黄秋葵多糖提取、分离纯化及结构分析方法研究进展

doi:10.16178/j.issn.0528-9017.20220336

摘要/Abstract

摘要：

黄秋葵为锦葵科一年生草本植物,其嫩果富含类黄酮、多酚、氨基酸、多糖等生物活性物质,为人们熟知的保健蔬菜。食用黄秋葵具有一定的抗氧化、抗肿瘤、抗癌、抗糖尿病以及免疫调节作用,黄秋葵多糖被认为是与上述生物活性相关的物质。黄秋葵多糖的提取、分离纯化以及结构分析是开展生物活性研究的前提,本文对目前黄秋葵的提取、分离纯化和结构分析进行阐述,以期为黄秋葵多糖研究提供参考。

关键词: 黄秋葵多糖, 提取, 分离纯化, 结构分析

中图分类号:

S649
Q539

王小秋, 仇亮, 翟彩娇, 宋益民, 程玉静, 刘水东. 黄秋葵多糖提取、分离纯化及结构分析方法研究进展[J]. 浙江农业科学, 2023, 64(3): 694-704.

图/表 1

参考文献 112

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[109]	张鲜艳, 崔红利, 焦稚雅, 等. 不同秋葵品种营养品质分析[J]. 山西农业大学学报(自然科学版), 2020, 40(2): 1-7.
[110]	王曼宇, 刘乃新, 张福顺. 植物源性多糖提取及生物活性研究进展[J]. 中国农学通报, 2021, 37(29): 34-41. DOI
[111]	方积年, 丁侃. 天然药物: 多糖的主要生物活性及分离纯化方法[J]. 中国天然药物, 2007, 5(5): 338-347.
[112]	薛志忠, 刘思雨, 杨雅华. 黄秋葵的应用价值与开发利用研究进展[J]. 保鲜与加工, 2013, 13(2): 58-60.

