香榧籽油中金松酸提取制备工艺研究

doi:10.16178/j.issn.0528-9017.20230175

摘要/Abstract

摘要：

香榧籽油营养丰富,保健功能高。文章旨在探究香榧籽油中金松酸的最适宜的提取工艺。运用尿素包埋法,在单因素试验的基础上,采用响应面法对香榧籽油中金松酸的提取制备工艺进行优化。结果表明,尿素包埋提取香榧籽油中金松酸的最佳实验条件为:结合时间2.07 h、尿素质量33 g、结合温度68.93 ℃,在此条件下,香榧籽油中的金松酸最佳提取量是4.53 g。

关键词: 香榧籽油, 金松酸, 尿素包埋, 响应面法, 提取

Abstract:

Chinese torreya seed oil is rich in nutrients and has high health care functions. The purpose of this study was to explore the most suitable extraction process of sciadonic acid from Chinese torreya seed oil. The urea embedding method was used to optimize the extraction and preparation process of sciadonic acid from Chinese torreya seed oil by response surface method on the basis of single factor test. The results showed that the optimal experimental conditions for urea-embedded extraction of sciadonic acid from Chinese torreya seed oil were as follows: binding time 2.07 h, urea 33 g, binding temperature 68.93 ℃, and the optimal extraction amount of sciadonic acid from Chinese torreya seed oil was 4.53 g.

Key words: Chinese torreya seed oil, sciadonic acid, urea embedding, response surface method, extraction

中图分类号:

S565.4

姚诗炜, 卢红伶, 蒋陈凯, 胡文君, 于少芳, 相兴伟, 沈国新, 陈琳. 香榧籽油中金松酸提取制备工艺研究[J]. 浙江农业科学, 2024, 65(9): 2179-2183.

YAO Shiwei, LU Hongling, JIANG Chenkai, HU Wenjun, YU Shaofang, XIANG Xingwei, SHEN Guoxin, CHEN Lin. Preparation process of sciadonic acid extraction in Chinese torreya seed oil[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(9): 2179-2183.

图/表 10

表1 主要仪器与设备

Table 1 Main instruments and equipment

仪器	型号
电子天平	AL204-IC型
磁力搅拌器	522G型
抽滤真空泵	SHB-Ⅲ-A型
旋转蒸发仪	RE 2000型
气相色谱仪	Agilent 7890A型

表2 试验因素和水平

Table 2 Test factors and levels

水平	因素与水平
水平	A结合时间/h	B尿素质量/g	C结合温度/℃
-1	1.0	20	60
0	1.5	30	70
1	2.0	40	80

图1 不同结合时间对金松酸提取量的影响

Fig.1 Effect of different binding time on the extraction amount of sciadonic acid

图2 不同尿素质量对金松酸提取量的影响

Fig.2 Effect of different urea dose on the extraction amount of sciadonic acid

图3 不同结合温度对金松酸提取量的影响

Fig.3 Effect of different binding temperatures on the extraction amount of sciadonic acid

表3 Box-Behnken实验结果

Table 3 Results of the Box-Behnken experiment

实验号	A	B	C	提取量/g
1	-1	-1	0	2.0
2	1	-1	0	2.3
3	-1	1	0	3.4
4	1	1	0	3.7
5	-1	0	-1	2.9
6	1	0	-1	3.2
7	-1	0	1	2.5
8	1	0	1	2.9
9	0	1	-1	2.2
10	0	-1	-1	3.2
11	0	1	1	1.7
12	0	1	1	2.7
13	0	0	0	4.1
14	0	0	0	4.5
15	0	0	0	4.4
16	0	0	0	4.5
17	0	0	0	4.6

表4 回归方程方差分析

Table 4 Analysis of variance of the regression equation

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	14.05	9	1.56	45.44	<0.000 1	^**
A-结合时间	0.21	1	0.21	6.15	0.042 2	^*
B-尿素浓度	2.88	1	2.88	83.83	<0.000 1	^**
C-结合温度	0.36	1	0.36	10.51	0.014 2	^*
AB	0	1	0	0	1.000 0
AC	2.50×10^-3	1	2.50×10^-3	0.07	0.795 1
BC	0	1	0	0	1.000 0
A²	1.38	1	1.38	40.17	0.000 4	^*
B²	4.19	1	4.19	121.94	<0.000 1	^**
C²	3.98	1	3.98	115.90	<0.000 1	^**
残差	0.24	7	0.03
失拟	0.09	3	0.03	0.83	0.541 3
纯误差	0.15	4	0.04
总和	14.29	16

