不同形态氮素营养对四环素胁迫下水稻苗期生长的影响

doi:10.16178/j.issn.0528-9017.20230631

摘要/Abstract

摘要：

为了探讨不同形态氮素营养对四环素类抗生素胁迫下水稻苗期生长的影响,采用营养液培养方法,在 $NH 4 +$ -N和 $NO 3 -$ -N不同氮素形态下,设置不同剂量四环素胁迫水平,研究了水稻干物质累积、根冠比、叶面积以及叶绿素含量和抗氧化酶活性的变化规律。结果表明,不同形态氮素营养能够调控四环素胁迫对水稻生长的影响。其中在低含量四环素胁迫时, $NH 4 +$ -N有利于促进水稻生长,增加地上部和地下部干重。而 $NO 3 -$ -N处理下水稻地上部和地下部干重未受四环素含量的明显影响。随着四环素浓度增加, $NO 3 -$ -N处理下水稻叶绿素a和b含量呈降低趋势,但地上部和地下部全氮含量呈增加趋势;而 $NH 4 +$ -N处理下叶绿素a和b含量和植株氮素累积量差异不显著,地上部和地下部全氮含量在低含量四环素胁迫时最高。随着四环素含量增加,不同形态氮素下水稻POD活性呈增加趋势,而SOD活性在 $NH 4 +$ -N处理下变化不明显,在 $NO 3 -$ -N处理下甚至降低。由此可见,可以通过不同形态氮素营养来调控水稻植株对四环素类抗生素的胁迫作用。

关键词: 水稻, 氮素形态, 四环素, 干物质累积, 叶绿素

Abstract:

To investigate the effects of different forms of nitrogen nutrition on the growth of rice seedlings under tetracycline antibiotic stress, a nutrient solution culture method was employed. Under different nitrogen forms ( $NH 4 +$ -N and $NO 3 -$ -N), various levels of tetracycline stress were applied to study the changes in dry matter accumulation, root-shoot ratio, leaf area, chlorophyll content, and antioxidant enzyme activities in rice. The results showed that different forms of nitrogen nutrition could regulate the impact of tetracycline stress on rice growth. Under low-dose tetracycline stress, $NH 4 +$ -N was beneficial for promoting rice growth and increasing dry weight in both aboveground and underground parts. In contrast, under $NO 3 -$ -N treatment, the dry weight in both aboveground and underground parts was not significantly affected by tetracycline content. As the tetracycline content increased, the content of chlorophyll a and b decreased under $NO 3 -$ -N treatment, while the total nitrogen content in both aboveground and underground parts increased. Under $NH 4 +$ -N treatment, the content of chlorophyll a and b and plant nitrogen accumulation did not show significant differences, with the highest total nitrogen content in both aboveground and underground parts observed under low-content tetracycline. As the tetracycline content increased, the POD activity in rice increased under different forms of nitrogen nutrition, while SOD activity showed no significant change under $NH 4 +$ -N treatment and even decreased under $NO 3 -$ -N treatment. These findings suggest that the stress effects of tetracycline on rice plants can be regulated through different forms of nitrogen nutrition.

Key words: rice, nitrogen forms, tetracycline, dry matter accumulation, chlorophyll

中图分类号:

S511

陈敏, 孙罗明昇, 沈佳铭, 叶友翔, 赵国华. 不同形态氮素营养对四环素胁迫下水稻苗期生长的影响[J]. 浙江农业科学, 2025, 66(6): 1322-1327.

CHEN Min, SUN-LUO Mingsheng, SHEN Jiaming, YE Youxiang, ZHAO Guohua. Effects of different forms of nitrogen nutrition on the growth of rice seedlings under tetracycline stress[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(6): 1322-1327.

图/表 7

图1 不同处理水稻地上部和地下部干重柱子上无相同小写字母表示处理间差异显著(P<0.05),图2~5同。

Fig.1 Dry weight of aboveground and underground parts of rice under different treatments

图2 不同处理水稻幼苗的根冠比

Fig.2 Root-shoot ratio of rice seedlings under different treatments

图3 不同处理的水稻叶面积

Fig.3 Leaf area of rice under different treatments

表1 不同处理水稻的叶绿素a和叶绿素b含量

Table 1 Contents of chlorophyll a and chlorophyll bin rice under different treatments 单位:mg·g-1

处理	叶绿素a含量	叶绿素b含量
T1	0.744 a	0.518 ab
T2	0.738 a	0.546 a
T3	0.762 a	0.513 ab
T4	0.649 ab	0.477 ab
T5	0.645 ab	0.442 b
T6	0.526 b	0.361 c

图4 水稻地上部和地下部总氮累积量

Fig.4 Total nitrogen accumulation in aboveground and underground parts of rice

图5 不同处理水稻地上部和地下部全氮含量

Fig.5 Total nitrogen content in aboveground and underground parts of rice under different treatments

表2 不同处理水稻幼苗SOD和POD活性

Table 2 SOD and POD activities of rice seedlings under different treatments

处理	POD活性/(U·g^-1)	SOD活性/(U·g^-1)
T1	23.90 c	403.74 b
T2	35.05 bc	389.96 b
T3	42.50 ab	394.34 b
T4	35.83 bc	458.68 a
T5	36.18 bc	390.63 b
T6	51.65 a	417.25 b

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