试验材料	提取方法		纯化方法						提取率/%		平均分子量/u
秋葵叶片	热水浸提法		TCA、DEAE-cellulose、DEAE-Sephadex						7.1		1.9×10⁵
秋葵嫩果	超声波辅助法		DEAE-cellulose						13.99±0.52		2.19×10⁵
秋葵种子	热水浸提法		DEAE-Sephadex						NA		NA
秋葵果荚	NA		NA						NA		2.174×10⁶
秋葵果荚	热水浸提法		HP-5 column、DEAE-Sepharose、超滤膜(100 kDa)						1.10		>2.99×10⁶
秋葵叶片	超声波辅助法		NA						3.11		26.9×10³
木质化秋葵果荚	热水浸提法		Sevage、DEAE cellulose						0.50		6.59×10⁵
秋葵果荚	超声波辅助法		DEAE cellulose						NA		2.84×10⁵
秋葵花	水浸提法		DEAE cellulose、DEAE-Sephacryl						NA		1.7×10⁵
秋葵花	热水浸提法		DEAE cellulose、DEAE-Sephacryl						15.36		2.741×10⁵
秋葵果荚	热水浸提法		DEAE cellulose						NA		5 198
秋葵果荚	热水浸提法		DEAE cellulose						NA		2 121 804
秋葵果荚	热水浸提法		DEAE celluloseDEAE-Sephacryl						NA		9.12×10⁴
秋葵果荚	碱提法		DEAE cellulose、Sephacryl						NA		9.79×10⁴
秋葵果荚	热水浸提法		DEAE-cellulose、DEAE-Sepharose						7.90		5.8×10⁵
秋葵果荚	热水浸提法		DEAE-Sepharose						0.59		NA
秋葵果荚	微波辅助法(20 kHz)		Sevage						13.14±0.05		7.28~732.83×10⁵
秋葵果荚	微波辅助法(40/60 kHz)		Sevage						14.15±0.04		0.85~714.93×10⁵
秋葵果荚	热水浸提法		HP-5 column						14.6±1.2		1.202×10⁶
秋葵果荚	超声波辅助法		DEAE-Sepharose						10.35±0.11		1.92×10⁵
秋葵果荚	超声波辅助法		DEAE-cellulose						NA		3.86×10⁵
秋葵果荚	热水浸提法		NA						NA		NA
秋葵果荚	螯合剂法		NA						NA		NA
秋葵果荚	热水浸提法		DEAE-cellulose、DEAE-Sephadex						8.60		8.8×10³
秋葵果荚	热水浸提法		DEAE-Sepharose、TCA						1.3		1 380
秋葵果肉	热水浸提法		NA						29.40		6 436×10³~7 432×10³
秋葵种子	水提醇沉法		DEAE-Sephadex、透析						NA		191 838.9
秋葵茎秆	超声波辅助法		乙醇和硫酸铵的双水相萃取体系						23.59		NA
秋葵籽	热水浸提法		DEAE cellulose、DEAE-Sephacryl						5.82		7.09×10⁵
秋葵果皮	热水浸提法		Sevag,透析48 h脱盐						NA		NA
秋葵果皮	螯合剂提取法		Sevag,透析48 h脱盐						NA		NA
秋葵果皮	碱提取法		Sevag,透析48 h脱盐						NA		NA
秋葵果荚	超声波辅助法		NA						6.88		NA
秋葵籽	热水浸提法		Sevage、DEAE-cellulose、DEAE-Sephacryl						5.82		7.09×10⁵
试验材料	单糖组成分析方法		单糖组成及摩尔质量百分比				可能存在结构						啊啊啊啊
秋葵叶片	GC-MS		D-Ara∶D-Xyl∶D-Glc∶D-Man∶D-Gal=2.9∶3.1∶0.3∶0.4∶3.4				NA						[7]
秋葵嫩果	HPLC、FT-IR、NMR		Rha∶GalA∶Gal=1.01∶1.00∶2.31				主链:-4)-α-d-GalAp-(1,2,4)-α-l-Rhap-(1-galactan侧链部分O-4被Rhap取代						[75]
