1) and biochar and seafoam (ratio 1:1), was also effective in reducing the enrichment of Hg in rice seeds. The difference in passivation effect between the passivator treatments in the field trial was not significant (P>0.05). There was also no significant effect of the passivator treatments on rice growth indicators. No Hg enrichment was detected in the rice grains with the different passivators, indicating a high level of grain safety. Soil cultivation experiments and field trials showed that all five passivators and combination formulations significantly reduced the effective soil Hg content, with the best results achieved by applying agricultural lime and seafoam (ratio 1:1) at a rate of 3 000 kg·hm-2.

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    Investigation on germplasm resources of wild vegetable in Southwestern Zhejiang
    CHEN Chao, LIU Tingfu, CHEN Xiaoyang, ZHOU Dayun, PAN Yiming, ZHU Biao, MIAO Yeminzi
    2025, 66 (4):  1048-1052.  DOI: 10.16178/j.issn.0528-9017.20240022
    Abstract ( 11 )   HTML ( 1 )   PDF (1099KB) ( 18 )  

    In order to promote the protection and development of wild vegetable resources in southwestern Zhejiang, the investigation and research of wild vegetable resources in above area were carried out. Through field exploration and key area investigation, a total of 105 species of 88 genera and 44 families of common wild vegetables (excluding edible fungi and bamboo) were discovered. The number of wild vegetables in December and January was the lowest, with 28 and 34 species respectively, which was related to the continuous low temperature season in winter. The number of resources in April and May was the highest, with 104 and 98 species respectively. There was no significant difference in the number of wild vegetables in different altitudes, and there were more than 96 species among the four altitudes. The wild vegetable resources in southwestern Zhejiang are abundant and have a good prospect of exploitation and utilization.

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