图4 结合时间与尿素质量交互作用影响金松酸提取提取量的响应面图

Fig.4 Response surface diagram of the interaction between binding time and urea dose on the extraction amount of sciadonic acid

图5 结合温度与结合时间交互作用影响金松酸提取量的响应面图

Fig.5 Response surface diagram of the interaction between binding temperature and binding time on the extraction amount of sciadonic acid

图6 尿素质量与结合温度交互作用影响金松酸提取提取量的响应面图

Fig.6 Response surface diagram of the interaction between urea dose and binding temperature on the extraction amount of sciadonic acid

参考文献 18

[1]	CUI H X, DUAN F F, JIA S S, et al. Antioxidant and tyrosinase inhibitory activities of seed oils from Torreya grandis fort. ex Lindl[J]. BioMed Research International, 2018, 2018: 5314320.
[2]	吴孝元, 陈琳, 蒋陈凯, 等. 加工香榧资源金珠榧1号的性状调查[J]. 浙江农业科学, 2021, 62(12): 2410-2411.
[3]	于美, 张川, 曾茂茂, 等. 香榧坚果中油脂和蛋白质的研究进展[J]. 食品科学, 2016, 37(17): 252-256.
[4]	HE Z Y, ZHU H D, LI W L, et al. Chemical components of cold pressed kernel oils from different Torreya grandis cultivars[J]. Food Chemistry, 2016, 209: 196-202.
[5]	CHEN S J, CHUANG L T, LIAO J S, et al. Phospholipid incorporation of non-methylene-interrupted fatty acids (NMIFA) in murine microglial BV-2 cells reduces pro-inflammatory mediator production[J]. Inflammation, 2015, 38(6): 2133-2145.
[6]	CHEN L, JIANG Q H, LU H L, et al. Antidiabetic effect of sciadonic acid on type 2 diabetic mice through activating the PI3K-AKT signaling pathway and altering intestinal flora[J]. Frontiers in Nutrition, 2022, 9: 1053348.
[7]	ENDO Y, TSUNOKAKE K, IKEDA I. Effects of non-methylene-interrupted polyunsaturated fatty acid, sciadonic (all-cis-5, 11, 14-eicosatrienoic acid) on lipid metabolism in rats[J]. Bioscience, Biotechnology, and Biochemistry, 2009, 73(3): 577-581.
[8]	罗凡, 郭少海, 杜孟浩, 等. 预处理条件对香榧仁油品质的影响研究[J]. 中国粮油学报, 2021, 36(4): 70-75.
[9]	叶玉兰. 藏牦牛酥油基制备母乳脂肪替代脂的研究[D]. 南昌: 南昌大学, 2016.
[10]	温思思, 陆元超, 丁珏, 等. 金松酸对HepG2细胞中甘油三酯蓄积的影响[J]. 中国粮油学报, 2022, 37(11): 191-198.
[11]	吕秋楠, 史高峰, 陈学福, 等. 尿素包合法纯化蚕蛹油中α-亚麻酸工艺研究[J]. 中国油脂, 2009, 34(8): 32-35.
[12]	郭江涛, 张永萍, 刘杰, 等. 大果木姜子不同部位挥发油成分及其抗氧化活性分析[J]. 食品工业科技, 2023, 44(17): 306-315.
[13]	董雪容, 孟雨薇, 周军, 等. 响应面法优化姜黄油脂质体制备工艺及性能的研究[J]. 中国粮油学报, 2022, 37(10): 199-205.
[14]	南阳, 张华, 刘国琴. 尿素包埋法富集椰子油中月桂酸甲酯[J]. 中国油脂, 2016, 41(3): 26-29.
[15]	陈琳, 卢红伶, 王少军, 等. 香榧油精炼温度的优化及其对油脂品质的影响[J]. 中国粮油学报, 2019, 34(2): 73-78.
[16]	QAMBRANI S, TALPUR F N, PANHWAR A A, et al. Development of guar gum-based coating with castor oil for improved postharvest quality of fresh mangoes using response surface methodology[J]. Applied Food Research, 2022, 2(2): 100220.
[17]	SAKDASRI W, SILA-NGAM P, CHUMMENGYEN S, et al. Optimization of yield and thymoquinone content of screw press-extracted black cumin seed oil using response surface methodology[J]. Industrial Crops and Products, 2023, 191: 115901.
[18]	段亚云, 李建颖, 程瑶, 等. 响应面法优化超声波-微波协同提取黑果枸杞叶总黄酮工艺[J]. 食品研究与开发, 2018, 39(21): 100-105.