秋葵种子	HPLC		Rha, Ara, D-Xyl, D-Fru和D-Glc为主要成分				NA						[76]
秋葵果荚	GC-MS HPLC FT-IR		Rha∶Ara∶Glc∶Gal∶GalA=4.75∶2.01∶1.00∶4.91∶7.24				1,4-linked galacturonan units (homogalacturonan backbone) and (1→2) and (1→2,4) linked Rha (rhamnogalacturonan I region). And the other side chains contained→1)-linked Ara, (1→5)-linked Ara, (1→4)-linked Glc, (1→6)-linked Gal, (1→4)-linked Rha, (1→2,4)-linked Rha,→1)-linked Ara and→1)-linked Gal						[11]
秋葵果荚	GC、NMR		Ara∶Gal=1.0∶18.3,Rha和GalA相近				主链:→4)-D-GalpAMe-(1→2)-L-Rhap-(1→侧链:Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→Araf-α-L-(1→6)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→						[77]
秋葵叶片		FT-IR、NMR			Gal∶GalA∶Rha∶Ara≈10.8∶5.8∶1.8∶1			多分支、高度乙酰化和未甲基化多糖						[78]
木质化秋葵果荚		FT-IR、NMR、GC			Gal∶Rha∶Glc∶Ara∶GalA=1.98∶1.00∶0.15∶0.32∶0.29			→2)-α-D-Rhap-(1→,→4)-β-D-Galp-(1→,→4)-α-D-GalpA-(1→,→6)-β-D-Galp-(1→, β-D-Glcp-(1→and α-L-Araf-(1→						[79]
秋葵果荚		FT-IR、NMR			Rha∶Gal∶GalA∶Man∶Ara=1∶2.3∶1.03∶0.42∶0.23			主链:-4)-α-D-GalAp-(1,2,4)-α-L-Rhap-(1-侧链:β-D-Galp-(1,4)-β-D-Galp-(1-or α-L-Araf-(1, 4)-β-D-Galp-(1-重复单元						[80]
秋葵花		GC、FT-IR			Gal∶Rha=2.23∶1和少部分Ara\Glc和Man			NA						[81]
秋葵花		FT-IR、NMR、PMP-HPLC-UV			Rha∶GalA∶Gal=1∶∶1.02∶0.86			2)-α-D-Rhap-(1→4)-α-D-GalpA-(1→2,4)-α-D-Rhap-(1→4)-α-D-GalpA-(1						[55]
秋葵果荚		HPLC、FT-IR、NMR			Man∶Rha∶GluA,∶GalA∶Glc∶Ara=1.0∶0.9∶3.5∶3.5∶11.7∶1.0			T-linked-Araf, 1,4-linked-Glcp, 1,6-linkedGlcp, 1,4-linked-GalAp, 1,2-linked-Rhap, 1,6-linked-Manp, 1,4-linked-GlcAp and 1,2,4-linked-Rhap.						[82]
秋葵果荚		HPLC、FT-IR、NMR			Man∶Rha∶GalAc∶Glc∶Gal=1.0∶ 8.0∶3.2∶1.2∶15.0			1,3-,1,6-, 1,3,6-linkedGalp. The branched structures in AEP-2include 1,2-linked Rhap, 1,4-linked GalA						[82]
秋葵果荚		HPLC、NMR			Gal∶Rha∶Fru∶Ara∶Glc∶GalA∶Mann=32.53∶19.76∶17.21∶13.38∶8.94∶5.24∶2.94			NA						[83]
秋葵果荚		HPLC、NMR			Man∶Rha∶GalA∶Gal=0.84∶3.12∶2.15∶5.89.			NA						[83]
