衣藻对盐胁迫下小麦幼苗生长和生理指标的影响研究

doi:10.16178/j.issn.0528-9017.20230162

摘要/Abstract

摘要：

盐胁迫是植物面临的最主要非生物胁迫之一。对农业生产而言,研究生物肥料对盐胁迫下植物生理指标的影响具有重要意义。目前,藻类生物肥料对盐碱土壤的影响已被广泛研究,但是其对盐胁迫下植物生理指标的影响鲜有报道。以德巴衣藻(Chlamydomonas debaryana)藻粉作为生物肥料,通过实验模拟在高盐胁迫环境下,向小麦幼苗沙土中添加不同浓度的衣藻藻粉(0.25、0.5、1.0、2.0 g·L^-1),并测定了小麦生物量、可溶性糖含量、可溶性蛋白含量、抗氧化酶活性、丙二醛含量和离子平衡等相关指标。结果表明,盐胁迫使小麦根长、茎长、干重和鲜重等各项指标均受到显著抑制,可溶性糖含量和可溶性蛋白含量降低,抗氧化酶活性降低,丙二醛含量升高。添加不同浓度衣藻藻粉后,小麦根长、茎长、干重和鲜重等各项指标均有明显增长,可溶性糖含量和可溶性蛋白含量增加,抗氧化酶活性升高,丙二醛含量降低。相关分析表明,衣藻藻粉能够促进小麦生长,显著改变小麦生理指标,提升其耐盐性。综合比较,1 g·L^-1藻粉处理组效果最为显著。

关键词: 盐胁迫, 小麦, 衣藻藻粉, 生理指标

Abstract:

Salt stress is one of the most main abiotic stresses faced by plants. In terms of agricultural production, it is of great significance to study the effects of bio-fertilizers on plant physiological indices under salt stress. Currently, the effects of algae bio-fertilizer on salinized soil have been widely studied. However, its effects on plant physiological indices under salt stress remains to be unclear. Chlamydomonas debaryana powder was used as bio-fertilizer in this study. algae powder with different concentrations (0.25, 0.5, 1.0, 2.0 g·L^-1) were added to wheat seedling sandy soil under high salt stress, The biomass, soluble sugar content, soluble protein content, antioxidant enzyme activity, malondialdehyde content and ion balance of wheat were measured. The results showed that salt stress significantly inhibited the root length, stem length, dry weight and fresh weight of wheat, decreased the contents of soluble sugar and soluble protein, decreased the activity of antioxidant enzymes, and increased the content of malondialdehyde. The root length, stem length, dry weight and fresh weight of wheat increased significantly, the contents of soluble sugar and soluble protein increased, the activity of antioxidant enzymes increased, and the content of malondialdehyde decreased after algae powder was added at different concentrations. Correlation analysis showed that the algae powder could promote wheat growth, significantly change wheat physiological indices, and improve its salt tolerance. By comprehensive comparison, the effect of 1 g·L^-1 algae powder treatment group was the most significant.

Key words: salt stress, wheat, Chlamyceous debaryana powder, physiological index

中图分类号:

S512.1

王彦钦, 李武阳, 孟宪刚, 罗光宏. 衣藻对盐胁迫下小麦幼苗生长和生理指标的影响研究[J]. 浙江农业科学, 2024, 65(3): 497-504.

WANG Yanqin, LI Wuyang, MENG Xiangang, LUO Guanghong. Effects of Chlamydomonas debaryana powder on growth and physiological indices of wheat seedlings under salt stress[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(3): 497-504.

图/表 12

图1 藻粉对盐胁迫下小麦根长和茎长的影响

Fig.1 Effects of algae powder on root and stem length of wheat under salt stress

图2 藻粉对盐胁迫下小麦干重的影响

Fig.2 Effect of algae powder on dry weight of wheat under salt stress

图3 藻粉对盐胁迫下小麦鲜重的影响

Fig.3 Effect of algae powder on fresh weight of wheat under salt stress

图4 小麦根系扫描照片

Fig.4 Scanning photos of wheat roots

表1 小麦根系扫描数据

Table 1 Scanning data of wheat roots

处理组	总根表面积/cm²	平均根系直径/cm	分枝数	分叉数
CK	15.188±0.283 a	0.236±0.012 a	1 223.0±94.0 a	315.7±15.8 a
T0	6.242±0.995 c	0.237±0.002 a	344.3±34.0 d	87.0±11.6 c
T1	6.522±0.362 c	0.228±0.017 ab	607.3±99.4 c	106.7±14.7 c
T2	9.469±1.929 b	0.232±0.015 ab	601.0±58.3 c	123.7±9.4 c
T3	9.507±0.088 b	0.205±0.010 b	565.0±41.7 c	178±23.3 b
T4	10.372±1.116 b	0.227±0.006 ab	838.0±40.2 b	120.7±34.5 c

图5 藻粉对盐胁迫下小麦可溶性蛋白含量的影响同组柱上无相同小写字母表示不同处理间差异显著(P<0.05)。图6同。

Fig.5 Effect of algae powder on soluble protein content of wheat under salt stress

图6 藻粉对盐胁迫下小麦可溶性糖含量的影响

Fig.6 Effect of algae powder on soluble sugar content of wheat under salt stress

图7 藻粉对盐胁迫下小麦超氧化物歧化酶活性的影响同颜色柱上无相同小写字母表示同一处理不同时间差异显著(P<0.05),图8~10同。

Fig.7 Effect of algae powder on SOD activity of wheat under salt stress

图8 藻粉对盐胁迫下小麦过氧化物酶活性的影响

Fig.8 Effect of algae powder on POD activity of wheat under salt stress

图9 藻粉对盐胁迫下小麦过氧化氢酶活性的影响

Fig.9 Effect of algae powder on CAT activity of wheat under salt stress

图10 藻粉对盐胁迫下小麦丙二醛含量的影响

Fig.10 Effect of algae powder on MDA content of wheat under salt stress

图11 藻粉对盐胁迫下小麦幼苗Na+/K+的影响

Fig.11 Effect of algae powder on Na+/K+ in wheat seedlings under salt stress

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