秋葵果荚		PMP-HPLC、NMR			Rha∶GalA∶Gal∶GlcA∶Glc∶Ara=21.4∶34.9∶29.6∶4.5∶5.9∶3.7			鼠李半乳糖醛酸聚糖-I为主链,鼠李半乳糖醛酸聚糖-II中阿拉伯半聚乳糖侧链部分O-4被Rhap取代						[84]
秋葵果荚		HPLC			Man∶Rha∶GluA∶GalA∶Gal∶Ara=3.4∶3.76∶24.19∶6.27∶8.73∶3.13			NA						[85]
秋葵果荚		IC、FT-IR			Rha∶Ara∶Gal∶Glc∶GluA=4.7±0.06∶1.6±0.05∶34.1±0.15∶50.3±0.22∶9.4±0.06			NA						[86]
秋葵果荚		IC、FT-IR			Rha∶Ara∶Gal∶Glc∶GluA=11.6±0.09∶5.7±0.05∶55.5±0.14∶14.7±0.12∶12.4±0.08			NA						[86]
秋葵果荚		GC-MS、FT-IR、NMR			D-Gal∶L-Rha∶L-Ara∶D-Glc=18.4∶11.0∶2.3∶0.9			NA						[87]
秋葵果荚		GC、FTIR、HPLC			Glc∶Man∶Gal∶Ara∶Xyl∶Fru∶Rha=28.8∶12.5∶13.1∶15.9∶9.2∶13.7∶6.8			NA						[88]
秋葵果荚		RP-HPLC、FT-IR			Gal∶GalA∶Glc∶Rha∶GlcA∶Man∶Ara∶Fuc∶Xyl=50.5∶25.9∶8.2∶7.4∶2.9∶2.1∶1.9∶0.6∶0.5			主链:鼠李半乳糖醛酸聚糖-I,长半乳糖侧链						[89]
秋葵果荚		ESI-IT-MS、NMR			Rha∶Gal∶Glu∶GalA∶GlcA=26∶34∶1∶35∶3			主要为鼠李半乳糖醛酸聚糖-I,侧链上为短半乳糖残基(85%)和少量同型半乳糖醛酸聚糖1,2,4-linked a-Rhap\1,4-linked a-GalpA (RG I)\t-a Galp\1,4-linked Galp\t-b-Galp						[90]
秋葵果荚		ESI-IT-MS、NMR			Rha∶Ara∶Gal∶Glu∶GalA∶GlcA=14∶3∶17∶1∶63∶2			主要为同型半乳糖醛酸聚糖,少量鼠李半乳糖醛酸聚糖-I(24%)和1,2-linked-和1,2,4-linked rhamnosyl重复单元, O-3替换为O-acetylated						[90]
秋葵果荚		GC-MS、FT-IR、NMR、 HPLC			β-D-glc∶α-D-Man∶α-D-Gala∶α-L-Fuc=1.00∶0.91∶2.14∶1.09			→6)α-D-Galp-(1→6)α-D-Manp-(1→6)α-D-Galp-(1→重复和分支β-D-Glcp(1→3)α-Fucp-(1→;O-3被Man替代						[91]
秋葵果荚				HPLC、GC-MS		Rha∶Ara∶Xyl∶Man∶Gal∶Glc=19.9∶2.1∶1.3∶0.5∶18.2∶3.7				NA		[58]
秋葵果肉				HPLC、FT-IR		Gal∶Rha∶GalA∶Xyl∶Ara=7.3∶2.9∶2.5∶1∶1.1				NA		[92]
秋葵种子				HPLC		Rha、Ara、D-Xyl、D-Fru、D-Glc				NA		[64]
秋葵茎秆				HPLC		GlcA、Xyl、Fuc、GalA和Ara				NA		[68]
秋葵籽				GC		Man∶Gal∶Xyl∶Ara∶Rha=38.06∶30.38∶17.77∶11.02∶2.78				NA		[66]
秋葵果皮				HPLC		Man∶Rha∶GlcA∶Gal=0.1∶7.0∶0.4∶5.8∶0.9∶14.6∶1.5				NA		[61]
秋葵果皮				HPLC		Rha∶GalA∶Gal∶Ara=4.7∶61.8∶9.6∶5.3				NA		[61]
秋葵果皮				HPLC		Man∶Rha∶GalA∶Glc∶Gal∶Ara=9.5∶5.4∶1.3∶10.8∶2.4∶7.6				NA		[61]
秋葵果荚				HPLC		Rha∶GlcA∶Man∶Glc=34.44∶24.12∶10.84∶4.68				NA		[93]
秋葵籽				高碘酸氧化、Smith降解、FT-IR		Man∶Gal∶Xyl∶Ara∶Rha=36.98∶31.29∶15.61∶8.87∶3.37				含有甘露糖残基,1→、1→6、1→2、1→2,6糖苷键		[65]

试验材料	提取方法		纯化方法						提取率/%		平均分子量/u
秋葵叶片	热水浸提法		TCA、DEAE-cellulose、DEAE-Sephadex						7.1		1.9×10⁵
秋葵嫩果	超声波辅助法		DEAE-cellulose						13.99±0.52		2.19×10⁵
秋葵种子	热水浸提法		DEAE-Sephadex						NA		NA
秋葵果荚	NA		NA						NA		2.174×10⁶
秋葵果荚	热水浸提法		HP-5 column、DEAE-Sepharose、超滤膜(100 kDa)						1.10		>2.99×10⁶
秋葵叶片	超声波辅助法		NA						3.11		26.9×10³
木质化秋葵果荚	热水浸提法		Sevage、DEAE cellulose						0.50		6.59×10⁵
秋葵果荚	超声波辅助法		DEAE cellulose						NA		2.84×10⁵
秋葵花	水浸提法		DEAE cellulose、DEAE-Sephacryl						NA		1.7×10⁵
秋葵花	热水浸提法		DEAE cellulose、DEAE-Sephacryl						15.36		2.741×10⁵
秋葵果荚	热水浸提法		DEAE cellulose						NA		5 198
秋葵果荚	热水浸提法		DEAE cellulose						NA		2 121 804
秋葵果荚	热水浸提法		DEAE celluloseDEAE-Sephacryl						NA		9.12×10⁴
秋葵果荚	碱提法		DEAE cellulose、Sephacryl						NA		9.79×10⁴
秋葵果荚	热水浸提法		DEAE-cellulose、DEAE-Sepharose						7.90		5.8×10⁵
秋葵果荚	热水浸提法		DEAE-Sepharose						0.59		NA
秋葵果荚	微波辅助法(20 kHz)		Sevage						13.14±0.05		7.28~732.83×10⁵
秋葵果荚	微波辅助法(40/60 kHz)		Sevage						14.15±0.04		0.85~714.93×10⁵
秋葵果荚	热水浸提法		HP-5 column						14.6±1.2		1.202×10⁶
秋葵果荚	超声波辅助法		DEAE-Sepharose						10.35±0.11		1.92×10⁵
秋葵果荚	超声波辅助法		DEAE-cellulose						NA		3.86×10⁵
秋葵果荚	热水浸提法		NA						NA		NA
秋葵果荚	螯合剂法		NA						NA		NA
秋葵果荚	热水浸提法		DEAE-cellulose、DEAE-Sephadex						8.60		8.8×10³
秋葵果荚	热水浸提法		DEAE-Sepharose、TCA						1.3		1 380
秋葵果肉	热水浸提法		NA						29.40		6 436×10³~7 432×10³
秋葵种子	水提醇沉法		DEAE-Sephadex、透析						NA		191 838.9
秋葵茎秆	超声波辅助法		乙醇和硫酸铵的双水相萃取体系						23.59		NA
秋葵籽	热水浸提法		DEAE cellulose、DEAE-Sephacryl						5.82		7.09×10⁵
秋葵果皮	热水浸提法		Sevag,透析48 h脱盐						NA		NA
秋葵果皮	螯合剂提取法		Sevag,透析48 h脱盐						NA		NA
秋葵果皮	碱提取法		Sevag,透析48 h脱盐						NA		NA
秋葵果荚	超声波辅助法		NA						6.88		NA
秋葵籽	热水浸提法		Sevage、DEAE-cellulose、DEAE-Sephacryl						5.82		7.09×10⁵
试验材料	单糖组成分析方法		单糖组成及摩尔质量百分比				可能存在结构						啊啊啊啊
秋葵叶片	GC-MS		D-Ara∶D-Xyl∶D-Glc∶D-Man∶D-Gal=2.9∶3.1∶0.3∶0.4∶3.4				NA						[7]
秋葵嫩果	HPLC、FT-IR、NMR		Rha∶GalA∶Gal=1.01∶1.00∶2.31				主链:-4)-α-d-GalAp-(1,2,4)-α-l-Rhap-(1-galactan侧链部分O-4被Rhap取代						[75]
秋葵种子	HPLC		Rha, Ara, D-Xyl, D-Fru和D-Glc为主要成分				NA						[76]
秋葵果荚	GC-MS HPLC FT-IR		Rha∶Ara∶Glc∶Gal∶GalA=4.75∶2.01∶1.00∶4.91∶7.24				1,4-linked galacturonan units (homogalacturonan backbone) and (1→2) and (1→2,4) linked Rha (rhamnogalacturonan I region). And the other side chains contained→1)-linked Ara, (1→5)-linked Ara, (1→4)-linked Glc, (1→6)-linked Gal, (1→4)-linked Rha, (1→2,4)-linked Rha,→1)-linked Ara and→1)-linked Gal						[11]
秋葵果荚	GC、NMR		Ara∶Gal=1.0∶18.3,Rha和GalA相近				主链:→4)-D-GalpAMe-(1→2)-L-Rhap-(1→侧链:Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→Araf-α-L-(1→6)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→						[77]
秋葵叶片		FT-IR、NMR			Gal∶GalA∶Rha∶Ara≈10.8∶5.8∶1.8∶1			多分支、高度乙酰化和未甲基化多糖						[78]
木质化秋葵果荚		FT-IR、NMR、GC			Gal∶Rha∶Glc∶Ara∶GalA=1.98∶1.00∶0.15∶0.32∶0.29			→2)-α-D-Rhap-(1→,→4)-β-D-Galp-(1→,→4)-α-D-GalpA-(1→,→6)-β-D-Galp-(1→, β-D-Glcp-(1→and α-L-Araf-(1→						[79]
秋葵果荚		FT-IR、NMR			Rha∶Gal∶GalA∶Man∶Ara=1∶2.3∶1.03∶0.42∶0.23			主链:-4)-α-D-GalAp-(1,2,4)-α-L-Rhap-(1-侧链:β-D-Galp-(1,4)-β-D-Galp-(1-or α-L-Araf-(1, 4)-β-D-Galp-(1-重复单元						[80]
秋葵花		GC、FT-IR			Gal∶Rha=2.23∶1和少部分Ara\Glc和Man			NA						[81]
秋葵花		FT-IR、NMR、PMP-HPLC-UV			Rha∶GalA∶Gal=1∶∶1.02∶0.86			2)-α-D-Rhap-(1→4)-α-D-GalpA-(1→2,4)-α-D-Rhap-(1→4)-α-D-GalpA-(1						[55]
秋葵果荚		HPLC、FT-IR、NMR			Man∶Rha∶GluA,∶GalA∶Glc∶Ara=1.0∶0.9∶3.5∶3.5∶11.7∶1.0			T-linked-Araf, 1,4-linked-Glcp, 1,6-linkedGlcp, 1,4-linked-GalAp, 1,2-linked-Rhap, 1,6-linked-Manp, 1,4-linked-GlcAp and 1,2,4-linked-Rhap.						[82]
秋葵果荚		HPLC、FT-IR、NMR			Man∶Rha∶GalAc∶Glc∶Gal=1.0∶ 8.0∶3.2∶1.2∶15.0			1,3-,1,6-, 1,3,6-linkedGalp. The branched structures in AEP-2include 1,2-linked Rhap, 1,4-linked GalA						[82]
秋葵果荚		HPLC、NMR			Gal∶Rha∶Fru∶Ara∶Glc∶GalA∶Mann=32.53∶19.76∶17.21∶13.38∶8.94∶5.24∶2.94			NA						[83]
秋葵果荚		HPLC、NMR			Man∶Rha∶GalA∶Gal=0.84∶3.12∶2.15∶5.89.			NA						[83]
秋葵果荚		PMP-HPLC、NMR			Rha∶GalA∶Gal∶GlcA∶Glc∶Ara=21.4∶34.9∶29.6∶4.5∶5.9∶3.7			鼠李半乳糖醛酸聚糖-I为主链,鼠李半乳糖醛酸聚糖-II中阿拉伯半聚乳糖侧链部分O-4被Rhap取代						[84]
秋葵果荚		HPLC			Man∶Rha∶GluA∶GalA∶Gal∶Ara=3.4∶3.76∶24.19∶6.27∶8.73∶3.13			NA						[85]
秋葵果荚		IC、FT-IR			Rha∶Ara∶Gal∶Glc∶GluA=4.7±0.06∶1.6±0.05∶34.1±0.15∶50.3±0.22∶9.4±0.06			NA						[86]
秋葵果荚		IC、FT-IR			Rha∶Ara∶Gal∶Glc∶GluA=11.6±0.09∶5.7±0.05∶55.5±0.14∶14.7±0.12∶12.4±0.08			NA						[86]
秋葵果荚		GC-MS、FT-IR、NMR			D-Gal∶L-Rha∶L-Ara∶D-Glc=18.4∶11.0∶2.3∶0.9			NA						[87]
秋葵果荚		GC、FTIR、HPLC			Glc∶Man∶Gal∶Ara∶Xyl∶Fru∶Rha=28.8∶12.5∶13.1∶15.9∶9.2∶13.7∶6.8			NA						[88]
秋葵果荚		RP-HPLC、FT-IR			Gal∶GalA∶Glc∶Rha∶GlcA∶Man∶Ara∶Fuc∶Xyl=50.5∶25.9∶8.2∶7.4∶2.9∶2.1∶1.9∶0.6∶0.5			主链:鼠李半乳糖醛酸聚糖-I,长半乳糖侧链						[89]
秋葵果荚		ESI-IT-MS、NMR			Rha∶Gal∶Glu∶GalA∶GlcA=26∶34∶1∶35∶3			主要为鼠李半乳糖醛酸聚糖-I,侧链上为短半乳糖残基(85%)和少量同型半乳糖醛酸聚糖1,2,4-linked a-Rhap\1,4-linked a-GalpA (RG I)\t-a Galp\1,4-linked Galp\t-b-Galp						[90]
秋葵果荚		ESI-IT-MS、NMR			Rha∶Ara∶Gal∶Glu∶GalA∶GlcA=14∶3∶17∶1∶63∶2			主要为同型半乳糖醛酸聚糖,少量鼠李半乳糖醛酸聚糖-I(24%)和1,2-linked-和1,2,4-linked rhamnosyl重复单元, O-3替换为O-acetylated						[90]
秋葵果荚		GC-MS、FT-IR、NMR、 HPLC			β-D-glc∶α-D-Man∶α-D-Gala∶α-L-Fuc=1.00∶0.91∶2.14∶1.09			→6)α-D-Galp-(1→6)α-D-Manp-(1→6)α-D-Galp-(1→重复和分支β-D-Glcp(1→3)α-Fucp-(1→;O-3被Man替代						[91]
秋葵果荚				HPLC、GC-MS		Rha∶Ara∶Xyl∶Man∶Gal∶Glc=19.9∶2.1∶1.3∶0.5∶18.2∶3.7				NA		[58]
秋葵果肉				HPLC、FT-IR		Gal∶Rha∶GalA∶Xyl∶Ara=7.3∶2.9∶2.5∶1∶1.1				NA		[92]
秋葵种子				HPLC		Rha、Ara、D-Xyl、D-Fru、D-Glc				NA		[64]
秋葵茎秆				HPLC		GlcA、Xyl、Fuc、GalA和Ara				NA		[68]
秋葵籽				GC		Man∶Gal∶Xyl∶Ara∶Rha=38.06∶30.38∶17.77∶11.02∶2.78				NA		[66]
秋葵果皮				HPLC		Man∶Rha∶GlcA∶Gal=0.1∶7.0∶0.4∶5.8∶0.9∶14.6∶1.5				NA		[61]
秋葵果皮				HPLC		Rha∶GalA∶Gal∶Ara=4.7∶61.8∶9.6∶5.3				NA		[61]
秋葵果皮				HPLC		Man∶Rha∶GalA∶Glc∶Gal∶Ara=9.5∶5.4∶1.3∶10.8∶2.4∶7.6				NA		[61]
秋葵果荚				HPLC		Rha∶GlcA∶Man∶Glc=34.44∶24.12∶10.84∶4.68				NA		[93]
秋葵籽				高碘酸氧化、Smith降解、FT-IR		Man∶Gal∶Xyl∶Ara∶Rha=36.98∶31.29∶15.61∶8.87∶3.37				含有甘露糖残基,1→、1→6、1→2、1→2,6糖苷键		[65]

黄秋葵多糖提取、分离纯化及结构分析方法研究进展

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